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Effects of the bacterial status of rats on the changes in some liver cytochrome P450 (EC 1.14.14.1) apoproteins consequent to a glucosinolate-rich diet

Published online by Cambridge University Press:  09 March 2007

L. Nugon-Baudon ,
S. Rabot ,
J.-P. Flinois ,
S. Lory  and
Ph. Beaune
L. Nugon-Baudon*
Affiliation:
INRA, Unité d'Ecologie et de Physiologie du Système Digestif, Centre de Recherches de Jouy-en-Josas, F-78352 Jouy-en-Josas Cedex, France
S. Rabot
Affiliation:
INRA, Unité d'Ecologie et de Physiologie du Système Digestif, Centre de Recherches de Jouy-en-Josas, F-78352 Jouy-en-Josas Cedex, France
J.-P. Flinois
Affiliation:
INSERM, U75, Centre Hospitalier Universitaire Necker, 156 rue de Vaugirard, F-75730 Paris Cedex 15, France
S. Lory
Affiliation:
INRA, Unité d'Ecologie et de Physiologie du Système Digestif, Centre de Recherches de Jouy-en-Josas, F-78352 Jouy-en-Josas Cedex, France
Ph. Beaune
Affiliation:
INSERM, U75, Centre Hospitalier Universitaire Necker, 156 rue de Vaugirard, F-75730 Paris Cedex 15, France
*
*Corresponding author: Dr Lionelle Nugon-Baudon, fax +33 1 34 65 24 62.
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Abstract

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The aim of the present work was to investigate the influence of the intestinal microflora on the changes in hepatic cytochrome P450 apoproteins induced by dietary glucosinolates. Ten rats harbouring a conventional digestive microflora were offered either a diet containing 390g myrosinase-free rapeseed meal/kg (n 5) or a control diet devoid of glucosinolates (n 5). A similar trial was performed using germ-free rats. After 4 weeks of exposure to the dietary regimens, animals were slaughtered and their livers removed for preparation of microsomes and analysis of cytochrome P450 (EC 1.14.14.1). The glucosinolate-rich diet decreased the concentration of total cytochrome P450 in conventional rats only (-34%). The bacterial status did not modify the concentration of apoproteins CYP1A2 and CYP2B1/B2, but greatly decreased the concentration of the male constitutive isoform CYP2C11 (-53 and -45% respectively in conventional and germ-free rats). Germ-free rats fed on the glucosinolate-rich diet had a greater concentration of CYP3A (+139%) and a lower concentration of CYP2E1 (-32%) than their counterparts fed on the control diet. However, these differences were absent in conventional animals. On the whole, the influence of the intestinal microflora on the changes in hepatic cytochrome P450 due to the consumption of cruciferous vegetables is very complex and obviously involves different mechanisms according to the apoprotein.

Keywords

GnotobiologyXenobiotic metabolizing enzymesRapeseed
Type
Short communication
Information
British Journal of Nutrition , Volume 80 , Issue 3 , September 1998 , pp. 231 - 234
Copyright
Copyright © The Nutrition Society 1998

References

Aspry, KE & Bjeldanes, LF (1983) Effects of dietary broccoli and butylated hydroanisole on liver-mediated metabolism of benzo[a]pylene. Food and Chemical Toxicology 21, 133–142.CrossRefGoogle Scholar
Beaune, Ph, Flinois, JP, Kiffel, L, Kremers, P & Leroux, JP (1985) Purification of new cytochrome P450 from human liver micro-somes. Biochimica et Biophysica Acta 840, 364–370.CrossRefGoogle Scholar
Brown, BL, Allis, JW, Simmons, JE & House, DE (1995) Fasting for less than 24h induces cytochrome P450 2E1 and 2B1/2 activities in rats. Toxicology Letters 81, 39–44.CrossRefGoogle Scholar
de Waziers, I, Cugnenc, PH, Yang, CS, Leroux, JP & Beaune, Ph (1990) Cytochrome P-450 isoenzymes, epoxide hydrolase and glutathione transferases in rat and human hepatic and extra-hepatic tissues. Journal of Pharmacology and Experimental Therapeutics 253, 387–394.Google Scholar
Duncan, AJ & Milne, JA (1989) Glucosinolates. Aspects of Applied Biology 19, 75–92.Google Scholar
Lowry, OH, Rosebrough, NJ, Farr, AL & Randall, RJ (1951) Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–275.CrossRefGoogle ScholarPubMed
McDanell, RE, McLean, AEM, Hanley, AB, Heaney, RK & Fenwick, GR (1988) Chemical and biological properties of indole glucosinolates (glucobrassicins): a review. Food and Chemical Toxicology 26, 59–70.CrossRefGoogle ScholarPubMed
Nelson, DR, Koymans, L, Kamataki, T, Stegeman, JJ, Feyereisen, R, Waxman, DJ, Waterman, MR, Gotoh, O, Coon, MJ, Estabrook, RW, Gunsalus, IC & Nebert, DW (1996) P450 superfamily: update on new sequences, gene mapping, accession numbers and nomenclature. Pharmacogenetics 6, 1–42.CrossRefGoogle ScholarPubMed
Nugon-Baudon, L & Rabot, S (1994) Glucosinolates and glucosinolate derivatives: implications for protection against chemical carcinogenesis. Nutrition Research Reviews 7, 205–231.CrossRefGoogle ScholarPubMed
Nugon-Baudon, L, Rabot, S, Szylit, O & Raibaud, P (1990) Glucosinolates toxicity in growing rats: interactions with the hepatic detoxification system. Xenobiotica 20, 223–230.CrossRefGoogle ScholarPubMed
Nugon-Baudon, L, Szylit, O & Raibaud, P (1988) Production of toxic glucosinolate derivatives from rapeseed meal by intestinal microflora of rat and chicken. Journal of the Science of Food and Agriculture 43, 299–308.CrossRefGoogle Scholar
Omura, T & Sato, R (1964) The carbon monoxide-binding pigment of liver microsomes. I. Evidence for its hemoprotein nature. Journal of Biological Chemistry 239, 2370–2378.CrossRefGoogle ScholarPubMed
Rabot, S, Nugon-Baudon, L & Szylit, O (1993) Alterations of the hepatic xenobiotic-metabolizing enzymes by a glucosinolate-rich diet in germ-free rats: influence of a pre-induction with phenobarbital. British Journal of Nutrition 70, 347–354.CrossRefGoogle ScholarPubMed
Roland, N, Nugon-Baudon, L, Flinois, J-P & Beaune, Ph (1994) Hepatic and intestinal cytochrome P-450, glutathione-S-transferase and UDP-glucuronyltransferase are affected by six types of dietary fiber in rats inoculated with human whole fecal flora. Journal of Nutrition 124, 1581–1587.CrossRefGoogle Scholar
Roland, N, Rabot, S & Nugon-Baudon, L (1996) Modulation of the biological effects of glucosinolates by inulin and oat fibre in gnotobiotic rats inoculated with a human whole faecal flora. Food and Chemical Toxicology 34, 671–677.CrossRefGoogle ScholarPubMed
Ryan, D, Lu, AYH & Levin, W (1978) Purification of cytochrome P-450 and P-448 from rat liver microsomes. In Methods in Enzymology, Vol. 52, pp. 113–123 [Fleisher, S and Packer, L, editors]. New York: Academic Press.Google Scholar
Sohn, OS & Fiala, ES (1995) Effects of dietary restriction and fasting on selected rat liver enzymes of xenobiotic metabolism and on AOM-induced DNA guanine methylation in rat liver and colon. Nutrition Cancer 23, 13–22.CrossRefGoogle ScholarPubMed
Wortelboer, HM, de Kruif, CA, van Iersel, AAJ, Noordhoek, J, Blaauboer, BJ, van Bladeren, PJ & Falke, HE (1992) Effects of cooked Brussels sprouts on cytochrome P-450 profile and phase II enzymes in liver and small intestinal mucosa of the rat. Food and Chemical Toxicology 30, 7–27.CrossRefGoogle ScholarPubMed