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Free and esterified fatty acid and cholesterol synthesis in adult malesand its effect on the doubly-labelled water method

Published online by Cambridge University Press:  09 March 2007

P. Haggarty ,
P. Shetty ,
S. Thangam ,
S. Kumar ,
A. Kurpad ,
J. Ashton ,
E. Milne  and
C. Earl
P. Haggarty*
Affiliation:
Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen, AB21 9SB, UK
P. Shetty
Affiliation:
Nutrition Research Centre, St John's Medical College, Bangalore, India London School of Hygiene and Tropical Medicine, 2 Taviton Street, London WC1H 0BT, UK
S. Thangam
Affiliation:
Nutrition Research Centre, St John's Medical College, Bangalore, India
S. Kumar
Affiliation:
Nutrition Research Centre, St John's Medical College, Bangalore, India
A. Kurpad
Affiliation:
Nutrition Research Centre, St John's Medical College, Bangalore, India
J. Ashton
Affiliation:
Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen, AB21 9SB, UK
E. Milne
Affiliation:
Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen, AB21 9SB, UK
C. Earl
Affiliation:
Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen, AB21 9SB, UK
*
*Corresponding author: Dr Paul Haggarty, present address Nutrition and Early Development Laboratory, Department of Obstetrics and Gynaecology, University of Aberdeen, Aberdeen Maternity Hospital, Foresterhill, Aberdeen AB9 2ZD, UK, fax +44 (0)1224 684880, email p.haggarty@abdn.ac.uk
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Abstract

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The purpose of the present study was to estimate whole-body fatty acid and cholesterol synthesis in weight-stable adults and to determine the likely effect on the doubly-labelled water (DLW) method for measuring energy expenditure. Synthesis was measured by 2H incorporation over 14 d in six adult males in approximate energy balance following noradrenaline infusion to maximize mobilization of free fatty acid from adipose tissue. The inter-individual variation in synthesis rates was large and in one subject the proportion of free fatty acid synthesized was ten times that of the mean of the rest of the group; the fasting concentration of esterified fatty acid in this subject was five times that of the rest of the group indicating likely violation of the assumptions underlying the calculation of whole-body synthesis. After 14 d of labelling in the other five subjects, 0·9 (SEM 0·3) % OF THE CIRCULATING FREE FATTY ACID, 9·3 (sem 3·0) % of the esterified fatty acid, 14·6 (sem 2·4) % of the free cholesterol and 28·3 (sem 3·7) % of esterified cholesterol had been synthesized de novo. A high rate of synthesis correlated with a low pre-dose 2H abundance both within and between lipid classes suggesting that natural 2H abundance variations in some lipid classes may be used to determine their metabolic origin. Whole-body synthetic rates were 8 g/d for fatty acid and 0·3–0·5 g/d for cholesterol. These values correspond to very small errors on DLW-derived estimates of CO2 production; -2·5 litres/d for fatty acid and -0·1 to -0·2 litres/d for cholesterol. These results, obtained in subjects typically consuming a diet with a lower fat and cholesterol content that the typical Western diet, suggest that the DLW method is unlikely to be affected by fatty acid and cholesterol synthesis in subjects in energy balance consuming a typical Western diet.

Keywords

DeuteriumDoubly-labelled waterEnergy expenditureLipid synthesis
Type
Research Article
Information
British Journal of Nutrition , Volume 83 , Issue 3 , March 2000 , pp. 227 - 234
Copyright
Copyright © The Nutrition Society 2000

References

Armstrong, DT, Steele, R, Altszuler, N, Dunn, A, Bishop, JS and DeBodo WM (1961) Regulation of plasma free fatty acid turnover.American Journal of Physiology 201, 9–15.CrossRefGoogle ScholarPubMed
Begom, R and Singh, RB (1995) Prevalence of coronary artery disease and its risk factors in the urban population of south and north India.Acta Cardiologica 50, 227–240.Google Scholar
Begom, R and Singh, RB (1997) Association of higher saturated fat intake with higher risk of hypertension in an urban population of Trivandrum in South India.International Journal of Cardiology 58, 63–70.CrossRefGoogle Scholar
Bligh, EG and Dyer, WJ (1959) A rapid method of total lipid extraction and purification.Canadian Journal of Biochemistry and Physiology 37, 911–917.Google Scholar
Department of Health (1991) Dietary Values for Food Energy and Nutrients for the United Kingdom. Report on Health and Social Subjects no. 41. London: H.M. Stationery Office.Google Scholar
Dietschy, JM and Spady, DK (1984) Measurement of rates of cholesterol synthesis using tritiated water.Journal of Lipid Research 25, 1469–1476.CrossRefGoogle ScholarPubMed
Dietschy, JM, Turley, SD and Spady, DK (1993) Role of liver in the maintenance of cholesterol and low density lipoprotein homeostasis in different animal species, including humans.Journal of Lipid Research 34, 1637–1659.CrossRefGoogle ScholarPubMed
Eidinof, ML, Perri, GC, Knoll, JE, Marano, BJ and Arnheim, J (1953) The fractionation of hydrogen isotopes in biological systems.Journal of the American Chemical Society 75, 240–248.Google Scholar
Goodman, DeWS, Noble, RP and Dell, RB (1973) Three-pool model of long-term turnover of plasma cholesterol in man.Journal of Lipid Research 14, 178–188.CrossRefGoogle ScholarPubMed
Haggarty, P (1990) The effect of isotope sequestration on the performance of the heavy water technique for measuring energy expenditure. In The Doubly-labelled Water Method of Measuring Energy Expenditure, pp. 114–146 [Prentice, AM, editor]. Vienna: International Atomic Energy Agency.Google Scholar
Haggarty, P, Franklin, MF, Fuller, MF, MacGaw, BA, Christie, SL, Milne, E, Duncan, G and Smith, JS (1994) Validation of the doubly labeled water method in growing pigs.American Journal of Physiology 267, R1574–R1588.Google ScholarPubMed
Haggarty, P, McGaw, BA, Fuller, MF, Christie, SL and Wong, WW (1990) Water hydrogen incorporation into body fat in growing pigs; its effect on the double and triple labeled water methods.American Journal of Physiology 260, R627–R634.Google Scholar
Haggarty, P, Valencia, ME, McNeill, G, Moya, SY, Pinelli, A, Quihui, L, Davalos, R, Ashton, J, Milne, E and James, WPT (1997) Energy expenditure during heavy work and its interaction with body weight.British Journal of Nutrition 77, 359–373.CrossRefGoogle Scholar
Halliwell, KJ, Fielding, BA, Samra, JS, Humphreys, SM and Frayn, KN (1996) Release of individual fatty acids from human adipose tissue in vitro after an overnight fast.Journal of Lipid Research 37, 1842–1848.CrossRefGoogle Scholar
Hellerstein, MK, Christiansen, M, Kaempfer, S, Kletke, C, Wu, K, Reid, JS, Mulligan, K, Hellerstein, NS and Shackleton, CHL (1991) Measurement of de novo hepatic lipogenesis in humans using stable isotopes.Journal of Clinical Investigation 87, 1841–1852.CrossRefGoogle ScholarPubMed
Issekutz, B, Bortz, WM, Miller, HI and Paul, P (1967) Turnover rate of plasma FFA in humans and in dogs.Metabolism 16, 1001–1009.CrossRefGoogle ScholarPubMed
Jones, PH, Lichtenstein, AH and Schaefer, EJ (1994) Interaction of dietary fat saturation and cholesterol level on cholesterol synthesis measured using deuterium incorporation.Journal of Lipid Research 35, 1093–1101.CrossRefGoogle ScholarPubMed
Jones, PH, Scanu, AM and Schoeller, DA (1988) Plasma cholesterol synthesis using deuterated water in humans: effect of short-term food restriction.Journal of Laboratory and Clinical Medicine 111, 627–633.Google Scholar
Jungas, RL (1968) Fatty acid synthesis in adipose tissue incubated in tritiated water.Biochemistry 7, 3708–3717.CrossRefGoogle ScholarPubMed
Kaluzny, MA, Duncan, LA, Merit, MV and Epps, DE (1985) Rapid separation of lipid classes in high yield and purity using bonded phase columns.Journal of Lipid Research 26, 135–140.Google Scholar
Leitch, CA and Jones, PJH (1991) Measurement of triglyceride synthesis in humans using deuterium oxide and isotope ratio mass spectrometry.Biological Mass Spectrometry 20, 392–396.Google Scholar
Leitch, CA and Jones, PJH (1993) Measurement of human lipogenesis using deuterium incorporation.Journal of Lipid Research 34, 157–163.CrossRefGoogle ScholarPubMed
Lifson, N and McLintock R (1966) Theory of the use of turnover rates of body water for measuring energy and material balance.Journal of Theoretical Biology 12, 46–74.CrossRefGoogle ScholarPubMed
Mackness, MI & Durrington, PN (1992) Lipoprotein separation and analysis for clinical studies. In Lipoprotein Analysis, pp. 1–42 [Converse, CA & Skinner, ER, editors]. Oxford: Oxford University Press.Google Scholar
Midwood, AJ, Haggarty, P and McGaw BA (1993) The doubly labeled water method; errors due to deuterium exchange and sequestration in ruminants.American Journal of Physiology 264, R561–R567.Google Scholar
Norum, KR, Berg, T, Helgerud, P and Drevon, CA (1983) Transport of cholesterol.Physiological Reviews 63, 1343–1419.Google Scholar
Ritenberg, D and Schoenheimer, R (1937) Deuterium as an indicator in the study of intermediary metabolism.Journal of Biological Chemistry 121, 235–253.Google Scholar
Sjostrom, L (1973) Carbohydrate stimulated fatty acid synthesis. de novo in human adipose tissue of different cellular types.Acta Medica Scandinavica 194, 387–404.CrossRefGoogle ScholarPubMed
Snyder, WS, Fish, BR, Bernard, SR, Ford, MR and Muir, JR (1968) Urinary excretion of tritium following exposure of man to HTO — a two exponential model.Physics in Medicine and Biology 13, 547–559.CrossRefGoogle Scholar
Spady, DK and Dietschy, JM (1983) Sterol synthesis in vivo in 18 tissues of the squirrel monkey, guinea pig, rabbit, hamster, and rat.Journal of Lipid Research 24, 303–315.CrossRefGoogle Scholar
Wong, WW, Hachey, DL, Insull, W, Opekun, AR and Klein, PD (1993) Effect of dietary cholesterol on cholesterol synthesis in breast-fed and formula-fed infants.Journal of Lipid Research 34, 1403–1411.CrossRefGoogle ScholarPubMed