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Extracellular signal-regulated kinase 1\2 and protein phosphatase 2A are involved in the antiproliferative activity of conjugated linoleic acid in MCF-7 cells

Published online by Cambridge University Press:  08 March 2007

Antonella Miglietta ,
Francesca Bozzo ,
Ludovica Gabriel ,
Claudia Bocca  and
Rosa Angela Canuto
Antonella Miglietta*
Affiliation:
Department of Experimental Medicine and Oncology, University of Turin, C. Raffaello 30, 10125, Turin, Italy
Francesca Bozzo
Affiliation:
Department of Experimental Medicine and Oncology, University of Turin, C. Raffaello 30, 10125, Turin, Italy
Ludovica Gabriel
Affiliation:
Department of Experimental Medicine and Oncology, University of Turin, C. Raffaello 30, 10125, Turin, Italy
Claudia Bocca
Affiliation:
Department of Experimental Medicine and Oncology, University of Turin, C. Raffaello 30, 10125, Turin, Italy
Rosa Angela Canuto
Affiliation:
Department of Experimental Medicine and Oncology, University of Turin, C. Raffaello 30, 10125, Turin, Italy
*
*Corresponding author: Dr Antonella Miglietta, fax +39 011 6707753, email antonella.miglietta@unito.it
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Abstract

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Conjugated linoleic acid (CLA) has protective properties in breast cancer. Here, we studied the mechanisms underlying the effects of CLA on MCF-7 breast cancer cell proliferation, especially in correlation with the involvement of the extracellular signal-regulated kinase 1/2 (ERK1/2) pathway and protein phosphatase 2A (PP2A). CLA inhibits MCF-7 cell growth in a concentration- and time-dependent manner, without triggering apoptosis. In assessing expression levels of proteins that play obligatory roles in the ERK cascade, we evidenced that CLA down-regulated Raf-1 and decreased levels of phospho-ERK1/2, as well as c-myc expression. Increase in PP2A expression rates were additionally observed after CLA treatment of MCF-7 cells. The above effects, as well as CLA-induced inhibition of cell growth, were reversed by okadaic acid, a specific inhibitor of PP2A. Thus, PP2A likely participates in deactivation of ERK1/2, and its up-regulation may represent a novel mechanism for CLA-induced inhibition of cell proliferation.

Keywords

Conjugated linoleic acidMCF-7 cell lineExtracellular signal-regulated kinase 1/2protein phosphatase 2AProliferation
Type
Research Article
Information
British Journal of Nutrition , Volume 96 , Issue 1 , January 2006 , pp. 22 - 27
Copyright
Copyright © The Nutrition Society 2006

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