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Endogenous amino acid flow in the avian ileum: quantification using three techniques

Published online by Cambridge University Press:  09 March 2007

V. Ravindran ,
L. I. Hew  and
G. Ravindran
V. Ravindran*
Affiliation:
Department of Animal Science, The University of Sydney, Camden, NSW 2570, Australia
L. I. Hew
Affiliation:
Department of Animal Science, The University of Sydney, Camden, NSW 2570, Australia
G. Ravindran
Affiliation:
Department of Animal Science, The University of Sydney, Camden, NSW 2570, Australia
*
*Corresponding author: fax +64 6 350 5685, email V.Ravindran@massay.ac.nz
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Abstract

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The aim of the present study was to compare the protein-free diet, guanidinated casein (GuC) and enzyme hydrolysed casein (EHC) methods for the quantification of endogenous amino acid (AA) flow in the avian ileum. Growing broiler chickens (5 weeks old) were used. All three assay diets were based on dextrose, and in the GuC and EHC diets GuC or EHC were the sole source of N. Endogenous AA flows determined with the use of protein-free diet were considerably lower (P>0·05) than those determined by the GuC and EHC methods. The total endogenous AA flows determined by the GuC and EHC methods were almost 3-fold greater (P>0·05) than those determined by the protein-free diet. The endogenous AA values obtained from GuC and EHC methods were similar (P<0·05), except for the flow of arginine, which was lower (P>0·05) in the EHC method. Glutamic acid, aspartic acid, threonine and glycine were the predominant endogenous AA present in digesta from the distal ileum. The contents of methionine, histidine and cystine were lower compared with other AA. The method of determination had no effect on the AA composition of endogenous protein, except for threonine, glutamic acid, lysine, arginine and cystine. The concentrations of threonine and arginine were lower (P>0·05) and that of lysine was higher (P>0·05) with the EHC method compared with the other two methods. The concentration of glutamic acid was greater (P0·05) and that of cystine was lower (P>0·05) in the EHC and GuC methods compared with the protein-free diet method. The results showed that the ileal endogenous flows of N and AA are markedly enhanced by the presence of protein and peptides, above those determined following feeding of a protein-free diet. It is concluded that the use of EHC and GuC methods enables the measurement of ileal endogenous losses in chickens under normal physiological conditions.

Keywords

Ileal endogenous amino acid flowEnzyme hydrolysed caseinGuanidinated caseinProtein-free dietChickens
Type
Research Article
Information
British Journal of Nutrition , Volume 92 , Issue 2 , August 2004 , pp. 217 - 223
Copyright
Copyright © The Nutrition Society 2004

References

Angkanaporn, K, Choct, M, Bryden, WL, Annison, EF & Annison, GEffect of wheat pentosans on endogenous amino acid losses in chickens. J Sci Food Agric (1994) 66, 399 – 404.CrossRefGoogle Scholar
Angkanaporn, K, Ravindran, V, Mollah, Y & Bryden, WLHomoarginine influences voluntary feed intake, tissue basic amino acid concentrations and arginase activity in chickens. J Nutr (1997) 127, 1128 – 1136.CrossRefGoogle ScholarPubMed
Bryden, WL, Angkanaporn, K, Ravindran, V, Imbeah, M & Annison, EFUse of homoarginine technique to determine endogenous amino acid losses in poultry and pigs. In Protein Metabolism and Nutrition, European Association of Animal Production Publication no. 81, pp. 319–323 [Nunes, AF, Portugal, AV, Costa, JP and Ribeiro, JP, editor]. Vale de Saltoverm: Estacao Zootecnica Nacional. (1996)Google Scholar
Butts, CA, Moughan, PJ & Smith, WCEndogenous amino acid flow at the terminal ileum of the rat determined under conditions of peptide alimentation. J Sci Food Agric (1991) 55, 175 – 187.CrossRefGoogle Scholar
Butts, CA, Moughan, PJ, Smith, WC, Reynolds, GW & Garrick, DJThe effect of food dry matter intake on endogenous endogenous ileal amino acid excretion determined under peptide alimentation in the 50 kg liveweight pig. J Sci Food Agric (1993) 62, 235 – 243.CrossRefGoogle Scholar
Caine, WR, Sauer, WC, Verstegen, WA, Tamminga, S, Li, S & Schulze, HGuanidinated protein test meals with higher concentration of soybean trypsin inhibitors increase ileal recoveries of endogenous amino acids in pigs. J Nutr (1998) 128, 598 – 605.CrossRefGoogle ScholarPubMed
Cremers, S, Pallauf, J, Hohler, D, Lemme, A & Pack, MEndogenous amino acid flow at the distal ileum of growing broiler chickens fed different diets. In Proceedings of the 13th European Symposium on Poultry Nutrition, pp. 254 – 255Blankenburg: World’s Poultry Science Association. (2001)Google Scholar
Darragh, AJ, Moughan, PJ & Smith, WCThe effect of amino acid and peptide alimentation on the determination of endogenous amino acid flow at the terminal ileum of the rat. J Sci Food Agric (1990) 51, 47 – 56.CrossRefGoogle Scholar
de Lange, CFM, Sauer, WC, Mosenthin, R & Souffrant, WBThe effect of feeding different protein-free diets on the recovery and amino acid composition of endogenous protein collected from the distal ileum and feces of pigs. J Anim Sci (1989) 67, 746 – 754.CrossRefGoogle ScholarPubMed
Donkoh, A, Moughan, PJ & Morel, PCHComparison of methods to determine the endogenous amino acid flow at the terminal ileum of the growing rat. J Sci Food Agric (1995) 67, 359 – 366.CrossRefGoogle Scholar
Hagemeister, H, & Erbersdobler, HChemical labelling of dietary protein by transformation of lysine to homoarginine: a new technique to follow intestinal digestion and absorption. Proc Nutr Soc (1985) 44, 133A.Google Scholar
Hess, V & Seve, BEffects of body weight and feed intake level on basal ileal endogenous losses in growing pigs. J Anim Sci (1999) 77, 3281 – 3288.CrossRefGoogle ScholarPubMed
Hodgkinson, SM, Moughan, PJ, Reynolds, GW & James, KACThe effect of dietary peptide concentration on endogenous ileal amino acid loss in the growing pig. Br J Nutr (2000) 83, 421 – 430.Google ScholarPubMed
Hodgkinson, SM, Souffrant, WB & Moughan, PJComparison of three methods for determining endogenous ileal protein flow in the growing pig. In Manipulating Pig Production VII, p. 270 [Cranwell, PD, editor]. Canberra: Australian Pig Science Association. (1999)Google Scholar
Imbeah, M, Angkanaporn, K, Ravindran, V & Bryden, WLInvestigations on the guanidination of lysine in proteins. J Sci Food Agric (1996) 72, 213 – 218.3.0.CO;2-N>CrossRefGoogle Scholar
James, KAC, Butts, CA, Koolaard, JP, Donaldson, HE, Scott, MF & Moughan, PJThe effect of food dry matter intake on the flow of amino acids at the terminal ileum for rats fed an enzyme-hydrolysed casein-based diet. J Sci Food Agric (2002) 82, 1128 – 1135.CrossRefGoogle Scholar
Jansman, AJM, Smink, W, van Leeuwen, P & Rademacher, MEvaluation through literature data of the amount and amino acid composition of basal endogenous crude protein at the terminal ileum of pigs. Anim Feed Sci Technol (2002) 98, 49 – 60.CrossRefGoogle Scholar
Leterme, P, Monmart, T, Thewis, A & Morandi, PEffect of oral and parenteral N nutrition vs N-free nutrition on the endogenous amino acid flow at the ileum of the pig. J Sci Food Agric (1996) 71, 265 – 271.3.0.CO;2-F>CrossRefGoogle Scholar
Lien, KA, Sauer, WA & Fenton, MMucin output in ileal digesta of pigs fed a protein-free diet. J Anim Sci (1997) 72, 1737 – 1743.Google Scholar
Moore, SOn the determination of cystine as cysteic acid. J Biol Chem (1963) 238, 235 – 237.CrossRefGoogle Scholar
Moughan, PJ, Darragh, AJ, Smith, WC & Butts, CAPerchloric and trichloraacetic acids as precipitants of protein in endogenous ileal digesta from the rat. J Sci Food Agric (1990) 52, 13 – 21.CrossRefGoogle Scholar
Moughan, PJ & Schuttert, GComposition of nitrogen-containing fractions in digesta from the distal ileum of pigs fed a protein-free diet. J Nutr (1991) 121, 1570 – 1574.CrossRefGoogle ScholarPubMed
Moughan, PJ, Schuttert, G & Leenaars, MEndogenous amino acid flow in the stomach and small intestine of the young growing pig. J Sci Food Agric (1992) 60, 437 – 442.CrossRefGoogle Scholar
Moughan, PJ, Souffrant, WB & Hodgkinson, SMPhysiological approaches to determining gut endogenous amino acid flows in the mammal. Arch Anim Nutr (1992) 51, 237 – 252.Google Scholar
Nyachoti, CM, de Lange, CFM, McBride, BW & Schulze, HSignificance of endogenous gut protein losses in the nutrition of growing pigs. Can J Anim Sci (1997) 77, 149 – 163.CrossRefGoogle Scholar
Ozimek, L, Sauer, WC & Ozimek, GEffect of Diet on the Qualitative and Quantitative Adaptation of Exocrine Pancreas Secretions. Feeders Day Report, vol. 63, pp.16 – 20. University of Alberta: Department of Animal Science. (1985)Google Scholar
Pedersen, C & Boisen, SEstablishment of tabulated values for standardized ileal digestibility of crude protein and essential amino acids in common feedstuffs for pigs. Acta Agric Scand A (2002) 52, 121 – 140.Google Scholar
Rademacher, M, Sauer, WC & Jansman, AJMStandardised Ileal Digestibility of Amino Acids in Pigs. Hanua: Feed Additives Division, Degussa-Huls AG. (1999)Google Scholar
Ravindran, G & Bryden, WLDetermination of the tryptophan content of proteins by ion exchange chromatography. Proc Nutr Soc Aust (1996) 20, 105.Google Scholar
Ravindran, G & Bryden, WLAmino acid availability in poultry in vitro and in vivo measurements. Aust J Agric Res (1999) 50, 889 – 908.CrossRefGoogle Scholar
Ravindran, G, Imbeah, M, Angkanaporn, K & Bryden, WLGuanidination of lysine in cottonseed protein. J Agric Food Chem (1996) 44, 1812 – 1815.CrossRefGoogle Scholar
Rutherfurd, SM, Chung, TK & Moughan, PJThe effect of microbial phytase on ileal phosphorus and amino acid digestibility in the broiler chicken. Br Poult Sci (2002) 44, 598 – 606.CrossRefGoogle Scholar
Sibbald, IREstimation of bioavailable amino acids in feedstuffs for poultry and pigs: a review with emphasis on balance experiments. Can J Anim Sci (1987) 67, 221 – 230.CrossRefGoogle Scholar
Siriwan, P, Bryden, WL & Annison, EFUse of guanidinated dietary protein to measure losses of endogenous amino acids in poultry. Br J Nutr (1994) 71, 515 – 529.CrossRefGoogle ScholarPubMed
Siriwan, P, Bryden, WL, Mollah, Y & Annison, EFMeasurement of endogenous amino acid losses in poultry. Br Poult Sci (1993) 34, 939 – 949.CrossRefGoogle ScholarPubMed
Snook, JT & Meyer, JHResponse of digestive enzymes to dietary protein. J Nutr (1964) 82, 409 – 414.CrossRefGoogle ScholarPubMed
Souffrant, WBEndogenous nitrogen losses during digestion in pigs. In Proceedings of the Vth International Symposium on Digestive Physiology in Pigs, pp. 147–166 [Verstegen, MWA, Huisman, L and den Hartog, LA, editor]. Wageningen: PUDOC. (1991)Google Scholar
Sweeney, RAGeneric combustion method for determination of crude protein in feeds: Collaborative study. J Assoc Off Anal Chem (1989) 72, 770 – 774.Google ScholarPubMed
Taverner, MR, Hume, ID & Farrell, DJAvailability to pigs of amino acids in cereal grains. 1. Endogenous levels of amino acids in ileal digesta and faeces of pigs given cereal diets. Br J Nutr (1981) 46, 149 – 158.CrossRefGoogle ScholarPubMed