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Effects of intestinal resection, cholecalciferol and ascorbic acid on iron metabolism in rats

Published online by Cambridge University Press:  09 March 2007

S. Hartiti ,
M. Barrionuevo ,
I. López-Aliaga ,
F. Lisbona ,
I. Pallarés ,
M. J. M. Alférez ,
A. E. Gómez-Ayala  and
M. S. Campos
S. Hartiti
Affiliation:
Departamento de Fisiología y Universidad de Granada, E-18071, Granada, Spain
M. Barrionuevo
Affiliation:
Departamento de Fisiología y Universidad de Granada, E-18071, Granada, Spain Instituto de Nutrición y Tecnologia de Alimentos, Universidad de Granada, E-18071, Granada, Spain
I. López-Aliaga
Affiliation:
Departamento de Fisiología y Universidad de Granada, E-18071, Granada, Spain
F. Lisbona
Affiliation:
Departamento de Fisiología y Universidad de Granada, E-18071, Granada, Spain Instituto de Nutrición y Tecnologia de Alimentos, Universidad de Granada, E-18071, Granada, Spain
I. Pallarés
Affiliation:
Departamento de Fisiología y Universidad de Granada, E-18071, Granada, Spain
M. J. M. Alférez
Affiliation:
Departamento de Fisiología y Universidad de Granada, E-18071, Granada, Spain
A. E. Gómez-Ayala
Affiliation:
Departamento de Fisiología y Universidad de Granada, E-18071, Granada, Spain
M. S. Campos
Affiliation:
Departamento de Fisiología y Universidad de Granada, E-18071, Granada, Spain Instituto de Nutrición y Tecnologia de Alimentos, Universidad de Granada, E-18071, Granada, Spain
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Abstract

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The effect of dietary supplementation with ascorbic acid or cholecalciferol on Fe utilization was studied using the metabolic balance technique, in rats in which 50% of the distal small intestine was removed, or in which the mid small intestine was transected and reanastomosed (controls). Three different diets were used. The first (basal diet) contained (g/kg dry wt): protein (casein+50 mg D, L-methionine/g) 120 and fat (medium-chain triacylglycerols, olive oil and sunflower oil, in equal parts) 40. The other diets were obtained by adding ascorbic acid (150 mg/kg diet) or cholecalciferol (0·4 mg/kg diet) to the basal diet. Apparent digestibility coefficient (ADC) and Fe retention were significantly lower in resected animals than in their respective control groups (transected rats). However, the addition of ascorbic acid or cholecalciferol to the basal diet increased the ADC and Fe retention in both transected and resected rats. Five weeks after surgery, resection also resulted in a reduced concentration of Fe in the sternum, but did not reduce the concentration of haemoglobin or serum Fe total Fe-binding capacity or the concentration of Fe in liver, testes, femur or muscle (longissimus dorsi). Supplementation with ascorbic acid increased serum Fe concentration, while the concentration of Fe in muscle was reduced by supplementation with both ascorbic acid and cholecalciferol. Neither supplementation had any effect on the Fe concentration in other tissues, on haemoglobin concentration or plasma total Fe-binding capacity. Thus, supplementation with ascorbic acid or with cholecalciferol increased Fe absorption and reduced the concentration of Fe in muscle.

Keywords

Resected ratsIronAscorbic acidCholecalciferol
Type
Intestinal resection and iron metabolism
Information
British Journal of Nutrition , Volume 73 , Issue 6 , June 1995 , pp. 871 - 880
Copyright
Copyright © The Nutrition Society 1995

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