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The end-product method of measuring whole-body protein turnover: a review of published results and a comparison with those obtained by leucine infusion

Published online by Cambridge University Press:  08 March 2007

S. L. Duggleby  and
J. C. Waterlow
S. L. Duggleby
Affiliation:
MRC Epidemiology Resource Centre, Southampton General Hospital, Southampton 5022 5A, UK
J. C. Waterlow*
Affiliation:
Nutrition Unit, Department of Epidemiology and Population Health, London School of Hygiene & Tropical Medicine, Keppel Street, London C1E 7HT, UK
*
*Corresponding author:
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Abstract

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The present review summarizes the results of all published papers on whole-body protein turnover in man measured by [15N]glycine and the end-product method using both urea and ammonia. It begins with a short account of the underlying assumptions and the justification for the use of [15N]glycine. The results are then compared with those of a large sample of measurements by the ‘gold standard’ precursor method with continuous infusion of [13C]leucine. The pros and cons of the two methods are compared and it is suggested that there is a place for further work by the less invasive end-product method, particularly for population studies of the genetic, environmental and functional determinants of whole-body rates of protein synthesis.

Keywords

Protein turnover[15N]GlycineEnd productLeucine infusion
Type
Review article
Information
British Journal of Nutrition , Volume 94 , Issue 2 , August 2005 , pp. 141 - 153
Copyright
Copyright © The Nutrition Society 2005

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