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Eicosapentaenoic acid and 3,10 dithia stearic acid inhibit the desaturation of trans-vaccenic acid into cis-9, trans-11-conjugated linoleic acid through different pathways in Caco-2 and T84 cells

Published online by Cambridge University Press:  08 March 2007

Renaville Bénédicte ,
Anne Mullen ,
Fiona Moloney ,
Yvan Larondelle ,
Yves-Jacques Schneider  and
Helen M. Roche
Renaville Bénédicte
Affiliation:
Laboratoire de Biochimie cellulaire, Institut des Sciences de la Vie, Université catholique de Louvain, Louvain-la-Neuve, Belgium Unité de Biochimie de la Nutrition, Institut des Sciences de la Vie, Université catholique de Louvain, Louvain-la-Neuve, Belgium Fisiologia Veterinaria e Nutrizione, Dept Science degli Alimenti, Università degli Studi di Udine, Italy
Anne Mullen
Affiliation:
Nutrigenomics Research Group, Department of Clinical Medicine, Trinity College Dublin, St James's Hospital, James's Street, Dublin 8, Republic of Ireland
Fiona Moloney
Affiliation:
Nutrigenomics Research Group, Department of Clinical Medicine, Trinity College Dublin, St James's Hospital, James's Street, Dublin 8, Republic of Ireland
Yvan Larondelle
Affiliation:
Laboratoire de Biochimie cellulaire, Institut des Sciences de la Vie, Université catholique de Louvain, Louvain-la-Neuve, Belgium
Yves-Jacques Schneider
Affiliation:
Unité de Biochimie de la Nutrition, Institut des Sciences de la Vie, Université catholique de Louvain, Louvain-la-Neuve, Belgium
Helen M. Roche*
Affiliation:
Nutrigenomics Research Group, Department of Clinical Medicine, Trinity College Dublin, St James's Hospital, James's Street, Dublin 8, Republic of Ireland
*
*Corresponding author: Dr Helen Roche, fax +353 1 4542043, email hmroche@tcd.ie
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Abstract

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Stearoyl-CoA desaturase (SCD) is a key enzyme that determines the composition and metabolic fate of ingested fatty acids, in particular the conversion of trans-vaccenic acid (TVA) to conjugated linoleic acid (CLA). The present study addressed the hypothesis that intestinal TVA absorption and biotransformation into CLA can be modulated by EPA and 3,10-dithia stearic acid (DSA) via altered SCD mRNA levels and desaturation indices (cis-9, trans-11-CLA:TVA and oleic acid:stearic acid ratios) in Caco-2 and T84 cells, two well-established in vitro models of the human intestinal epithelium. The study determined the effect of acute (3h with 0·3mm-EPA or 0·3mm-DSA) and acute-on-chronic (1 week with 0·03mm-EPA or -DSA, followed by respectively, 0·3mm-EPA or -DSA for 3h) treatments. In both cell lines, acute EPA treatment did not alter SCD desaturation indices, whereas the acute-on-chronic treatment affected these surrogate markers of SCD activity. This was associated with reduced sterol regulatory-element binding protein-1c and SCD mRNA levels. In contrast, acute and acute-on-chronic DSA treatments significantly reduced SCD desaturation indices without affecting SCD mRNA levels in Caco-2 cells. The present study on intestinal cells shows that the conversion rate of TVA to c9, t11-CLA is affected by other fatty acids present in the diet such as EPA, confirming previous observations in hepatic and mammary cell models.

Keywords

Stearoyl-CoA desaturaseTrans fatty acidsConjugated linoleic acid
Type
Research Article
Information
British Journal of Nutrition , Volume 95 , Issue 4 , April 2006 , pp. 688 - 695
Copyright
Copyright © The Nutrition Society 2006

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