Hostname: page-component-78c5997874-ndw9j Total loading time: 0 Render date: 2024-11-13T07:42:00.372Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of meal fat quality on oxidation resistance of postprandial VLDL and LDL particles and plasma triacylglycerol level

Published online by Cambridge University Press:  09 March 2007

Nina S. Nielsen ,
Peter Marckmann  and
Carl-Erik Høy
Nina S. Nielsen*
Affiliation:
Department of Biochemistry and Nutrition, The Technical University of Denmark, Lyngby, Denmark Centre for Advanced Food Studies, The Technical University of Denmark, Lyngby, Denmark
Peter Marckmann
Affiliation:
Centre for Advanced Food Studies, The Technical University of Denmark, Lyngby, Denmark Research Department of Human Nutrition, The Royal Veterinary and Agricultural University, Copenhagen, Denmark
Carl-Erik Høy
Affiliation:
Department of Biochemistry and Nutrition, The Technical University of Denmark, Lyngby, Denmark Centre for Advanced Food Studies, The Technical University of Denmark, Lyngby, Denmark
*
*Corresponding author: Dr Nina S. Nielson, fax +45 45 88 63 07 email ninas@mimer.be.dtu.dk
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

This study was performed to examine the postprandial effects of meals containing dietary fats, with their natural fatty acid composition and tocopherol content, on the plasma triacylglycerols (TG) and tocopherols and on the resistance of VLDL and LDL to oxidation. On six separate days eighteen healthy male subjects were given low-fat meals (LF) or the LF meals enriched with sunflower oil (SO), rapeseed oil (RO), olive oil (OO), palm oil (PO), or butter (B) in a crossover design. The fat-rich meals all resulted in similar postprandial TG responses while the LF test meal did not increase plasma TG level. The postprandial plasma fatty acid profile changed to resemble the fatty acid composition of the ingested test fat. The α-tocopherol:γ-tocopherol ratios in postprandial plasma and VLDL samples were greater than in the test fats. We found that the resistance of VLDL particles to oxidation in the postprandial state as assessed from lag time was increased after the PO-rich meal as compared with the SO-rich meal (P=0·018), and the propagation rate was greater after the SO- and RO-rich meals compared with the others (P<0·001). The resistance of LDL particles to oxidation was unaffected by the meals. In postprandial VLDL samples, the content of α-tocopherol was greater after the OO- and SO-rich meals compared with the meal rich in PO (P=0·034 and 0·042 respectively). The γ-tocopherol content of VLDL was highest after RO-meal as compared with all other test meals (P=0·0019), and higher after SO as compared with B (P=0·0148). Large individual differences were noted. In conclusion, meals enriched with different fats lead to the formation of VLDL particles with varying resistance to oxidation.

Keywords

Dietary fatLipoproteinsOxidation resistancePolyunsaturated fatty acids
Type
Research Article
Information
British Journal of Nutrition , Volume 84 , Issue 6 , December 2000 , pp. 855 - 863
Copyright
Copyright © The Nutrition Society 2000

References

Asp, KG, Johansson, CG, Hallmer, H & Siljeström, M (1983) Rapid enzymatic assay of insoluble and soluble dietary fiber. Journal of Agricultural Food Chemistry 31, 876–882.CrossRefGoogle ScholarPubMed
Beisiegel, U & St Clair, RW (1996) An emerging understanding of the interactions of plasma lipoproteins with the arterial wall that leads to the development of atherosclerosis. Current Opinion in Lipidology 7, 265–268.CrossRefGoogle Scholar
Belcher, JD, Balla, J, Balla, G, Jacobs, DR, Gross, M, Jacob, HS & Vercellotti, GM (1993) Vitamin E, LDL, and endothelium. Brief oral vitamin supplementation prevents oxidized LDL-mediated vascular injury in vitro. Arteriosclerosis and Thrombosis 13, 1779–1789.CrossRefGoogle ScholarPubMed
Bergeron, N & Havel, RC (1995) Influence of diets rich in saturated and omega-6 polyunsaturated fatty acids on the postprandial responses of apolipoproteins B-48, B-100, E, and lipids in triglyceride-rich lipoproteins. Arteriosclerosis, Thrombosis and Vascular Biology 15, 2111–2121.CrossRefGoogle Scholar
Brussaard, HE, Leuven, JAG, Kluft, C, Krans, MJ, Duyvenvoorde, W, Buytenhek, R, Van der Laarse, A & Princen, HMG (1997) Effect of 17β-estradiol on plasma lipids and LDL oxidation in postmenopausal women with type II diabetes mellitus. Arteriosclerosis, Thrombosis and Vascular Biology 17, 324–330.CrossRefGoogle ScholarPubMed
Christopherson, SW & Glass, RL (1969) Preparation of milk fat methyl esters by alcoholysis in an essential nonalcoholic solution. Journal of Dairy Science 52, 1289–1290.CrossRefGoogle Scholar
Cohen, JC, Noakes, TD & Benade, AJS (1988) Serum triglyceride responses to fatty meals: effects of meal fat content. American Journal of Clinical Nutrition 47, 825–827.CrossRefGoogle ScholarPubMed
Corongiu, FP, Banni, S & Lombardi, B (1994) Quantitation of conjugated dienes by second-derivative UV-spectroscopy. Methods in Toxicology 1B, 415–420.Google Scholar
Dieber-Rotheneder, M, Puhl, H, Waeg, G, Striegl, G & Esterbauer, H (1991) Effect of oral supplementation with D-α-tocopherol on the vitamin E content of human low density lipoproteins and resistance to oxidation. Journal of Lipid Research 32, 1325–1332.CrossRefGoogle ScholarPubMed
Ebenbichler, CF, Kirchmair, R, Egger, C & Patch, JR (1995) Postprandial state and atherosclerosis. Current Opinion in Lipidology 6, 286–290.CrossRefGoogle ScholarPubMed
Esterbauer, H, Striegl, G, Puhl, H & Rotheneder, M (1989) Continuous monitoring of in vitro oxidation of human low density lipoprotein. Free Radical Research Communication 6, 67–75.CrossRefGoogle ScholarPubMed
Folch, J, Lees, M & Stanley, GHS (1957) A simple method for the isolation and purification of total lipides from animal tissues. Journal of Biological Chemistry 226, 497–509.CrossRefGoogle ScholarPubMed
Ginsberg, HN, Jones, J, Blaner, WS, Thomas, A, Karmally, W, Fields, L, Blood, D & Begg, MD (1995) Association of postprandial triglyceride and retinyl palmitate responses with newly diagnosed exercise-induced myocardial ischemia in middle-aged men and women. Arteriosclerosis, Thrombosis and Vascular Biology 15, 1829–1838.CrossRefGoogle ScholarPubMed
Granot, E, Tamir, I & Deckelbaum, RJ (1988) Neutral lipid transfer protein does not regulate α-tocopherol transfer between human plasma lipoproteins. Lipids 23, 17–21.CrossRefGoogle Scholar
Hau, M-F, Smelt, AHM, Bindels, AJGH, Sijbrands, EJG, Van der Laarse, A, Onkenhout, W, van Duyvenvoorde, W & Princen, HMG (1996) Effects of fish oil on oxidation resistance of VLDL in hypertriglyceridemic patients. Arteriosclerosis, Thrombosis and Vascular Biology 16, 1197–1202.CrossRefGoogle ScholarPubMed
Havel, RC (1997) Postprandial lipid metabolism: an overview. Proceedings of the Nutrition Society 56, 659–666.CrossRefGoogle ScholarPubMed
Kaplan, LA, Miller, JA, Stein, EA & Stampfer, MJ (1990) Simultaneous, high-performance liquid chromatographic analysis of retinol tocopherols lycopene, and α- and β-carotene in serum and plasma. Methods in Enzymology 189, 155–167.CrossRefGoogle ScholarPubMed
Kirsten, WJ & Hesselius, GU (1983) Rapid, automatic, high capacity Dumas determination of nitrogen. Microchemical Journal 28, 529–547.CrossRefGoogle Scholar
Kleinveld, HA, Naber, AHJ, Stalenhoef, AFH & Demacker, PNM (1993) Oxidation resistance, oxidation rate, and extent of oxidation of human low density lipoprotein depend on the ratio of oleic acid content to linoleic acid content: studies in vitamin E deficient subjects. Free Radical Biology & Medicine 15, 273–280.CrossRefGoogle ScholarPubMed
Larsen, LF, Bladbjerg, E-M, Jespersen, J & Marckmann, P (1997) Effects of dietary fat quality and quantity on postprandial activation of blood coagulation factor VII. Arteriosclerosis, Thrombosis and Vascular Biology 17, 2904–2909.CrossRefGoogle ScholarPubMed
Lechleitner, M, Hoppichler, F, Föger, B & Patsch, JR (1994) Low-density lipoproteins of the postprandial state induce cellular cholesteryl ester accumulation in macrophages. Arteriosclerosis and Thrombosis 14, 1799–1807.CrossRefGoogle ScholarPubMed
Losowsky, MS, Kelleher, J, Walker, BE, Davies, T & Smith, CL (1972) Intake and absorption of tocopherol. Annals of the New York Academy of Sciences 203, 212–222.CrossRefGoogle ScholarPubMed
Lowry, OH, Rosebrough, NJ, Farr, AL & Randall, RJ (1951) Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–275.CrossRefGoogle ScholarPubMed
Mata, P, Varela, O, Alonso, R, Lahoz, C, de Oya, M & Badimon, L (1997) Monounsaturated and polyunsaturated n-6 fatty acid-enriched diets modify LDL oxidation and decrease human coronary smooth muscle cell DNS synthesis. Arteriosclerosis, Thrombosis and Vascular Biology 17, 2088–2095.CrossRefGoogle Scholar
Meydani, M, Cohn, JS, Macauley, JB, McNamara, JR, Blumberg, JB & Schaefer, EJ (1989) Postprandial changes in the plasma concentration of α- and γ-tocopherol in human subjects fed a fat-rich meal supplemented with fat-soluble vitamins. Journal of Nutrition 119, 1252–1258.CrossRefGoogle ScholarPubMed
O'Byrne, DJ, O'Keefe, SF & Shireman, RB (1998) Low-fat, monounsaturated-rich diets reduce susceptibility of low density lipoproteins to peroxidation ex vivo. Lipids 33, 149–157.CrossRefGoogle Scholar
Patsch, JR, Miesenböck, G, Hopferwieser, T, Mühlberger, V, Knapp, E, Dunn, JK, Gotto, AM & Patch, W (1992) Relation of triglyceride metabolism and coronary artery disease. Studies in the postprandial state. Arteriosclerosis and Thrombosis 12, 1336–1345.CrossRefGoogle ScholarPubMed
Princen, HMG, Poppel, VG, Vogelezang, C, Buytenhek, R & Kok, FJ (1992) Supplementation with vitamin E but not β-carotene in vivo protects low density lipoprotein from lipid peroxidation in vitro. Effect of cigarette smoking. Arteriosclerosis and Thrombosis 12, 554–562.CrossRefGoogle Scholar
Princen, HMG, van Duyvenvoorde, W, Buytenhek, R, Van der Laarse, A, van Poppel, G, Leuven, JAG & van Hinsberg, VWM (1995) Supplementation with low doses of vitamin E protects LDL from lipid peroxidation in men and women. Arteriosclerosis, Thrombosis and Vascular Biology 15, 325–333.CrossRefGoogle ScholarPubMed
Rapp, JH, Lespine, A, Hamilton, RL, Colyvas, N, Chaumeton, AH, Tweedie-Hardman, J, Kotite, L, Kunitake, ST, Havel, RJ & Kane, JP (1994) Triglyceride-rich lipoproteins isolated by selected-affinity anti-apolipoprotein B immunosorption from human atherosclerotic plaque. Arteriosclerosis and Thrombosis 14, 1767–1774.CrossRefGoogle ScholarPubMed
Reaven, PD, Grasse, BJ & Tribble, DL (1994) Effects of linoleate-enriched and oleate-enriched diets in combination with α-tocopherol on the susceptibility of LDL and LDL subfractions to oxidative modification in humans. Arteriosclerosis and Thrombosis 14, 557–566.CrossRefGoogle ScholarPubMed
Reaven, PD & Witztum, JL (1993) Comparison of supplementation of RRR-α-tocopherol and racemic α-tocopherol in humans. Effects on lipid levels and lipoprotein susceptibility to oxidation. Arteriosclerosis and Thrombosis 13, 601–608.CrossRefGoogle ScholarPubMed
Schrezenmeir, J, Weber, P, Probst, R, Biesalski, HK, Luley, C, Prellwitz, W, Krause, U & Beyer, J (1992) Postprandial pattern of triglyceride rich lipoprotein in normal-weight humans after an oral lipid load: exaggerated triglycerides and altered insulin response in some subjects. Annals of Nutrition and Metabolism 36, 186–196.CrossRefGoogle ScholarPubMed
Sethi, S, Gibney, MJ & Williams, CM (1993) Postprandial lipoprotein metabolism. Nutrition Research Reviews 6, 161–183.CrossRefGoogle ScholarPubMed
Sørensen, NS, Marckmann, P, Høy, C-E, Duyvenvoorde, W & Princen, HMG (1998) Effect of fish-oil-enriched margarine on plasma lipids, low-density-lipoprotein particle composition, size, and susceptibility to oxidation. American Journal of Clinical Nutrition 68, 235–241.CrossRefGoogle ScholarPubMed
Traber, MG & Kayden, HJ (1989) Preferential incorporation of α-tocopherol vs γ-tocopherol in human lipoproteins. American Journal of Clinical Nutrition 49, 517–526.CrossRefGoogle ScholarPubMed
Tribble, DL, Thiel, PM, van den Berg, JJM & Krauss, RM (1995) Differing α-tocopherol oxidative lability and ascorbic acid sparing effects in buoyant and dense LDL. Arteriosclerosis, Thrombosis and Vascular Biology 15, 2025–2031.CrossRefGoogle ScholarPubMed
Turpeinen, AM, Alfthan, G, Valsta, L, Hietanen, E, Salonen, JT, Schunk, H, Nyyssönen, K & Mutanen, M (1995) Plasma and lipoprotein lipid peroxidation in humans on sunflower and rapeseed oil diets. Lipids 30, 485–492.CrossRefGoogle ScholarPubMed
Visioli, F, Bellomo, G, Montedoro, G & Galli, C (1995) Low density lipoprotein oxidation is inhibited in vitro by olive oil constituents. Atherosclerosis 117, 25–32.CrossRefGoogle ScholarPubMed
Winklerhofer-Roob, BM, Ziouzenkova, O, Puhl, H, Ellenmunter, H, Greiner, P, Müller, G, van't Hof, MA, Esterbauer, H & Shmerling, DH (1995) Impaired resistance to oxidation of low density lipoprotein in cystic fibrosis: improvement during vitamin E supplementation. Free Radical Biology & Medicine 19, 725–733.CrossRefGoogle Scholar
Ylä-Herttuala, S, Jaakkola, O, Ehnholm, C, Tikkanen, MJ, Solakivi, T, Särkioja, T & Nikkari, T (1988) Characterization of two lipoproteins containing apolipoproteins B and E from lesion-free human aortic intima. Journal of Lipid Research 29, 563–572.CrossRefGoogle Scholar
Ylä-Herttuala, S, Palinsky, W, Rosenfeld, ME, Parthasarathy, S, Carew, TE, Butler, SW, Witztum, JL & Steinberg, D (1989) Evidence for the presence of oxidatively modified low density lipoprotein in atherosclerotic lesions of rabbit and man. Journal of Clinical Investigation 84, 1086–1095.CrossRefGoogle ScholarPubMed
Zampelas, A, Peel, AS, Gould, BJ, Wright, J & Williams, CM (1994) Polyunsaturated fatty acids of the n-6 and n-3 series: effects on postprandial lipid and apolipoprotein levels in healthy men. European Journal of Clinical Nutrition 48, 842–848.Google ScholarPubMed