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Functional characterization of three clones of the human intestinal Caco-2 cell line for dietary lipid processing

Published online by Cambridge University Press:  09 March 2007

Séverine Salvini ,
Monique Charbonnier ,
Catherine Defoort ,
Christian Alquier  and
Denis Lairon
Séverine Salvini
Affiliation:
Unité 476 INSERM, Nutrition humaine et lipides, 18 Avenue Mozart, 13009 Marseille, France
Monique Charbonnier
Affiliation:
Unité 476 INSERM, Nutrition humaine et lipides, 18 Avenue Mozart, 13009 Marseille, France
Catherine Defoort
Affiliation:
Unité 476 INSERM, Nutrition humaine et lipides, 18 Avenue Mozart, 13009 Marseille, France Laboratoire de Chimie Analytique, Faculté de Pharmacie, 27 Bd J Moulin, 13005 Marseille, France
Christian Alquier
Affiliation:
Unité 476 INSERM, Nutrition humaine et lipides, 18 Avenue Mozart, 13009 Marseille, France
Denis Lairon*
Affiliation:
Unité 476 INSERM, Nutrition humaine et lipides, 18 Avenue Mozart, 13009 Marseille, France
*
*Corresponding author: Dr Denis Lairon, fax +33 491 75 15 62, email lairon@marseille.inserm.fr
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Abstract

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We aimed to improve the use of the human intestinal Caco-2 cell line for studying dietary lipid and cholesterol processing by using isolated pure clones (). Three clones (TC7, PD7 and PF11) were grown as monolayers on semi-permeable filters and compared for cell viability, fatty acid and cholesterol apical uptake or basolateral secretion, apolipoprotein B-48 basolateral secretion and 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase activity. The TC7 clone showed the best viability upon apical incubation with mixed micelles and should be preferred for routine work. Short-term (3·0 h) rates of apical uptake of cholesterol were not different with the three clones, whereas the rate of apical uptake of oleic acid (18 : 1) was lower (P<0·05) with PF11 (250·6 nmol/mg) and the basolateral secretion of cholesterol and oleic acid was lower with the TC7 clone (0·40 and 29·1 nmol/mg respectively). The secretion of apolipoprotein B-48 basolaterally was about 2-fold lower than from PD7 clone. The basal levels of HMG-CoA reductase activity were significantly different (P<0·05; TC7>PF11>PD7). The down-regulation of the enzyme activity was moderate (range 13·8–21·0 %) and comparable in the presence of apical micellar cholesterol, but was much marked upon basolateral incubation with LDL (range 34·0–53·6 %), especially for the PD7 clone. In conclusion, the Caco-2 clones characterized here proved to be particularly suitable for studying lipid nutrients processing. Because these three clones exhibit some different metabolic capabilities, they provide a new tool to study intestinal response to lipid nutrients.

Keywords

CholesterolLipidsAbsorptionIntestine
Type
Research Article
Information
British Journal of Nutrition , Volume 87 , Issue 3 , March 2002 , pp. 211 - 217
Copyright
Copyright © The Nutrition Society 2002

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