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Guidelines for using in vitro methods to study the effects of phyto-oestrogens on bone

Published online by Cambridge University Press:  26 October 2011

Michèle Lieberherr ,
Giulia Cournot  and
Simon P. Robins
Michèle Lieberherr*
Affiliation:
Laboratoire de Nutrition et de Sécurité Alimentaire, Institut National de la Recherche Agronomique, F-78350 Jouy-en-Josas, France
Giulia Cournot
Affiliation:
Laboratoire de Nutrition et de Sécurité Alimentaire, Institut National de la Recherche Agronomique, F-78350 Jouy-en-Josas, France
Simon P. Robins
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, UK
*
*Corresponding author: Dr M. Lieberherr, fax +33 1 34 65 23 11, email lieberhe@jouy.inra.fr
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These guidelines review the relevant literature on the way plant phyto-oestrogens act on bone and the responsiveness of different bone cell systems to phyto-oestrogenic compounds. The primary emphasis is on the experimental conditions used, the markers available for assessing osteoblast and osteoclast function, and their expected sensitivity. Finally, we assess the published results to derive some general recommendations for in vitro experiments in this area of research.

Keywords

Phyto-oestrogensOsteoblastOsteoclastOsteoprogenitor bone marrow cells
Type
Research Article
Information
British Journal of Nutrition , Volume 89 , Issue S1: Effects of Phyto-oestrogens on Bone Health: the VENUS Concerted Action , June 2003 , pp. S59 - S73
Copyright
Copyright © The Nutrition Society 2003

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