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Excretion of benzoic acid derivatives in urine of sheep given intraruminal infusions of 3-phenylpropionic and cyclohexanecarboxylic acids

Published online by Cambridge University Press:  24 July 2007

J. H. Pagella ,
X. B. Chen ,
N. A. Macleod ,
E. R. Ørskov  and
P. J. S. Dewey
J. H. Pagella
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB
X. B. Chen
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB
N. A. Macleod
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB
E. R. Ørskov
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB
P. J. S. Dewey
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB
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Abstract

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The quantitative relationship between the urinary excretion of benzoic acid (BA)and the uptake of 3-phenylpropionic (PPA) and cyclohexanecarboxylic (CHCA) acids was assessed.PPA and CHCA are produced in the rumen by microbial fermentation of lignocellulosic feeds and metabolized, after absorption, to BA which is excreted in the urine mainly as its glycine conjugate hippuric acid (HA). Four sheep nourished by intragastric infusions of all nutrients weregiven continuous ruminal infusions of PPA (8,16 or 24 mmol/d) either alone or with CHCA (8 or 16 mmol/d) in a factorial experiment. The treatments were allocated to ten consecutive 6 d periods, with a control being repeated at periods 1, 5 and 10. PPA and CHCA ruminal absorption rates, estimated using the liquid-phase marker Cr-EDTA, were 0·78 (SD 0·29)/h and 0·88 (SD 0·28)/h respectively. For the control, HA excretion was only 0·22 (SD 0·33) mmol/d and free BA was absent. For the other treatments, both HA and free BA were present and HA accounted for 0·85 (SD 0·05) of total BA. The urinary excretion of total BA showed a significant linear correlation (r = 0·997, P<0·001) with the amounts of PPA and CHCA infused. The urinary recovery of infused PPA and CHCA as total BA was 0·79 (SE 0·01). Faecal excretion of BA and its precursors was negligible. Results of this study show that urinary total BA is a potential estimator of the absorption of PPA + CHCA produced in the rumen

Keywords

Benzoic acidHippuric acid3-Phenylpropionic acidCyclohexanecarboxylic acidSheep
Type
Animal Nutrition
Information
British Journal of Nutrition , Volume 77 , Issue 4 , April 1997 , pp. 577 - 592
Copyright
Copyright © The Nutrition Society 1997

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