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Fish-bone peptide increases calcium solubility and bioavailability in ovariectomised rats

Published online by Cambridge University Press:  08 March 2007

Won-Kyo Jung ,
Bae-Jin Lee  and
Se-Kwon Kim
Won-Kyo Jung
Affiliation:
Department of Chemistry, Pukyong National University, Busan 608-737, Republic of Korea Pukyong National University, Marine Bioprocess Research Center, Busan 608-737, Republic of Korea
Bae-Jin Lee
Affiliation:
Department of Chemistry, Pukyong National University, Busan 608-737, Republic of Korea Marine Bioprocess Co., Ltd., Pukyong National University, Busan 608-737, Republic of Korea
Se-Kwon Kim*
Affiliation:
Department of Chemistry, Pukyong National University, Busan 608-737, Republic of Korea Pukyong National University, Marine Bioprocess Research Center, Busan 608-737, Republic of Korea
*
*Corresponding author: Dr Se-Kwon Kim, fax +82 51 628 8147, email sknkim@pknu.ac.kr
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Abstract

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Fish-bone peptides (FBP) with a high affinity to Ca were isolated using hydroxyapatite affinity chromatography, and FBP II with a high ratio of phosphopeptide was fractionated in the range of molecular weight 5·0–1·0kDa by ultramembrane filtration. In vitro study elucidated that FBP II could inhibit the formation of insoluble Ca salts in neutral pH. In vivo effects of FBP II on Ca bioavailability were further examined in the ovariectomised rat. During the experimental period, Ca retention was increased and loss of bone mineral was decreased by FBP II supplementation in ovariectomised rats. After the low-Ca diet, the FBP II diet, including both normal level of Ca and vitamin D, significantly decreased Ca loss in faeces and increased Ca retention compared with the control diet. The levels of femoral total Ca, bone mineral density, and strength were also significantly increased by the FBP II diet to levels similar to those of the casein phosphopeptide diet group (no difference; P>0·05). In the present study, the results proved the beneficial effects of fish-meal in preventing Ca deficiency due to increased Ca bioavailability by FBP intake

Keywords

Fish-bone peptidesCalcium solubilityOvariectomised ratsCalcium bioavailability
Type
Research Article
Information
British Journal of Nutrition , Volume 95 , Issue 1 , January 2006 , pp. 124 - 128
Copyright
Copyright © The Nutrition Society 2006

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