Hostname: page-component-cd9895bd7-dzt6s Total loading time: 0 Render date: 2024-12-26T07:51:19.015Z Has data issue: false hasContentIssue false
  • English
  • Français

Exogenous and endogenous nitrogen flow rates and level of protein hydrolysis in the human jejunum after [15N]milk and [15N]yoghurt ingestion

Published online by Cambridge University Press:  09 March 2007

Clarie Gaudichon ,
Sylvain Mahé ,
Nils Roos ,
Robert Benamouzig ,
Catherine Luengo ,
Jean-François Huneau ,
Hinrich Sick ,
Christine Bouley ,
Jaques Rautureau  and
Daniel Tome
Clarie Gaudichon
Affiliation:
Unité INRA de Nutrition Humaine et de Physiologie Intestinale, Faculté des Sciences Pharmaceutiques et Biologiques, 4 avenue de l'Observatoire, 75006 Paris, France
Sylvain Mahé
Affiliation:
Unité INRA de Nutrition Humaine et de Physiologie Intestinale, Faculté des Sciences Pharmaceutiques et Biologiques, 4 avenue de l'Observatoire, 75006 Paris, France
Nils Roos
Affiliation:
Institut für Physiologie und Biochemie der Ernährung, Bundesanstalt für Milchforschung, PO Box 6069, D-2300 Kiel 14, Germany
Robert Benamouzig
Affiliation:
Service de Gastroentérologie, Hôpital Avicenne, 125 route de Stalingrad, 93009 Bobigny Cédex, France
Catherine Luengo
Affiliation:
Unité INRA de Nutrition Humaine et de Physiologie Intestinale, Faculté des Sciences Pharmaceutiques et Biologiques, 4 avenue de l'Observatoire, 75006 Paris, France
Jean-François Huneau
Affiliation:
Unité INRA de Nutrition Humaine et de Physiologie Intestinale, Faculté des Sciences Pharmaceutiques et Biologiques, 4 avenue de l'Observatoire, 75006 Paris, France
Hinrich Sick
Affiliation:
Institut für Physiologie und Biochemie der Ernährung, Bundesanstalt für Milchforschung, PO Box 6069, D-2300 Kiel 14, Germany
Christine Bouley
Affiliation:
Groupe Danone, Branche Produits Frais, 15 avenue Galilée, 92350 Le-Plessis-Robinson, France
Jaques Rautureau
Affiliation:
Service de Gastroentérologie, Hôpital Avicenne, 125 route de Stalingrad, 93009 Bobigny Cédex, France
Daniel Tome
Affiliation:
Unité INRA de Nutrition Humaine et de Physiologie Intestinale, Faculté des Sciences Pharmaceutiques et Biologiques, 4 avenue de l'Observatoire, 75006 Paris, France
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Milk and yoghurt proteins were 15N-labelled in order to measure the flow rate of exogenous N during digestion in the human intestine. After fasting overnight, sixteen healthy volunteers, each with a naso-jejunal tube, ingested either [15N]milk (n 7) or [15N]yoghurt (n 9). Jejunal samples were collected every 20 min for 4 h. A significant stimulation of endogenous N secretion was observed during the 20–60 min period after yoghurt ingestion and the 20–40 min period after milk ingestion. The endogenous N flows over a 4 h period did not differ between the groups (44·3 (SEM 6·5) mmol for milk and 63·5 (SEM 5·9) mmol for yoghurt). The flow rates of exogenous N indicated a delayed gastric emptying of the yoghurt N compared with N from milk. The jejunal non-protein N (NPN) flow rate increased significantly after milk and yoghurt ingestion due to an increase in the exogenous NPN flow rate. The NPN fraction of exogenous N ranged between 40 and 80%. The net gastro-jejunal absorption of exogenous N did not differ significantly between milk (56·7 (SEM 8·5)%) and yoghurt (50·9 (SEM 7)%). The high level of exogenous N hydrolysis is in accordance with the good digestibility of milk products. Fermentation modifies only the gastric emptying rate of N and does not affect the level of diet hydrolysis, the endogenous N stimulation or the digestibility rate.

Keywords

Milk proteins15NDigestionHumanJejunum
Type
Digestion of protein from milk and yoghurt
Information
British Journal of Nutrition , Volume 74 , Issue 2 , August 1995 , pp. 251 - 260
Copyright
Copyright © The Nutrition Society 1995

References

REFERENCES

Alpers, D. H. (1987). Digestion and absorption of carbohydrates and proteins. In Physiology of the Gastrointestinal Tract, pp. 1469–1487 [Johnson, L. R. editor]. New York: Raven Press.Google Scholar
Butts, C, Moughan, P. & Wilson, M. (1993). Endogenous amino acid flow at the terminal ileum of adult humans determined following the ingestion of a single protein-free meal. Journal of the Science of Food and Agriculture 61, 439–442.Google Scholar
Corring, T., Juste, C. & Lhoste, E. F. (1989). Nutritional regulation of pancreatic and biliary secretions. Nutrition Research Reviews 2, 161–180.Google Scholar
De Lange, C. F. M., Sauer, W. C, Mosenthin, R. & Souffrant, W. B. (1989). The effect of feeding different protein-free diets on the recovery and amino acid composition of endogenous protein collected from the distal ileum and feces in pigs. Journal of Animal Science 67, 746–754.CrossRefGoogle ScholarPubMed
Gaudichon, C, Laurent, C, Mahé, S., Marks, L., Tomé, D. & Krempf, M. (1994 a). Rate of [15N]leucine incorporation and determination of nitrogenous fractions from gastro-jejunal secretion in fasting humans. Reproduction Nutrition Development 34, 349–359.CrossRefGoogle ScholarPubMed
Gaudichon, C, Roos, N., Mahé, S., Sick, H., Bouley, C. & Tomé, D. (1994 b). Gastric emptying is a major regulatory system of nitrogen absorption kinetics from [15N]-labelled milk and yoghurt in miniature pigs. Journal of Nutrition 124, 1970–1977.Google Scholar
Girard-Globa, A., Bourdel, G. & Lardeux, B. (1980). Regulation of protein synthesis and enzyme accumulation in the rat pancreas by the amount and timing of dietary protein. Journal of Nutrition 110, 1380–1390.CrossRefGoogle ScholarPubMed
Hagemeister, H. & Roos, N. (1991). Comparison of protein digestibility measured by [15N] and homoarginine. In Protein Metabolism and Nutrition. European Association for Animal Production Publication no. 59, pp. 36–38 [Eggum, B. O., Boisen, S., Borsting, C., Danfer, A. and Hvelplund, T., editors]. Foulum, Denmark: National Institute of Animal Science.Google Scholar
Houghton, L. A., Hickson, F. & Read, N. W. (1987). Effect of food consistency on gastric emptying in man. Gut 28, 1584–1588.Google Scholar
Liddle, R. A., Green, G. M., Conrad, C. K. & Williams, J. A. (1986). Proteins but not amino acids, carbohydrate, or fats stimulate CCK secretion in the rat. American Journal of Physiology 251, G243–G248.Google ScholarPubMed
Lurie, B., Novis, B. H., Brom, B., Bank, S. & Marks, I. N. (1973). Pancreatic exocrine responses to test meals of varying compositions in man. American Journal of Digestive Diseases 18, 847–850.CrossRefGoogle ScholarPubMed
Mahé, S., Huneau, J. F., Marteau, P., Thuillier, F. & Tomé, D. (1992). Gastroileal nitrogen and electrolyte movements after bovine milk ingestion in humans. American Journal of Clinical Nutrition 56, 410–416.Google Scholar
Mahé, S., Fauquant, J., Gaudichon, C, Roos, N., Maubois, J. L. & Tomé, D. (1994 a). [15N]labelling and preparation of milk, casein and whey proteins. Lait 74, 307–312.CrossRefGoogle Scholar
Mahé, S., Marteau, P., Huneau, J. F., Thuillier, F. & Tomé, D. (1994 b). Intestinal nitrogen and electrolyte movements following fermented milk ingestion in man. British Journal of Nutrition 71, 169–180.Google Scholar
Mahé, S., Roos, N., Benamouzig, R., Sick, H., Baglieri, A., Huneau, J. F. & Tomé, D. (1994 c). True exogenous and endogenous fractions in the human jejunum after ingestion of small amounts of 15N-labeled casein. Journal of Nutrition 124, 548–555.Google Scholar
Siegel, J. A., Urbain, J. L., Adler, L. P., Charkes, N. D., Maurer, A. H., Krevsky, B., Knight, L. C, Fisher, R. S. & Malmud, L. S. (1988). Biphasic nature of gastric emptying. Gut 29, 85–89.Google Scholar
Simon, O., Zebrowska, T., Bergner, H. & Münchmeyer, R. (1983). Investigations on the pancreatic and stomach secretion in pigs by the means of continuous infustion of 14C-amino acids. Archiv für Tierernährung 33, 9–22.CrossRefGoogle Scholar