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Effect of multivitamins in an effervescent preparation on the respiratory burst of peritoneal macrophages in mice

Published online by Cambridge University Press:  09 March 2007

Judit Jakus ,
Tamás Kriska  and
Rozália Vanyúr
Judit Jakus*
Affiliation:
Institute of Chemistry, Chemical Research Center, Hungarian Academy of Sciences, 1525 Budapest, PO Box 17, Hungary
Tamás Kriska
Affiliation:
Institute of Chemistry, Chemical Research Center, Hungarian Academy of Sciences, 1525 Budapest, PO Box 17, Hungary
Rozália Vanyúr
Affiliation:
Institute of Chemistry, Chemical Research Center, Hungarian Academy of Sciences, 1525 Budapest, PO Box 17, Hungary
*
*Corresponding author: Dr Judit Jakus, fax +361 325 7554, email jakus@chemres.hu
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Abstract

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The effect of regular intake of low doses of an effervescent multivitamin preparation on the free-radical-producing activity of murine peritoneal macrophages under conditions resembling a possible infection was studied in vitro. Initially, several groups of mice were fed a basal diet and given, for 2 weeks, water without or with supplementation of either α -tocopherol, ascorbic acid, riboflavin or a multivitamin preparation. The supplementation period was followed by a 2-week wash-out time interval during which control and multivitamin groups received deionized water. Macrophage stimulation tests using chemiluminescent spectroscopy were performed at the end of the supplementation and wash-out periods to determine cell counts and their capacity to produce free radicals. Multivitamin supplementation increased the number, and the reactive oxygen species-producing activity, of macrophages. This effect persisted for 2 weeks after higher doses of supplementation were stopped. Multivitamin supplementation lowered the steady-state free radical concentrations of liver and spleen as measured by electron paramagnetic resonance spectroscopy. It also increased the antioxidant reactivity of the same organs, while there was no effect on the free radical concentration and antioxidant capacity of the kidney and brain. When taken regularly, low doses of multivitamin supplementation may have a beneficial effect on the defence mechanisms of the organism.

Keywords

VitaminsReactive oxygen speciesChemiluminescenceRespiratory burst
Type
Research Article
Information
British Journal of Nutrition , Volume 87 , Issue 5 , May 2002 , pp. 501 - 508
Copyright
Copyright © The Nutrition Society 2002

References

Abrams, B, Duncan, D & Hertz-Picciotto, I (1993) A prospective study of dietary intake and acquired immune deficiency syndrome in HIV-seropositive homosexual men. Journal of Acquired Immune Deficiency Syndromes 6, 949–958.Google ScholarPubMed
Arquette, D & Caren, LD (1992) Effect of sodium ascorbate on the chemiluminescent response of murine peritoneal exudate cells. Life Sciences 50, 753–759.CrossRefGoogle ScholarPubMed
Babior, MB (1978) Oxygen-dependent microbial killing in phagocytes. New England Journal of Medicine 298, 659–668.CrossRefGoogle ScholarPubMed
Badwey, JA & Karnovsky, ML (1980) Active oxygen species and the functions of phagocytic leukocytes. Annual Review of Biochemistry 49, 695–726.CrossRefGoogle ScholarPubMed
Bendich, A (1996) Antioxidant vitamins and human immune responses. Vitamins and Hormones 52, 35–62.CrossRefGoogle ScholarPubMed
Bjørneboe, A, Nenseter, MS, Hagen, BF, Bjørneboe, GE, Prydz, K & Drevon, CA (1991) Effect of dietary deficiency and supplementation with all-rac-α-tocopherol on hepatic content in rats. Journal of Nutrition 121, 1208–1213.CrossRefGoogle ScholarPubMed
Böhles, H (1997) Antioxidative vitamins in prematurely and maturely born infants. International Journal for Vitamin and Nutrition Research 67, 321–328.Google ScholarPubMed
Brown, RE (1977) Interaction of nutrition and infection in clinical practice. Pediatric Clinics of North America 24, 241–252.CrossRefGoogle ScholarPubMed
Burkholder, WJ & Swecker, WS Jr (1990) Nutritional influences on immunity. Seminars in Veterinary Medicine and Surgery (Small Animals) 5, 154–166.Google ScholarPubMed
Castranova, V, Van Scott, MR & Van Dyke, K (1987) Isolation and identification of phagocytic cells. In Cellular Chemiluminescence, vol. I, pp. 25–38 [Van Dyke, K and Castranova, V, editors]. Boca Raton, FL: CRC Press.Google Scholar
Chan, AC (1993) Partners in defense, vitamin E and vitamin C. Canadian Journal of Physiology and Pharmacology 71, 725–731.CrossRefGoogle ScholarPubMed
Chandra, RK (1999) Nutrition and immunology: from the clinic to cellular biology and back again. Proceedings of the Nutrition Society 58, 681–683.CrossRefGoogle Scholar
Chandra, RK & Kumari, S (1994) Nutrition and immunity: an overview. Journal of Nutrition 124, Suppl. 8, 1433S–1435S.CrossRefGoogle ScholarPubMed
Chen, H & Tappell, AL (1995) Protection of vitamin E, selenium, trolox C, ascorbic acid palmitate, acetylcysteine, coenzyme Q10, beta-carotene, canthaxanthin, and (+)-catechin against oxidative damage to rat blood and tissue in vivo. Free Radical Biology and Medicine 18, 949–953.CrossRefGoogle Scholar
Chew, BC (1995) Antioxidant vitamins affect food animal immunity and health. Journal of Nutrition 125, 1804S–1808S.Google ScholarPubMed
Del Rio, M, Ruedas, G, Medina, S, Victor, VM & De la Fuente, M (1998) Improvement by several antioxidants of macrophage function in vitro. Life Sciences 63, 871–881.CrossRefGoogle ScholarPubMed
de Zwart, LL, Meerman, JHN, Commandeur, JNM & Vermeulen, NPE (1999) Biomarkers of free radical damage. Applications in experimental animals and in humans. Free Radical Biology and Medicine 26, 202–226.CrossRefGoogle ScholarPubMed
Eberlein-König, B, Placzek, M & Przybilla, B (1998) Protective effect against sunburn of combined systemic ascorbic acid (vitamin C) and D-α-tocopherol (vitamin E). Journal of the American Academy of Dermatology 38, 45–48.CrossRefGoogle ScholarPubMed
Fehér, J, Vereckei, A & Lengyel, G (1992) Role of free radical reactions in liver diseases. Acta Physiologica Hungarica 80, 351–361.Google ScholarPubMed
Fox, ES, Brower, JS, Bellezzo, JM & Leingang, KA (1997) N-acetylcysteine and α-tocopherol reverse the inflammatory response in activated rat Kupffer cells. Journal of Immunology 158, 5418–5423.CrossRefGoogle ScholarPubMed
Gál, D, Kriska, T & Maltseva, E (1997) In vivo experimental studies on the role of free radicals in photodynamic therapy. III. Photodynamic effect on free radicals generated in cell cultures. Biochemical and Biophysical Research Communications 233, 173–176.CrossRefGoogle ScholarPubMed
Gilligan, DM, Sack, MN, Gietta, V, Casino, PR, Quyyumi, AA, Rader, DJ, Panza, JA & Cannon, RO (1994) Effect of antioxidant vitamins on low density lipoprotein oxidation and impaired endothelium-dependent vasodilation in patients with hypercholesterolemia. Journal of the American College of Cardiology 24, 1611–1617.CrossRefGoogle ScholarPubMed
Gore, AB & Qureshi, MA (1997) Enhancement of humoral and cellular immunity by vitamin E after embryonic exposure. Poultry Science 76, 984–991.CrossRefGoogle ScholarPubMed
Hannigan, BM (1994) Diet and immune function. British Journal of Biomedical Sciences 51, 252–259.Google ScholarPubMed
Hatchigian, EA, Santos, JL, Broitman, SA & Vitale, JJ (1989) Vitamin A supplementation improves macrophage function and bacterial clearance during experimental salmonella infection. Proceedings of the Society for Experimental Biology and Medicine 191, 47–54.CrossRefGoogle ScholarPubMed
Hauck, CR, Lorenzen, D, Saas, J & Meyer, TF (1997) An in vitro-differentiated human cell line as a model system to study the interaction of Neisseria gonorrhoeae with phagocytic cells. Infection and Immunity 65, 1863–1869.CrossRefGoogle Scholar
Hughes, DA (1999) Effects of dietary antioxidants on the immune function of middle-aged adults. Proceedings of the Nutrition Society 58, 79–84.CrossRefGoogle ScholarPubMed
Keusch, GT (1998) Nutrition and immunity: from A to Z. Nutrition Reviews 56, S3–S4.CrossRefGoogle ScholarPubMed
Knudsen, CA, Tappel, AL & North, JA (1996) Multiple antioxidants protect against heme protein and lipid oxidation in kidney tissue. Free Radical Biology and Medicine 20, 165–173.CrossRefGoogle ScholarPubMed
Kriska, T, Gamaley, I, Vasvári, G, Holly, S, Maltseva, E, Strizhak, P & Gál, D (1999) Quantitative studies on the respiratory burst generated in peritoneal macrophages. Journal of Photochemistry and Photobiology, Part B: Biology 50, 159–165.CrossRefGoogle ScholarPubMed
Lissi, E, Salim-Hanna, M, Pascual, C & del Castillo, MD (1995) Evaluation of total antioxidant potential (TRAP) and total antioxidant reactivity from luminol-enhanced chemiluminescence measurements. Free Radical Biology and Medicine 18, 153–158.CrossRefGoogle ScholarPubMed
McCall, MR & Frei, B (1999) Can antioxidant vitamins maternally reduce oxidative damage in humans? Free Radical Biology and Medicine 26, 1034–1053.CrossRefGoogle Scholar
Matthews, KS, Mrowczynski, E & Matthews, R (1994) Dietary deprivation of B-vitamins reflected in murine splenocyte proliferation in vitro. Biochemical and Biophysical Research Communications 198, 451–458.CrossRefGoogle ScholarPubMed
Meydani, M, Lipman, RD, Han, SN, Wu, D, Beharka, A, Martin, KR, Bronson, R, Cao, G, Smith, D & Meydani, SN (1998) The effect of long-term dietary supplementation with antioxidants. Annals of the New York Academy of Sciences 854, 352–360.CrossRefGoogle ScholarPubMed
Moriguchi, S, Kobayashi, N & Kishino, Y (1990) High dietary intakes of vitamin E and cellular immune functions in rats. Journal of Nutrition 120, 1096–1102.CrossRefGoogle ScholarPubMed
Moriguchi, S, Werner, L & Watson, RR (1985) High dietary vitamin A (retinyl palmitate) and cellular immune functions in mice. Immunology 56, 169–177.Google ScholarPubMed
Mosca, L, Rubenfire, M, Mandel, C, Rock, C, Tarshis, T, Tsai, A & Pearson, T (1997) Antioxidant nutrient supplementation reduces the susceptibility of low density lipoprotein to oxidation in patients with coronary artery disease. Journal of the American College of Cardiology 30, 392–399.CrossRefGoogle ScholarPubMed
Mulero, V, Esteban, MA & Meseguer, J (1998) Effects of in vitro addition of exogenous vitamins C and E in gilthead seabream (Sparus aurata L.) phagocytes. Veterinary Immunology and Immunopathology 66, 185–199.CrossRefGoogle Scholar
National Research Council (2001) Recommended Dietary Allowances, 11th edn. Washington, DC: National Academy Press.Google Scholar
Németh, A, Jakus, J, Kriska, T, Keszler, Á., Vanyúr, R & Gál, D (1999) Physico chemical modeling of the role of free radicals in photodynamic therapy. IV. Quantitative aspects of photodynamic effects on free radicals generated in cell cultures. Biochemical and Biophysical Research Communications 255, 360–366.CrossRefGoogle ScholarPubMed
Nikki, E (1987) Interaction of ascorbate and α-tocopherol. Annals of the New York Academy of Sciences 498, 186–199.CrossRefGoogle Scholar
Nyyssonen, K, Porkkala, E, Salonen, R, Korpela, H & Salonen, JT (1994) Increase in oxidation resistance of atherogenic serum lipoproteins following antioxidant supplementation: a randomized double-blind placebo-controlled clinical trial. European Journal of Clinical Nutrition 48, 633–642.Google ScholarPubMed
Palozza, P & Krinsky, NI (1992) β-Carotene and α-tocopherol are synergistic antioxidants. Archives of Biochemistry and Biophysics 297, 184–187.CrossRefGoogle ScholarPubMed
Reynolds, ES & Moslen, MT (1980) Free Radicals in Biology, vol. IV, pp. 49–94 [Pryor, WA, editor]. London, UK: Academic Press Inc.CrossRefGoogle Scholar
Rockenbauer, A & Korecz, L (1996) Automatic computer simulation of ESR spectra. Applied Magnetic Resonance 10, 29–43.CrossRefGoogle Scholar
Rücker, M, Finckh, B, Kohlschütter, A, Menger, MD & Vollmar, B (1997) Dietary vitamin E does not protect from endotoxin-induced hepatic microvascular dysfunction. Cellular and Molecular Life Sciences 53, 294–302.CrossRefGoogle Scholar
Sharmanov, TSh (1982) Nutrition and immunity. Voprosy Pitania 5, 3–8.Google Scholar
Shulyakovskaya, T, Sümegi, L & Gál, D (1993) In vivo experimental studies on the role of free radicals in photodynamic therapy. I. Measurement of the steady state concentration of free radicals in tumor tissues of mice. Biochemical and Biophysical Research Communications 195, 581–587.CrossRefGoogle ScholarPubMed
Surai, PF, Noble, RC & Speake, BK (1996) Tissue-specific differences in antioxidant distribution and susceptibility to lipid peroxidation during development of the chick embryo. Biochimica et Biophysica Acta 1304, 1–10.CrossRefGoogle ScholarPubMed
Tappell, AL (1962) Vitamin E as the biological lipid antioxidant. Vitamins and Hormones 20, 493–510.CrossRefGoogle Scholar
Thomas, SR, Neuzil, J & Stocker, R (1996) Cosupplementation with coenzyme Q prevents the prooxidant effect of α-tocopherol and increases the resistance of LDL to transition metal-dependent oxidation initiation. Arteriosclerosis, Thrombosis and Vascular Biology 16, 687–696.CrossRefGoogle ScholarPubMed