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The effect of combined dietary iron, calcium and folk acid supplementation on apparent 65Zn absorption and zinc status in pregnant rats

Published online by Cambridge University Press:  09 March 2007

Susan Southon ,
A. J. A. Wright  and
Susan J. Fairweather−Tait
Susan Southon
Affiliation:
AFRC Institute of Food Research, Norwich Laboratory, Colney Lane, Norwich NR4 7UA
A. J. A. Wright
Affiliation:
AFRC Institute of Food Research, Norwich Laboratory, Colney Lane, Norwich NR4 7UA
Susan J. Fairweather−Tait
Affiliation:
AFRC Institute of Food Research, Norwich Laboratory, Colney Lane, Norwich NR4 7UA
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Abstract

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In the present study the effect of combined iron, calcium and folic acid supplementation of the diet on 65Zn retention and zinc status was studied in the pregnant rat. Female Wistar rats were fed on a low-(8 μg/g) or high- (60 μg/g) Zn diet for 14 d and then mated overnight. After mating, half the rats were fed on the low- or high-Zn diet as before, whilst the other half were fed on similar diets supplemented with Fe, Ca and folic acid. The level of supplementation was chosen to reflect proportionately the possible increase in daily intakes of these nutrients by pregnant women. Rats which did not mate successfully were used as non-pregnant controls. On day 18 of pregnancy, each animal was given a meal of the appropriate diet labelled extrinsically with 65Zn, and on day 20 rats were killed. Carcass 65zn retention was lower in pregnant and non-pregnant rats fed on the supplemented diets compared with those fed on the unsupplemented diets. Rats which consumed the supplemented diets throughout pregnancy had reduced plasma Zn concentrations but femur and fetal Zn concentrations were unaffected. Maternal femur Ca and fetal Fe concentrations were lower in the high-Zn groups compared with rats fed on low-Zn diets. It was concluded that the risk of inducing fetal Zn depletion as a consequence of Fe, Ca and folic acid supplementation during pregnancy appeared to be slight. However, significant differences in 65Zn retention and maternal plasma Zn concentration in the supplemented groups, and reduced maternal bone Ca deposition and fetal Fe accretion in the high-Zn groups, indicated that it would seem wise to adopt a cautious approach to routine supplementation with individual minerals during pregnancy.

Keywords

Dietary supplementationPregnancyZincRat
Type
Lipids
Information
British Journal of Nutrition , Volume 62 , Issue 2 , September 1989 , pp. 415 - 423
Copyright
Copyright © The Nutrition Society 1989

References

REFERENCES

American Institute of Nutrition ad hoc Committee on Standards for Nutritional Studies (1977). Report of the American Institute of Nutrition Committee on Standards for Nutritional Studies. Journal of Nutrition 107, 1340–1348.CrossRefGoogle Scholar
Department of Health and Social Security (1979). Recommended daily amounts of food energy and nutrients for groups of people in the United Kingdom, Report on Health and Social Subjects no. 15. London: H.M. Stationery Office.Google Scholar
Fairweather-Tait, S. J. & Southon, S. (1989). Studies of iron:zinc interactions in adult rats and the effect of iron fortification of two commercial infant weaning products on iron and zinc status of weanling rats. Journal of Nutrition 119, 599–606.CrossRefGoogle ScholarPubMed
Fairweather-Tait, S. J. & Wright, A. J. A. (1984). The influence of previous iron intake on the estimation of bioavailability of Fe from a test meal given to rats. British Journal of Nutrition 51, 185–191.CrossRefGoogle ScholarPubMed
Fairweather-Tait, S. J., Wright, A. J. A., Cooke, J. & Franklin, J. (1985). Studies of zinc metabolism in pregnant and lactating rats. British Journal of Nutrition 54, 401–413.CrossRefGoogle ScholarPubMed
Forbes, R. M. (1964). Mineral utilization in the rat. III. Effects of calcium, phosphorus, lactose and sources of protein in zinc-deficient and zinc-adequate diets. Journal of Nutrition 83, 225–233.CrossRefGoogle Scholar
Hambidge, K. M., Krebs, N. F., Jacobs, M. A., Favier, A., Guyette, L. & Ikle, D. N. (1983). Zinc nutritional status during pregnancy: a longitudinal study. American Journal of Clinical Nutrition 37, 429–442.CrossRefGoogle ScholarPubMed
Heth, D. A. & Hoekstra, W. G. (1965). Zinc-65 absorption and turnover in rats. I. A procedure to determine zinc-65 absorption and the antagonistic effect of calcium in a practical diet. Journal of Nutrition 85, 367–374.CrossRefGoogle Scholar
Huber, A. M. & Gershoff, S. N. (1970). Effects of dietary zinc and calcium on the retention and distribution of zinc in rats fed semi-purified diets. Journal of Nutrition 100, 949–954.CrossRefGoogle Scholar
Hurley, L. S. (1969). Zinc deficiency in the developing rat. American Journal of Clinical Nutrition 22, 1332–1339.CrossRefGoogle ScholarPubMed
Kirchgessner, M., Schwarz, F. J. & Roth-Maier, D. A. (1982). Changes in the metabolism of copper, zinc and manganese in gravidity and lactation. In Trace Element Metabolism in Man and Animals, pp. 85–88 [Gawthorne, J. M.Howell, J. M. C. and White, C. L. editors]. Berlin, Heidelberg, New York: Springer-Verlag.Google Scholar
Krebs, N. F., Hambidge, K. M., Hagerman, R. J., Peirce, P. L., Johnson, K. M., English, J. L., Miller, L. L. & Fennessey, P. V. (1989). Effect of pharmacological doses of folate on zinc absorption and status. In Nutrient Availability [Southgate, D. A. T.Johnson, I. T. and Fenwick, G. R. editors]. Cambridge: Royal Society of Chemistry (In the Press).Google Scholar
McKenzie-Parnell, J. M., Wilson, P. D. & Spears, G. F. S. (1988). Effect of iron supplementation on zinc status and the outcome of pregnancy. In Trace Element Metabolism in Man and Animals–6, pp. 593–594. [Hurley, M. S.Keen, C. L.Lönnerdal, B. and Rucker, R. B. editors]. New York: Plenum Publishing Co.CrossRefGoogle Scholar
Magee, A. C. & Chang Fu, S. (1979). Effects of calcium and phosphorus supplements on mineral balances of young zinc-fed rats. Nutrition Reports International 19, 343–351.Google Scholar
Masters, D. G., Keen, C. L., Lönnerdal, B. & Hurley, L. S. (1983). Zinc deficiency teratogenicity: the protective role of maternal tissue catabolism. Journal of Nutrition 113, 905–912.CrossRefGoogle ScholarPubMed
Masters, D. G., Keen, C. L., Lönnerdal, B. & Hurley, L. S. (1986). Release of zinc from maternal tissues during zinc deficiency or simultaneous zinc and calcium deficiency in the pregnant rat. Journal of Nutrition 116, 2148–2154.CrossRefGoogle ScholarPubMed
Milne, D. B., Canfield, W. K., Mahalko, J. R. & Sandstead, H. H. (1984). Effect of oral folic acid supplements on zinc, copper and iron absorption and excretion. American Journal of Clinical Nutrition 39, 535–539.CrossRefGoogle ScholarPubMed
National Research Council (1980). Recommended Dietary Allowances, 9th revised ed. Washington, DC: National Academy of Sciences.Google Scholar
O'Dell, B. L. (1969). Effect of dietary components upon zinc availability. American Journal of Clinical Nutrition 10, 1315–1322.CrossRefGoogle Scholar
Sandstrom, B. K., Kivisto, B. & Cederblad, A. (1987). Absorption of zinc from soya protein meals in humans. Journal of Nutrition 117, 321–327.CrossRefGoogle Scholar
Simmer, K., James, C. & Thompson, R. P. H. (1987). Are iron–folate supplements harmful? American Journal of Clinical Nutrition 45, 122–125.CrossRefGoogle ScholarPubMed
Simmer, K. & Thompson, R. P. H. (1985). Zinc in the fetus and newborn. Acta Paediatrica Scandinavica 319, Suppl., 158–163.CrossRefGoogle ScholarPubMed
Solomons, N. W. (1986). Competitive interactions of iron and zinc in the diet: consequences for human nutrition. Journal of Nutrition 116, 927–935.CrossRefGoogle ScholarPubMed
Van Kampen, E. J. & Zilstra, W. G. (1961). Standardisation of hemoglobinometry. II. The hemoglobincyanide method. Clinica Chimica Acta 6, 538–544.CrossRefGoogle Scholar