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The effect of acidosis on the labelling of urinary ammonia during infusion of [amide-15N]glutamine in human subjects

Published online by Cambridge University Press:  09 March 2007

J. C. Waterlow ,
A. A. Jackson ,
M. H. N. Golden ,
F. Jahoor ,
G. Sutton  and
E. B. Fern
J. C. Waterlow
Affiliation:
Department of Public Health and Policy, London School of Hygiene and Tropical Medicine, Keppel Street, London WClE 7HT
A. A. Jackson
Affiliation:
Institute of Human Nutrition, University of Southampton, Bassett Crescent East, Southampton SO9 3TU
M. H. N. Golden
Affiliation:
Tropical Metabolism Research Unit, University of West Indies, Jamaica, West Indies
F. Jahoor
Affiliation:
Tropical Metabolism Research Unit, University of West Indies, Jamaica, West Indies
G. Sutton
Affiliation:
Institute of Human Nutrition, University of Southampton, Bassett Crescent East, Southampton SO9 3TU
E. B. Fern
Affiliation:
Nestec Ltd, Vevey, Switzerland
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Abstract

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In three experiments [amide-15N]glutamine was infused intravenously in male volunteers. After 4–8 h of infusion acidosis was achieved by an oral dose of CaCl2 (1 mmol/kg). In one subject acidosis was maintained for 5 d. The acid load produced an approximately 3-fold increase in urinary NH3 excretion, with a small (approximately 20%) and transient increase in the isotope abundance of urinary NH3 Estimates of glutamine production rate (flux) were obtained in two experiments. There was no evidence that it was increased in acidosis. The extra NH3 production by the kidney represented only a very small part, about 3%, of the total glutamine production rate.

Keywords

AcidosisGlutamine metabolismUrinary ammonia
Type
Labelling of urinary ammonia in glutamine infusion
Information
British Journal of Nutrition , Volume 72 , Issue 1 , July 1994 , pp. 83 - 92
Copyright
Copyright © The Nutrition Society 1994

References

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