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Effect of food intake on energy and protein metabolism in the skin of Romney sheep

Published online by Cambridge University Press:  09 March 2007

P. M. Harris ,
J. Lee ,
B. R. Sinclair ,
B. P. Treloar  and
M. P. Gurnsey
P. M. Harris
Affiliation:
AgResearch, Grasslands Research Centre, Private Bag 11008, Palmerston North, New Zealand
J. Lee
Affiliation:
AgResearch, Grasslands Research Centre, Private Bag 11008, Palmerston North, New Zealand
B. R. Sinclair
Affiliation:
AgResearch, Grasslands Research Centre, Private Bag 11008, Palmerston North, New Zealand
B. P. Treloar
Affiliation:
AgResearch, Grasslands Research Centre, Private Bag 11008, Palmerston North, New Zealand
M. P. Gurnsey
Affiliation:
AgResearch, Grasslands Research Centre, Private Bag 11008, Palmerston North, New Zealand
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Abstract

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Sheep fed on either a low (500 g lucerne (Medicago sativa) chaff/d; L) or high (1100 g lucerne chaff/d; H) intake had measurements made, using arterio–venous techniques, of blood flow and energy metabolite and cysteine utilization in the skin. Sheep on the H intake had significantly increased skin blood flow (P = 0.014) and oxygen uptake (P = 0.05). Although the H sheep had higher skin blood flow they showed no difference in skin uptake of either glucose or acetate compared with the L sheep, but the H sheep had a significantly lower output of lactate (P = 0.014). Animals in each group had either [14Clglucose or [14Clacetate infused into the skin which showed that acetate was the predominant precursor of skin sterol and fatty acid synthesis in the H sheep while L sheep skin used both glucose and acetate. The H sheep showed an increase in the net uptake of cysteine by the skin (P = 0.053), and in the uptake of cysteine for protein synthesis (P = 0.078), relative to the L sheep and this increase was of a comparable magnitude to the increase in blood now to the skin. Although blood flow, protein synthesis and energy supply increased in the skin of the H sheep by 200–300%, wool production would only have increased by 10–20 %, suggesting that nutrient flux changes are not the sole level of regulation of wool production.

Keywords

Skin energy metabolismCysteineFeed intakeSheep
Type
food intake on energy and protein metabolism in the skin of Romney sheep
Information
British Journal of Nutrition , Volume 71 , Issue 5 , May 1994 , pp. 647 - 660
Copyright
Copyright © The Nutrition Society 1994

References

Attaix, D., Aurosseau, E., Manghebati, A. & Arnal, M. (1988). Contribution of liver, skin, and skeletal muscle to whole-body protein synThesis in the young lamb. British Journal of Nutrition 60, 77–84.Google Scholar
Bird, A. R., Chandler, K. D. & Bell, A. W. (1981). Effects of exercise and plane of nutrition on nutrient utilisation by the hind limb of the sheep. Australian Journal of Biological Science 34, 541–550.Google Scholar
Chapman, R. E. & Ward, K. A. (1979). Histological and biochemical features of the wool fibre and follicle. In Physiological and Environmenral Limitations to Wool Growth, pp. 193–208 [Black, J. L. and Reis, P. J., editors]. Armidale, Australia: University of New England Publishing Unit.Google Scholar
Davis, S. R. & Collier, R. J. (1985). Mamindry blood flow and regulation of substrate supply for milk synThesis. Journal of Dairy Science 68, 1041–1058.Google Scholar
Dellow, D. W., Harris, P. M. & Sinclair, B. R. (1988). Measurement of blood flow in the skin of sheep. Proceedings of the New Zealand Nutrition Society 13, 130–133.Google Scholar
Grubb, B. R. & Mills, L. P. (1981). Blood oxygen content in microlitre samples using an easy to build galvanic oxygen cell. Journal of Applied Physiology 50, 456–464.Google Scholar
Harris, P. M., Dellow, D. W. & Sinclair, B. R. (1988). Measurement of blood flow and amino acid metabolism in the skin of sheep. Proceedings of the New Zealand Society of Animal Production 48, 187–189.Google Scholar
Harris, P. M., Dellow, D. W. & Sinclair, B. R. (1989). Preliminary in vivo measurements of protein and energy metabolism in the skin of sheep. Australian Journal of Agricultural Research 40, 879–888.Google Scholar
Harris, P. M., Lee, J., Gurnsey, M. P., Dellow, D. W. & Sinclair, B. R. (1993). Measurement of blood flow and metabolite uptake by the skin of fleeceweight-selected and control Romney rams. Australian Journal of Agricultural Research 41, 255–264.Google Scholar
Harris, P. M., Lee, J., Sinclair, B. R. & Treloar, B. P. (1994). The effect of whole body cysteine supplementation on cysteine utilisation by the skin of a well fed sheep. Proceedings of the New Zealand Society of Animal Production 54, (In the Press).Google Scholar
Harris, P. M., Lobley, G. E., Skene, P. A., Buchan, V., Milne, E., Calder, A. G., Garlick, P. J. & Connell, A. (1992). Effect of food intake on hind-limb and whole-body protein metabolism in young growing sheep: chronic studies based on arterio-venous techniques. British Journal of Nutrition 68, 389–407.Google Scholar
Lee, J., Harris, P. M., Sinclair, B. R. & Treloar, B. P. (1993). Whole body metabohm of cysteine and glutathione and their utilization in the skin of Romney sheep: consequences for wool growth. The Journal of Agricultural Science, Cambridge 121, 111–124.Google Scholar
Leng, R. A. & Stephenson, S. K. (1965). Glucose and acetate metabolism by isolated sheep wool follicles. Archives of Biochemistry and Biophysics 110, 8–15.Google Scholar
Lobley, G. E. (1990). Energy metabolism reactions in ruminant muscle: responses to age, nutrition, and hormonal status. Nutrition and Development 30, 13–34.Google Scholar
Lobley, G. E., Harris, P. M., Skene, P. A., Buchan, V., Milne, E., Calder, A. G., Anderson, S. E., Garlick, P. J. & Connell, A. (1992). Responses in tissue protein synThesis to sub- and supra-maintenance intake in young growing sheep: comparison of large-dose and continuous-infusion techniques. British Journal ofNutrition 68, 373–388.Google Scholar
MacRae, J. C. & Lobley, G. E. (1986). Interactions between energy and protein. In Control of Digestion and Metabolism in Ruminants, pp. 367–385 [Milligan, L. P., Grovum, W. L. and Dobson, A., editors]. Englewood Cliffs, NJ: Prentice Hall.Google Scholar
Oddy, V. H., Gooden, J. M. & Annison, E. F. (1984). Partitioning of nutrients in Merino ewes. I. Contribution of skeletal muscle, the pregnant uterus and the lactating mammary gland to total energy expenditure. Australian Journal of Biological Science 37, 351–88.Google Scholar
Oddy, V. H., Gooden, J. M., Hough, G. M., Teleni, E. & Annison, E. F. (1985). Partitioning of nutrients in Merino ewes. 11. Glucose utilization by skeletal muscle, the pregnant uterus and the lactating mammary gland in relation to whole body glucose utilization. Australian Journal of Biological Science 38, 95–108.Google Scholar
Oddy, V. H. & Lindsay, D. B. (1986). Determination of rates of protein synThesis, gain and degradation in intact hind limb muscle of lambs. Biochemical Journal 233, 417–425.Google Scholar
Statistical Analysis Systems (1985). SAS User's Guide: Statistics, Version 5. Cary, North Carolina: SAS Institute Inc.Google Scholar
Thornberry, K. J. & Atkins, K. D. (1984). Variation in the non wool components of the greasy fleece over the body of the Merino sheep. Australian Journal of Experimental Agriculture and Animal Husbandry 24, 72–76.Google Scholar