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The effect of various vitamin E derivatives on the urokinase-plasminogen activator system of ovine macrophages and neutrophils

Published online by Cambridge University Press:  09 March 2007

I. Politis ,
A. Voudouri ,
I. Bizelis  and
G. Zervas
I. Politis*
Affiliation:
Department of Animal Science, Agricultural University of Athens, Athens 118 55, Greece
A. Voudouri
Affiliation:
Department of Animal Science, Agricultural University of Athens, Athens 118 55, Greece
I. Bizelis
Affiliation:
Department of Animal Science, Agricultural University of Athens, Athens 118 55, Greece
G. Zervas
Affiliation:
Department of Animal Science, Agricultural University of Athens, Athens 118 55, Greece
*
*Corresponding author: Dr I. Politis, fax +30 1 529 4413, email i.politis@aua.gr
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Abstract

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The effect of vitamin E derivatives on the urokinase-plasminogen activator (u-PA) system of resting and phorbol myristate acetate (PMA)-activated ovine macrophages and neutrophils were investigated. Blood monocyte–macrophages and neutrophils were isolated from twenty-four animals. Macrophages or neutrophils were cultured in vitro for 3 or 24 h with or without various vitamin E derivatives: free α-tocopherol (α-T), α-tocopheryl acetate (α-TA), or α-tocopheryl succinate (α-TS). Following incubation, cells were stimulated with 80 μM-PMA. Total cell-associated u-PA, membrane-bound u-PA and free u-PA binding sites were determined before and after stimulation with PMA. Results showed that none of the vitamin E derivatives had any effect (P>0·05) on the u-PA system of resting monocyte–macrophages or neutrophils. In contrast, α-TS, but not α-TA or α-T, increased (P<0·01) total cell-associated u-PA and membrane-bound u-PA of PMA-stimulated macrophages and neutrophils. α-TS had no effect (P>0·05) on total u-PA and membrane-bound u-PA activities of macrophages and neutrophils cultured in the presence of 4-phorbol 12,13 didecanoate, a phorbol ester that does not activate protein kinase (PK) C. Addition of H7 (1-(5-isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride), which is a potent inhibitor of both PK A and C, completely abolished the effect of α-TS on total cell-associated u-PA and membrane-bound u-PA of PMA-activated macrophages and neutrophils. Addition of HA1004 (N-(2-quanidinoethyl)-5-isoquinoline sulfonamide hydrochloride), which is a potent PK A but a weak PK C inhibitor, had no effect (P>0·05) on total cell-associated u-PA and membrane-bound u-PA of PMA-activated macrophages and neutrophils cultured in the presence of α-TS. Thus, PK C modulates the effect of α-TS on the u-PA system of ovine macrophages and neutrophils.

Keywords

α-TocopherolUrokinase-plasminogen activatorProtein kinase C
Type
Research Article
Information
British Journal of Nutrition , Volume 89 , Issue 2 , February 2003 , pp. 259 - 265
Copyright
Copyright © The Nutrition Society 2003

References

Asano, T & Hidaka, H (1984) Vasodilatory action of HA 1004 [N-(2-quanidinoethyl)-5-isoquinoline sulfonamide hydrochloride], a novel calcium antagonist with no effect on cardiac function. Journal of Pharmacology and Experimental Therapeutics 231, 141–145.Google Scholar
Azzi, A, Breyer, I, Feher, M, Pastori, M, Ricciarelli, R, Spycher, S, Staffieri, M, Stocker, A, Zimmer, S & Zingg, JM (2000) Specific cellular responses to α-tocopherol. Journal of Nutrition 130, 1649–1652.CrossRefGoogle ScholarPubMed
Bendich, A (2001) Micronutrients in women's health and immune function. Nutrition 17, 858–867.CrossRefGoogle ScholarPubMed
Cachia, O, El Benna, J, Pedruzzi, E, Descomps, B, Gougerot-Podicalo, MA & Leger, CL (1998) α-Tocopherol inhibits the respiratory burst in human monocytes – attenuation of p47phox membrane translocation and phosphorylation. Journal of Biological Chemistry 273, 32801–32805.CrossRefGoogle ScholarPubMed
Cheeseman, KH, Holley, AE, Kelly, FJ, Wasil, M, Hughes, L & Burton, G (1995) Biokinetics in humans of RRR-alpha-tocopherol: the free phenol, acetate ester, and succinate ester forms of vitamin E. Free Radicals in Biology and Medicine 19, 591–598.CrossRefGoogle ScholarPubMed
De La Fuente, M & Victor, M (2000) Anti-oxidants as modulators of the immune system. Immunology and Cell Biology 78, 49–54.CrossRefGoogle Scholar
Devaraj, S, Li, D & Jialal, I (1996) The effects of alpha tocopherol supplementation on monocyte function. Decreased lipid oxidation, interleukin 1 beta secretion, and monocyte adhesion to endothelium. Journal of Clinical Investigation 98, 756–763.CrossRefGoogle ScholarPubMed
Erl, W, Weber, C, Wardemann, C & Weber, PC (1997) Alpha-tocopheryl succinate inhibits monocytic cell adhesion to endothelial cells by suppressing NF-kappa B mobilization. American Journal of Physiology 273, H634–H640.Google ScholarPubMed
Hidaka, H, Inagaki, M, Kawamoto, S & Sasaki, Y (1984) Isoquinoline-sulfonamides, novel and potent inhibitors of cyclic nucleotide dependent kinase and protein kinase C. Biochemistry 23, 5036–5041.CrossRefGoogle Scholar
Hidiroglou, N, McDowell, LR, Papas, AM, Antapi, M & Wilkinson, NS (1992) Bioavailability of vitamin E compounds in lambs. Journal of Animal Science 70, 2556–2561.CrossRefGoogle ScholarPubMed
Jensen, SK, Engberg, RM & Hendemann, MS (1999) All rac-α-tocopherol acetate is a better vitamin E source than all-rac-α-tocopherol succinate for broilers. Journal of Nutrition 129, 1355–1360.CrossRefGoogle ScholarPubMed
Kanno, T, Utsumi, T, Takehara, Y, Ide, A, Akiyama, J, Yoshioka, T, Horton, AA & Utsumi, K (1996) Inhibition of neutrophil-superoxide generation by α-tocopherol and coenzyme Q. Free Radical Research 24, 281–289.CrossRefGoogle ScholarPubMed
Kim, SJ, Bang, OS, Lee, YS & Kang, SS (1998) Production of inducible nitric oxide is required for monocytic differentiation of U937 cells induced by vitamin E-succinate. Journal of Cell Science 111, 435–441.CrossRefGoogle ScholarPubMed
Kline, K & Sanders, BG (1993) RRR-alpha-tocopheryl succinate inhibition of lectin-induced T cell proliferation. Nutrition and Cancer 19, 241–252.CrossRefGoogle ScholarPubMed
Lowe, WL, Yorek, MA, Karpen, CW, Teasdale, RM, Hovis, JG, Albrecht, B & Prokopiou, C (1992) Activation of protein kinase C differentially regulates insulin-like growth factor-I and basic fibroblast growth factor messenger RNA levels. Molecular Endocrinology 6, 741–752.Google ScholarPubMed
Plesner, T, Ploug, M, Ellis, V, Ronne, E, Hoyer-Hansen, G, Wittrup, M, Pedersen, TL, Tscherning, T, Dano, K & Hansen, NE (1994) The receptor for urokinase-type plasminogen activator and urokinase is translocated from two distinct intracellular compartments to the plasma membrane on stimulation of human neutrophils. Blood 83, 808–815.CrossRefGoogle Scholar
Politis, I (1996) Plasminogen activator system: Implications for mammary growth and involution. Journal of Dairy Science 79, 1097–1107.CrossRefGoogle ScholarPubMed
Politis, I (2000) The role of plasminogen activator in the bovine mammary gland. Advances in Experimental Biology 480, 203–207.CrossRefGoogle ScholarPubMed
Politis, I, Hidiroglou, N, Cheli, N & Baldi, N (2001) Effects of vitamin E on urokinase-plasminogen activator receptor expression by bovine neutrophils. American Journal of Veterinary Research 62, 1934–1938.CrossRefGoogle ScholarPubMed
Politis, I, Hidiroglou, N, White, JH, Gilmore, JA, Williams, SN, Scherf, H & Frigg, M (1996) Effects of vitamin E on mammary and blood leukocyte function, with emphasis on chemotaxis, in periparturient dairy cows. American Journal of Veterinary Research 57, 468–471.CrossRefGoogle ScholarPubMed
Rego, AC, Santos, MS, Proenca, MT & Oliveira, CR (1998) Influence of vitamin E on retinal cell survival. Toxicology 128, 113–124.CrossRefGoogle ScholarPubMed
Yoshikawa, T & Yoshida, N (2000) Vitamin E and leukocyte-endothelial interactions. Antioxidants in Redox Signaling 2, 821–825.CrossRefGoogle Scholar