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Effects of dietary fats (fish, olive and high-oleic-acid sunflower oils) on lipid composition and antioxidant enzymes in rat liver

Published online by Cambridge University Press:  09 March 2007

Valentina Ruiz-Gutiérrez ,
Alonso Pérez-Espinosa ,
Carmen María Vázquez  and
Consuelo Santa-María
Valentina Ruiz-Gutiérrez
Affiliation:
Instituto de la Grasa y sus Derivados, CSIC, apdo 1078, Sevilla, Spain
Alonso Pérez-Espinosa
Affiliation:
Instituto de la Grasa y sus Derivados, CSIC, apdo 1078, Sevilla, Spain
Carmen María Vázquez
Affiliation:
Departamento Fisiología y Biología Animal, Facultad de Farmacia, c/ Professor García González s/n, 41012, Sevilla, Spain
Consuelo Santa-María*
Affiliation:
Departamento de Bioquímica, Bromatología y Toxicología, Facultad de Farmacia, c/ Professor García González s/n, 41012, Sevilla, Spain
*
*Corresponding author: Dr Consuelo Santa-María, fax +34 95 423 3765, email csm@cica.es
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Abstract

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The effects of two oleic-acid-rich diets (containing olive oil, OO, and high-oleic-acid sunflower oil, HOSO) on plasma and liver lipid composition detoxification enzyme activities, were compared with those of a fish-oil (FO) diet and a control diet. Compared with the control diet, plasma and hepatic total triacylglycerol concentrations were increased in the animals fed on the HOSO and OO diets and decreased in those fed on the FO diet. The animals fed on FO showed the highest level of cholesterol in the liver and had lower plasma cholesterol concentrations when compared with those fed on the two oleic-acid-rich diets. In comparison with the animals fed on the diets enriched in oleic acid, the FO group showed higher hepatic levels of polyunsaturated fatty acids of the n-3 series and lower levels of fatty acids of the n-6 series. Livers of FO-fed rats, compared with those of OO- and HOSO-fed rats showed: (1) significantly higher activities of catalase (EC 1.11.1.6) glutathione peroxidase (EC 1.11.1.9) and Cu/Zn superoxide dismutase (EC 1.15.1.1); (2) no differences in the NADPH-cytochrome c reductase (EC 1.6.99.3) activity. The HOSO diet had a similar effect on liver antioxidant enzyme activities as the OO diet. In conclusion, it appears that changes in the liver fatty acid composition due mainly to n-3 lipids may enhance the efficiency of the antioxidant defence system. The two monounsaturated fatty acids oils studied (OO and HOSO), with the same high content of oleic acid but different contents of natural antioxidants, had similar effects on the antioxidant enzyme activities measured.

Keywords

Fatty acidsAntioxidant enzymesOleic acid
Type
Research Article
Information
British Journal of Nutrition , Volume 82 , Issue 3 , September 1999 , pp. 233 - 241
Copyright
Copyright © The Nutrition Society 1999

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