Hostname: page-component-78c5997874-s2hrs Total loading time: 0 Render date: 2024-11-13T11:28:29.685Z Has data issue: false hasContentIssue false
  • English
  • Français

The effects of fasting on plasma corticosterone kinetics in rats

Published online by Cambridge University Press:  09 March 2007

C. J. H. Woodward ,
G. R. Hervey ,
R. E. Oakey  and
E. M. Whitaker
C. J. H. Woodward
Affiliation:
Department of Physiology, Department of Chemical Pathology, University of Leeds, Leeds LS2 9NQ
G. R. Hervey
Affiliation:
Department of Physiology, Department of Chemical Pathology, University of Leeds, Leeds LS2 9NQ
R. E. Oakey
Affiliation:
Division of Steroid Endocrinology, Department of Chemical Pathology, University of Leeds, Leeds LS2 9NQ
E. M. Whitaker
Affiliation:
Department of Physiology, Department of Chemical Pathology, University of Leeds, Leeds LS2 9NQ
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Plasma corticosterone clearance in anaesthetized rats was measured from the disappearance of radioactivity after a bolus injection of [3H]corticosterone. Mean fractional clearance rates were significantly (P < 0.05) reduced after a 48 h fast, by 32 and 22% for males and females respectively. Plasma corticosterone concentrations were increased by fasting in both sexes. Corticosterone secretion rates, calculated as the product of fractional clearance and plasma corticosterone concentration, did not differ between fed and fasted groups in either sex. The mean activity (U/liver) of the rate-limiting enzyme for corticosterone degradation, hepatic 4,5-dihydrocorticosterone:NADP+ Δ4-oxidoreductase, was significantly reduced by 51 and 78% after fasting in males and females respectively. This was due to changes in both the soluble and microsomal forms of the enzyme. The binding capacity of corticosterone-binding globulin in plasma was significantly reduced by fasting in females (P < 0.001), but was not altered in males. The results suggest that reduced hormone clearance is the dominant cause of fasting hypercorticosteronaemia in the rat.

Keywords

FastingCorticosterone metabolismRat
Type
Hormonal Effects of Dietary Deprivation
Information
British Journal of Nutrition , Volume 66 , Issue 1 , July 1991 , pp. 117 - 127
Copyright
Copyright © The Nutrition Society 1991

References

REFERENCES

Baginski, E. S., Foa, P. P. & Zak, B. (1974). Glucose-6-phosphatase. In Methods of Enzymatic Analysis, 2nd English ed., pp. 876–880 [Bergmeyer, H. U., editor]. Basel: Verlag Chemie.CrossRefGoogle Scholar
Ballard, P. L. (1979). Delivery and transport of glucocorticoids to target cells. In Glucocorticoid Hormone Action, pp. 25–48 [Baxter, J. D. and Rousseau, G.G., editors]. Berlin: Springer-Verlag.Google Scholar
Beer, S. F., Bircham, P. M. M., Bloom, S. R., Clark, P. M., Hales, C. N., Hughes, C. M., Jones, C. T., Marsh, D. R., Raggatt, P. R. & Findlay, A. L. R. (1989). The effect of a 72-h fast on plasma levels of pituitary, adrenal, thyroid, pancreatic and gastrointestinal hormones in healthy men and women. Journal of Endocrinology 120, 337–350.CrossRefGoogle ScholarPubMed
Carsia, R. V., Weber, H. & Lauterio, T. J. (1988). Protein malnutrition in the domestic fowl induces alterations in adrenocortical function. Endocrinology 122, 673–680.CrossRefGoogle ScholarPubMed
Chamness, G. C. & McGuire, W. L. (1975). Scatchard plots: common errors in correction and interpretation. Steroids 26, 538–542.Google Scholar
Chowers, I., Einst, R. & Feldman, S. (1969). Effects of starvation on levels of corticotrophin releasing factor, corticotrophin and plasma corticosterone in rats. Acta Endocrinologica 61, 687–694.Google Scholar
Dawson, R. M. C., Elliott, D. C., Elliott, W. H. & Jones, K. M. (1969). Data for Biochemical Research, 2nd ed., p. 619. London: Oxford University Press.Google Scholar
Dorfman, R. I. & Ungar, F. (1965). Metabolism of Steroid Hormones, 1st ed., pp. 406–409. New York: Academic Press.Google Scholar
Gala, R. R. & Westphal, U. (1965). Corticosteroid-binding globulin in the rat: studies on the sex difference. Endocrinology 77, 841–851.CrossRefGoogle ScholarPubMed
Glenister, D. W. & Yates, F. E. (1961). Sex differences in the rate of disappearance of corticosterone-4-C14 from plasma of intact rats. Endocrinology 68, 747–758.CrossRefGoogle ScholarPubMed
Goldstein, S. & Phillips, L. S. (1989). Nutrition and somatomedin: nutritionally regulated release of somatomedins and somatomedin inhibitors from perfused livers in rats. Metabolism 38, 745–752.CrossRefGoogle ScholarPubMed
Herbst, A. L., Yates, F. E., Glenister, D. W. & Urquhart, J. (1960). Variations in hepatic inactivation of corticosterone with changes in food intake: an explanation of impaired corticosteroid metabolism following noxious stimuli. Endocrinology 67, 222–238.CrossRefGoogle ScholarPubMed
Jansson, J.-O., Oscarsson, J., Mode, A. & Ritzen, E. M. (1989). Plasma growth hormone pattern and androgens influence the levels of corticosteroid-binding globulin in rat serum. Journal of Endocrinology 122, 725–732.CrossRefGoogle ScholarPubMed
Kitay, J. I. (1961). Sex differences in adrenal cortical secretion in the rat. Endocrinology 68, 818–824.Google Scholar
Leeper, L. L., Schroeder, R. & Henning, S. J. (1988). Kinetics of circulating corticosterone in infant rats. Pediatric Research 24, 595–599.CrossRefGoogle ScholarPubMed
Lindberg, M., Shire, J. G. M., Doering, C. H., Kessler, S. & Clayton, R. B. (1972). Reductive metabolism of corticosterone in mice: differences in NADPH requirements of liver homogenates of males of two inbred strains. Endocrinology 90, 81–92.CrossRefGoogle ScholarPubMed
Martin, C. E., Cake, M. H., Hartmann, P. E. & Cook, I. F. (1977). Relationship between foetal corticosteroids, maternal progesterone and parturition in the rat. Acta Endocrinologica 84, 167–176.Google Scholar
Munck, A., Guyre, P. M. & Holbrook, N. J. (1984). Physiological functions of glucocorticoids in stress and their relation to pharmacological actions. Endocrine Reviews 5, 25–44.Google Scholar
Norman, A. W. & Litwack, G. (1987). Hormones. New York: Academic Press.Google ScholarPubMed
Snedecor, G. W. & Cochran, W. G. (1978). Statistical Methods, 6th ed., p. 324. Ames, Iowa: Iowa State University Press.Google Scholar
Stewart, J., Meaney, M. J., Aitken, D., Jensen, L. & Kalant, N. (1988). The effects of acute and life-long food restriction on basal and stress-induced serum corticosterone levels in young and aged rats. Endocrinology 123, 1934–1941.CrossRefGoogle Scholar
Tait, J. F. (1963). The use of isotopic steroids for the measurement of production rates in vivo. Journal of Clinical Endocrinology and Metabolism 23, 1285–1297.CrossRefGoogle ScholarPubMed
Tait, J. F. & Burstein, S. (1964). In vivo studies of steroid dynamics in man. In The Hormones, vol. 5, pp. 441–557 [incus, G. P., Thimann, K. V and Astwood, E. B., editors]. New York: Academic Press.Google Scholar
Tischler, M. E., Henriksen, E. J. & Cook, P. H. (1988). Role of glucocorticoids in increased muscle glutamine production in starvation. Muscle and Nerve 11, 752–756.CrossRefGoogle ScholarPubMed
Tomkins, G. M. (1956). Enzymatic mechanisms of hormone metabolism. 1. Oxidation-reduction of the steroid nucleus. Recent Progress in Hormone Research 12, 125–133.Google Scholar
Ulrich, F. & Long, C. N. H. (1956). Effects of stress on serum C14 levels in rats following administration of hydrocortisone-4-C14 and corticosterone-4-C14. Endocrinology 59, 170–180.CrossRefGoogle Scholar
Waterlow, J. C., Garlick, P. J. & Millward, D. J. (1978). Protein Turnover in Mammalian Tissues and in the Whole Body. Amsterdam: Elsevier/North Holland Biomedical Press.Google Scholar
Waynforth, H. B. (1980). Experimental and Surgical Technique in the Rat. London: Academic Press.Google Scholar
Weber, G. & Cantero, A. (1954). Glucose-6-phosphatase studies in fasting. Science 120, 851–852.Google Scholar
White, B. D., Corll, C. B. & Porter, J. R. (1989). The metabolic clearance rate of corticosterone in lean and obese male Zucker rats. Metabolism 38, 530–536.CrossRefGoogle ScholarPubMed
Woodward, C. J. H., Hervey, G. R., Oakey, R. E. & Whitaker, E. M. (1990). The effect of fasting on plasma corticosterone kinetics in the anaesthetised rat. Proceedings of the Nutrition Society 49, 79A.Google Scholar
Yates, F. E., Herbst, A. L. & Urquhart, J. (1958). Sex difference in rate of ring A reduction of Δ4-3-keto-steroids in vitro by rat liver. Endocrinology 63, 887–902.CrossRefGoogle Scholar