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Effect of feed intake on amino acid transfers across the ovine hindquarters

Published online by Cambridge University Press:  09 March 2007

S. O. Hoskin ,
I. C. Savary-Auzeloux ,
A. G. Calder ,
G. Zuur  and
G. E. Lobley
S. O. Hoskin*
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, Scotland, UK
I. C. Savary-Auzeloux
Affiliation:
INRA – Centre de Clermont-Ferrand/Theix, 63122 Saint Genes-Champanelle, France
A. G. Calder
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, Scotland, UK
G. Zuur
Affiliation:
Biomathematics and Statistics Scotland, Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, Scotland, UK
G. E. Lobley
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, Scotland, UK
*
*Corresponding author: Dr Simone O. Hoskin, present address, Institute of Food, Nutrition and Human Health, Massey University, Private Bag 11222, Palmerston North, New Zealand, fax +64 6 3505684, email S.O.Hoskin@massey.ac.nz
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Abstract

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Responses in variables of amino acid (AA) metabolism across peripheral tissues to feed intake were studied in six sheep (mean live weight 32 kg) prepared with arterio–venous catheters across the hindquarters. Four intakes (0·5, 1·0, 1·5 and 2·5 × maintenance energy) were offered over 2-week periods to each sheep in a Latin square design with two animals replicated. Animals were infused intravenously with a mixture of U-13C-labelled AA for 10 h and integrated blood samples withdrawn from the aorta and vena cava hourly between 5 and 9 h of infusion. Biopsy samples were also taken from skin and m. vastus lateralis. Data from both essential (histidine, isoleucine, leucine, lysine, phenylalanine, threonine) and nonessential (glycine, proline, serine, tyrosine) AA were modelled to give rates of inward and outward transport, protein synthesis and degradation, plus the fraction of total vascular inflow that exchanged with the hindquarter tissues. Rates of inward transport varied more than 10-fold between AA. For all essential AA (plus serine), inward transport increased with food intake (P<0·04). There were corresponding increases in AA efflux (P<0·05) from the tissues for threonine and the branched-chain AA. Protein synthesis rates estimated from the kinetics of these AA also increased with intake (P<0·02). Rates of inward transport greatly exceeded the amount of AA necessary to support protein retention, but were more similar to rates of protein synthesis. Nutritional or other strategies to enhance AA transport into peripheral tissues are unlikely to increase anabolic responses.

Keywords

Amino acid transportProtein metabolismFeed intakeHindlimbMuscleSkinSheep
Type
Research Article
Information
British Journal of Nutrition , Volume 89 , Issue 2 , February 2003 , pp. 167 - 179
Copyright
Copyright © The Nutrition Society 2003

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