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The effect of moderately and severely restricted dietary magnesium intakes on bone composition and bone metabolism in the rat

Published online by Cambridge University Press:  09 March 2007

Annette Creedon ,
Albert Flynn  and
Kevin Cashman
Annette Creedon
Affiliation:
Department of Nutrition, University College, Cork, Republic of Ireland
Albert Flynn
Affiliation:
Department of Nutrition, University College, Cork, Republic of Ireland
Kevin Cashman*
Affiliation:
Department of Nutrition, University College, Cork, Republic of Ireland
*
*Corresponding author: Dr Kevin Cashman, fax +353 21 270244, email K.Cashman@UCC.ie
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Abstract

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Forty 3-week-old male rats, Wistar strain, average weight 59 g, were randomized by weight into five groups of eight rats each. Three groups were fed ad libitum on a semi-purified diet containing (per kg) 400 (adequate), 200 (moderately Mg-restricted) or 20 (severely Mg-restricted) mg Mg for 3 weeks while two groups were pair-fed with the Mg-adequate diet in the same quantities as those consumed by the two Mg-restricted groups respectively. While weight gains and food conversion efficiency values for the Mg-restricted groups were similar to those of the corresponding pair-fed control groups, serum and kidney Mg, and femoral dry weight were reduced by 70, 7 and 9 % respectively in the severely Mg-restricted group and were unaffected in the moderately Mg-restricted group. Significant reductions were observed in urinary pyridinoline (Pyr) (by 44 and 34 %) and deoxypyridinoline (Dpyr) levels (by 40 and 33 %) (markers of bone resorption), serum osteocalcin levels (by 46 and 28 %) (marker of bone formation), femoral Mg levels (by 52 and 14 %) and osteocalcin mRNA levels (by 46 and 22 %) compared with the corresponding pair-fed controls, in the severely and moderately Mg-restricted groups respectively, and these reductions, except for those in urinary Pyr and Dpyr, were more marked in the severely Mg-restricted group. Femoral Ca and P concentrations were unaffected by dietary Mg restriction. These results show that not only severe but also moderate dietary restriction of Mg over 21 d results in qualitative changes in bone (i.e. reduced Mg concentration) as well as in aberrant bone turnover in young growing rats (i.e. severely depressed rates of bone formation and bone resorption), which may impair bone development and bone strength.

Keywords

MagnesiumBiochemical markersBone
Type
Research Article
Information
British Journal of Nutrition , Volume 82 , Issue 1 , July 1999 , pp. 63 - 71
Copyright
Copyright © The Nutrition Society 1999

References

Alfrey, AC, Miller, NL & Trow, R (1974) Effect of age and magnesium depletion on bone magnesium pools in rats. Journal of Clinical Investigation 54, 1074–1081.CrossRefGoogle Scholar
American Institute of Nutrition (1977) Report of the American Institute ad hoc committee on standards for nutritional studies. Journal of Nutrition 107, 1340–1348.CrossRefGoogle Scholar
Anast, CS & Forte, LF (1983) Parathyroid function and magnesium depletion in the rat. Endocrinology 113, 184–189.CrossRefGoogle ScholarPubMed
Anast, CS & Gardner, DW (1981) Magnesium metabolism. In Disorders of Mineral Metabolism: Pathophysiology of Calcium, Phosphorus and Magnesium, pp. 423–522 [Bronner, J and Coburn, JW, editors]. New York, NY: Academic Press.Google Scholar
Black, D, Farqharson, C & Robins, SP (1989) Excretion of pyridinium crosslinks of collagen in ovariectomized rats as urinary markers for increased bone resorption. Calcified Tissue International 44, 343–347.CrossRefGoogle ScholarPubMed
Boskey, AL, Rimnac, CM, Bansal, M, Federman, M, Lian, J & Boyan, BD (1992) Effect of short-term hypomagnesemia on the chemical and mechanical properties of rat bone. Journal of Orthopaedic Research 10, 774–783.CrossRefGoogle ScholarPubMed
Bunce, GE & King, GA (1978) Isolation and partial characterization of kidney stone matrix induced by magnesium deficiency in the rat. Experimental and Molecular Pathology 28, 322–325.Google Scholar
Calabresi, E, Lasagni, L, Franceschelli, F, Bartolini, L & Serio, M (1994) Use of an internal standard to measure pyridinoline and deoxypyridinoline in urine (letter). Clinical Chemistry 40, 336–337.Google Scholar
Carpenter, TO, Mackowiak, SJ, Troiano, N & Gundberg, CM (1992) Osteocalcin and its message: relationship to bone histology in magnesium-deprived rats. American Journal of Physiology 263, E107–E114.Google Scholar
Clark, T & Belanger, LF (1967) The effects of alterations in dietary magnesium on calcium phosphorus and skeletal metabolism. Calcified Tissue Research 1, 204–218.Google Scholar
Cleveland, LE, Goldman, JD & Borrud, LG (1996) Data Tables: Results from USDA's 1994 Continuing Survey of Food Intakes by Individuals and 1994 Diet and Health Knowledge Survey. Beltsville, MD: Agriculture Research Service, U.S. Department of Agriculture.Google Scholar
Cohen, L (1988) Recent data on magnesium and osteoporosis. Magnesium Research 1, 85–87.Google Scholar
Cohen, L & Laor, A (1990) Correlation between bone magnesium concentration and magnesium retention in the intravenous magnesium load test. Magnesium Research 3, 271–274.Google ScholarPubMed
Colwell, R, Russell, RGG & Eastell, R (1993) Factors affecting the assay of urinary 3-hydroxypyridinium cross-links of collagen as markers of bone resorption. European Journal of Clinical Investigation 23, 341–349.CrossRefGoogle Scholar
Dallemagne, MJ & Fabry, C (1956) Structure of bone salts. In Bone Structure and Metabolism, pp. 14–32 [Walstenholme, GEW and O'Connor, CM, editors]. Boston, MA: Little, Brown, and Co.Google Scholar
Ducy, P, Desbois, C, Boyce, B, Pinero, G, Story, B, Dunstan, C, Smith, E, Bonadio, J, Goldstein, S, Gundberg, C, Bradley, A & Karsenty, G (1996) Increased bone formation in osteocalcin-deficient mice. Nature 382, 448–452.CrossRefGoogle ScholarPubMed
Egger, CD, Mühlbauer, RC, Felix, R, Delmas, PD, Marks, SC & Fleisch, H (1994) Evaluation of urinary pyridinium crosslink excretion as a marker of bone resorption in the rat. Journal of Bone and Mineral Research 9, 1211–1219.CrossRefGoogle ScholarPubMed
Elin, R (1969) Biochemical and pathological studies in magnesium deficiency in the ratPhD Thesis, University of Minnesota.Google Scholar
Fleet, JC & Hock, JM (1994) Identification of osteocalcin mRNA in nonosteoid tissue of rats and humans by reverse transcription-polymerase chain reaction. Journal of Bone and Mineral Research 9, 1565–1573.CrossRefGoogle ScholarPubMed
Gregory, J, Foster, K, Tyler, H & Wiseman, M (1990) The Dietary and Nutritional Survey of British Adults. London: H.M. Stationery Office.Google Scholar
Hahn, TJ, Chase, LR & Avioli, LV (1972) Effect of magnesium depletion on responsiveness to parathyroid hormone in parathyroidectomized rats. Journal of Clinical Investigation 51, 886–891.CrossRefGoogle ScholarPubMed
Heaton, FW (1965) Effect of magnesium deficiency on plasma alkaline phosphatase activity. Nature 207, 1292–1293.CrossRefGoogle ScholarPubMed
Héroux, D, Peter, D & Tanner, A (1975) Effect of chronic suboptimal intake of magnesium on magnesium and calcium content of bone and on bone strength of the rat. Canadian Journal of Physiology and Pharmacology 53, 304–310.Google Scholar
Institute of Medicine (1997) Dietary Intakes: Calcium, Magnesium, Phosphorus, Vitamin D, and Fluoride. Washington, DC: Food and Nutrition Board, National Academy Press.Google Scholar
Jones, JE, Schwartz, R & Krook, L (1980) Calcium homeostasis and bone pathology in magnesium deficient rats. Calcified Tissue International 31, 231–238.Google Scholar
Kenny, MA, McCoy, H & Williams, L (1994) Effects of magnesium deficiency on strength, mass, and composition of rat femur. Calcified Tissue International 54, 44–49.Google Scholar
Kimura, M, Ujihara, M & Yokoi, K (1996) Tissue manganese levels and liver pyruvate carboxylase activity in magnesium-deficient rats. Biological Trace Element Research 52, 171–179.Google Scholar
Kimura, M & Itokawa, Y (1989) Inefficient utilization of iron and minerals in magnesium deficient rats. In Magnesium in Health and Disease, pp. 95–102 [Itokawa, Y and Durlach, J, editors]. London: John Libbey.Google Scholar
Koh, ET, Reiser, S & Fields, M (1989) Dietary fructose as compared to glucose and starch increases the calcium content of kidney of magnesium-deficient rats. Journal of Nutrition 119, 1173–1178.Google Scholar
Lai, CC, Singer, L & Armstrong, WD (1975) Bone composition and phosphatase activity in magnesium deficiency in rats. Journal of Bone and Joint Surgery 57A, 516–522.Google Scholar
Larvor, P & Labat, M (1978) The influence of magnesium deficiency on calcium metabolism in the rat. Annales de Biologie Animale Biochimie Biophysique 18, 149–155.Google Scholar
Lerma, A, Planells, E, Aranda, P & Llopis, J (1993) Evolution of Mg deficiency in rats. Annals of Nutrition and Metabolism 37, 210–217.CrossRefGoogle ScholarPubMed
MacManus, J & Heaton, FW (1969) The effect of magnesium deficiency on calcium homeostasis in the rat. Clinical Science 36, 297–306.Google Scholar
MacManus, J, Heaton, FW & Lucas, PW (1971) A decreased response to parathyroid hormone in magnesium deficiency. Journal of Endocrinology 49, 253–258.CrossRefGoogle ScholarPubMed
Manicourt, DH, Orloff, S, Brauman, J & Schoutes, A (1981) Bone mineral content of the radius: good correlations with physiochemical determinations in iliac crest trabecular bone of normal and osteoporotic subjects. Metabolism 30, 57–62.CrossRefGoogle Scholar
Neuman, WF & Mulryan, FJ (1971) Synthetic hydroxyapatite crystals. IV. Magnesium incorporation. Calcified Tissue Research 7, 133–138.CrossRefGoogle ScholarPubMed
Pesce, A & Kaplan, LA (1987) Methods in Clinical Chemistry. St Louis, MO: C.V. Mosby Co.Google Scholar
Planells, E, Llopis, J, Perán, F & Aranda, P (1995) Changes in tissue calcium and phosphorus content and plasma concentrations of parathyroid hormone and calcitonin after long-term magnesium deficiency in rats. Journal of the American College of Nutrition 14, 292–298.CrossRefGoogle ScholarPubMed
Power, MJ & Fottrell, PF (1991) Osteocalcin: diagnostic methods and clinical applications. Critical Reviews in Clinical Laboratory Sciences 28, 287–335.Google Scholar
Rayssiguier, Y & Larvor, P (1978) Mineral bone composition and some elements of calcium metabolism in magnesium-deficient growing rats. Annales de Biologie Animale Biochimie Biophysique 18, 157–166.Google Scholar
Rayssiguier, Y, Thomasset, M, Garel, JM & Barlet, JP (1982) Plasma parathyroid hormone levels and intestinal calcium binding protein in magnesium-deficient rats. Hormone and Metabolic Research 14, 379–382.CrossRefGoogle ScholarPubMed
Reginster, JY, Strause, L, Deriosy, R, Lecart, MP, Saltman, P & Franchiomont, P (1989) Preliminary report of decreased serum magnesium in postmenopausal osteoporosis. Magnesium 8, 106–109.Google Scholar
Robins, SP & New, SA (1997) Markers of bone turnover in relation to bone health. Proceedings of the Nutrition Society 56, 903–914.Google Scholar
Robins, SP, Stead, DA & Duncan, A (1994) Precautions in using an internal standard to measure pyridinoline and deoxypyridinoline in urine [letter]. Clinical Chemistry 40, 2322–2323.CrossRefGoogle ScholarPubMed
Rude, RK (1998) Magnesium deficiency: a cause of heterogeneous disease in humans. Journal of Bone and Mineral Research 13, 749–758.Google Scholar
Snedecor, GW & Cochran, WG (1967) Statistical Methods. Ames, IA: Iowa State University Press.Google Scholar
Snyder, FH & Tweedy, WR (1942) The effects of magnesium-deficient diet on the serum phosphatase activity in the Albino rat. Journal of Biological Chemistry 146, 639–647.CrossRefGoogle Scholar
Sojka, JE & Weaver, CM (1995) Magnesium supplementation and osteoporosis. Nutrition Reviews 53, 71–74.Google Scholar
Stendig-Lindberg, G, Tepper, R & Leichter, I (1993) Trabecular bone density in a two year controlled trial of peroral magnesium in osteoporosis. Magnesium Research 6, 155–163.Google Scholar
Tucker, K, Kiel, DP, Hannan, MT & Felson, DT (1995) Magnesium intake is associated with bone mineral density (BMD) in elderly women. Journal of Bone Mineral Research 10, S466.Google Scholar
Trudeau, DL & Freier, EF (1967) Determination of Ca in urine and serum by atomic absorption spectrophotometry (AAS). Clinical Chemistry 13, C101–C114.CrossRefGoogle Scholar
Van Dokkum, W (1995) The intake of selected minerals and trace elements in European countries. Nutrition Research Reviews 8, 271–302.Google Scholar
Vormann, J, Förster, C, Zippel, U, Lozo, E, Günther, T, Merker, H-J & Stahlmann, R (1997) Effects of magnesium deficiency on magnesium and calcium content in bone and cartilage in developing rats in correlation to chondrotoxicity. Calcified Tissue International 61, 230–238.CrossRefGoogle ScholarPubMed
Wallach, S (1990) Effects of magnesium on skeletal metabolism. Magnesium 9, 1–14.Google Scholar
Walser, M (1967) Magnesium metabolism. Ergebnisse der Physiologie Biologischen Chemie und Experimentellen Pharmakologie 59, 186–296.Google Scholar
Weissman, N & Pileggi, VJ (1974) Inorganic ions. In Clinical Chemistry: Principles and Techniques, pp. 639–755 [Henry, RJ, Cannon, DC and Winkelman, JW, editors]. MD: Harper and Row.Google Scholar
Welsh, JJ & Weaver, VM (1988) Adaptation to low dietary calcium in magnesium-deficient rats. Journal of Nutrition 118, 729–734.Google Scholar