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The effects of energy source and tryptophan on the rate of protein synthesis and on hormones of the entero-insular axis in the piglet

Published online by Cambridge University Press:  09 March 2007

A. A. Ponter ,
N. O. Cortamira ,
B. Seve ,
D. N. Salter  and
L. M. Morgan
A. A. Ponter
Affiliation:
Institute of Grassland and Environmental Research, Church Lane, Shinfield, Reading, RG2 9AQ
N. O. Cortamira
Affiliation:
Institute of Grassland and Environmental Research, Church Lane, Shinfield, Reading, RG2 9AQ
B. Seve
Affiliation:
I.N.R.A., Station de Recherches Porcines, Saint-Gilles, 35590 L'Hermitage, France
D. N. Salter
Affiliation:
Institute of Grassland and Environmental Research, Church Lane, Shinfield, Reading, RG2 9AQ
L. M. Morgan
Affiliation:
School of Biological Sciences, University of Surrey, Guildford, Surrey, GU2 5XH
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Abstract

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The present experiment was designed to study the influence of dietary energy source (fat or carbohydrate) and tryptophan (TRP) on protein synthesis and plasma insulin concentrations in the piglet. Six dietary regimens, based on either a high-fat (F) or a high-carbohydrate (C) diet with three levels of TRP (deficient, 1; adequate, 2; excess, 3), were used. Fractional protein synthesis rate (ks; % per d) was measured in the liver, Longissimus dorsi (LD), Semitendinosus (ST), skin, femur, brain, pancreas, stomach, mucosa of the duodenum and jejunum, and the whole body, using a‘flooding dose’of 3H-phenylalanine. Mean integrated insulin, gastric inhibitory polypeptide (GIP) and glucose concentrations were higher after the C diets compared with the F diets, TRP supplementation globally augmented ks linearly in the liver, ST, skin and whole body, while it had quadratic effects in the LD (ks highest in the TRP-adequate diet groups) and jejunal mucosa (ks lowest in the TRP-adequate diet groups). Pancreatic ks was increased by TRP addition up to a plateau. ks was highest after the F diets in the digestive tissues while in the skin and LD ks was highest after the C diets. Fasting concentrations of gluconeogenic amino acids were lower (and urea higher) with the F than the C diets, suggesting their use as precursors for glucose synthesis. In conclusion, we have confirmed the depressive effects of TRP deficiency on ks, RNA activity and growth. We could not establish a relationship between plasma insulin and muscle ks. This may be related to the way in which we manipulated plasma insulin concentrations.

Keywords

Protein synthesisInsulinTryptophanPiglets
Type
Response of protein synthesis and hormonal status to energy and tryptophan intake
Information
British Journal of Nutrition , Volume 71 , Issue 5 , May 1994 , pp. 661 - 674
Copyright
Copyright © The Nutrition Society 1994

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