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The effect of vitamin C or vitamin E supplementation on basal and H2O2-induced DNA damage in human lymphocytes

Published online by Cambridge University Press:  09 March 2007

Lisa A. Brennan ,
Gerard M. Morris ,
Gillian R. Wasson ,
Bernadette M. Hannigan  and
Yvonne A. Barnett
Lisa A. Brennan
Affiliation:
Cancer and Ageing Research Group, School of Biomedical Sciences, University of Ulster, Coleraine BT52 1SA, Northern Ireland
Gerard M. Morris
Affiliation:
Cancer and Ageing Research Group, School of Biomedical Sciences, University of Ulster, Coleraine BT52 1SA, Northern Ireland
Gillian R. Wasson
Affiliation:
Cancer and Ageing Research Group, School of Biomedical Sciences, University of Ulster, Coleraine BT52 1SA, Northern Ireland
Bernadette M. Hannigan
Affiliation:
Cancer and Ageing Research Group, School of Biomedical Sciences, University of Ulster, Coleraine BT52 1SA, Northern Ireland
Yvonne A. Barnett*
Affiliation:
Cancer and Ageing Research Group, School of Biomedical Sciences, University of Ulster, Coleraine BT52 1SA, Northern Ireland
*
*Corresponding author: Dr Yvonne Barnett, fax +44 (0) 1265 324965, email ya.barnett@ulst.ac.uk
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Abstract

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There is a wealth of epidemiological information on antioxidants and their possible prevention of disease progression but very little of the research on antioxidants has involved intervention studies. In this study, the potential protective effect of vitamin C or E supplementation in vivo against endogenous and H2O2-induced DNA damage levels in lymphocytes was assessed. The supplementation involved fourteen healthy male and female non-smokers mean age 25·53 (SD 1·82) years, who were asked to supplement an otherwise unchanged diet with 1000 mg vitamin C daily for 42 d or 800 mg vitamin E daily for 42 d. DNA damage in H2O2-treated peripheral blood lymphocytes (PBL) and untreated PBL before and after supplementation, and during a 6-week washout period was assessed using an ELISA. At each sampling time-point, the red cell concentrate activities of superoxide dismutase, catalase and glutathione peroxidase were also determined. Supplementation with vitamin C or vitamin E decreased significantly H2O2-induced DNA damage in PBL, but had no effect on endogenous levels of DNA damage. The activities of the antioxidant enzymes superoxide dismutase and glutathione peroxidase were suppressed during the supplementation period. These supplementation regimens may be used to limit the possible adverse effects of reactive oxygen species (including those produced during the course of an immune response) on lymphocytes in vivo, and so help to maintain their functional capacity.

Keywords

Vitamin CVitamin EAntioxidantsDNA damage
Type
Research Article
Information
British Journal of Nutrition , Volume 84 , Issue 2 , August 2000 , pp. 195 - 202
Copyright
Copyright © The Nutrition Society 2000

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