Hostname: page-component-cd9895bd7-lnqnp Total loading time: 0 Render date: 2024-12-27T12:09:13.188Z Has data issue: false hasContentIssue false
  • English
  • Français

Haematological and blood chemistry changes in ewes and lambs following supplementation with vitamin E and selenium

Published online by Cambridge University Press:  09 March 2007

G. M. J. Horton ,
W. L. Jenkins  and
Rosemarie Rettenmaier
G. M. J. Horton
Affiliation:
Faculty of Veterinary Science, University of Pretoria, Onderstepoort, Republic of South Africa
W. L. Jenkins
Affiliation:
Faculty of Veterinary Science, University of Pretoria, Onderstepoort, Republic of South Africa
Rosemarie Rettenmaier
Affiliation:
F. Hoffmann-La Roche & Co., Basle, Switzerland
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. Four methods of vitamin E and selenium supplementation were evaluated using thirty-nine pregnant ewe-lambs fed on a ration containing 0.043 mg Se/kg and 25 mg vitamin E/kg. Treatments were control, fortified mineral mix (ESe salt) (300 mg vitamin E, 3 mg Se), ruminal Se pellets (505 mg Se), drench (300 mg vitamin E, 3 mg Se) and intramuscular injection (600 mg vitamin E, 3 mg Se). Only ewes were supplemented, commencing approximately 50 d before parturition.

2. Birth weights were similar for all treatments and live-weight gains of lambs to 56 d of age were improved in all supplemented groups (P < 0.05). There were no clinical cases of nutritional muscular dystrophy.

3. Se concentrations in whole blood were more than doubled in both lambs and ewes drenched or injected; responses to ESe salt and pellets were much smaller.

4. Plasma tocopherol levels were increased in injected dams and their lambs (P < 0.001).

5. Haemoglobin concentration and erythrocyte counts were significantly higher (P < 0.01) in control ewes and lambs than in treated lambs.

6. Lactate dehydrogenase (EC 1.1.1.27), creatine kinase (EC 2.7.3.2) and aspartate aminotransferase (EC 2.6.1.1) activities were increased in lambs from control, ESe salt and pellet groups (P < 0.001). Glutathione peroxidase (EC 1.1.1.1.9) activity responded to Se supplementation in both ewes and their lambs (P < 0.001) and the response was highest in the injected group, followed, in order, by the drench, pellet, ESe salt and control groups.

7. These studies indicated that in terms of the haematological and blood chemistry changes investigated, the intramuscular injection was most effective, followed by the oral drench. Ruminal pellets and fortified salt were less satisfactory.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 40 , Issue 2 , September 1978 , pp. 193 - 203
Copyright
Copyright © The Nutrition Society 1978

References

Alderson, N. E., Mitchell, G. E., Little, C. O., Warner, R. L. & Tucker, R. E. (1971). J. Nutr. 101, 655.CrossRefGoogle Scholar
Allaway, W. H. & Hodgson, J. F. (1964). J. Anim. Sci. 23, 271.CrossRefGoogle Scholar
Ammerman, C. B. & Miller, S. M. (1975). J. Dairy Sci. 58, 1561.CrossRefGoogle Scholar
Andrews, E. D., Grant, A. B. & Brunswick, L. F. C. (1974). N.Z. vet. J. 22, 46.CrossRefGoogle Scholar
Andrews, E. D., Hogan, K. G. & Sheppard, A. D. (1976). N.Z. vet. J. 24, 111.CrossRefGoogle Scholar
Association of Official Agricultural Chemists (1970). Official Methods of Analysis, 11th ed.Washington, DC: Association of Official Agricultural Chemists.Google Scholar
Boyd, J. W., Leyland, A. & Baker, J. R. (1975). Vet. Rec. 98, 490.CrossRefGoogle Scholar
Buchanan-Smith, J. G., Nelson, E. C., Osburn, B. I., Wells, M. E. & Tillman, A. D. (1969). J. Anim. Sci. 29, 808.CrossRefGoogle Scholar
Burton, V., Keeler, R. F., Swingle, K. F. & Young, S. (1962). Am. J. vet. Res. 23, 96.Google Scholar
Cousins, F. B. & Cairney, I. M. (1961). Aust. J. agric. Res. 12, 927.CrossRefGoogle Scholar
Culik, R., Bacigalupo, F. A., Thorp, F., Luecke, R. W. & Nelson, R. H. (1951). J. Anim. Sci. 10, 1006.CrossRefGoogle Scholar
Godwin, K. O., Fuss, C. N. & Kuchel, R. E. (1975). Aust. J. biol. Sci. 28, 251.CrossRefGoogle Scholar
Günzler, W. A., Kremers, H. & Flohe, L. (1974). Z. Klin. Chem. Klin. Biochem. 12, 444.Google Scholar
Hashim, S. A. & Schuttringer, G. R. (1966). Am. J. clin. Nutr. 19, 137.CrossRefGoogle Scholar
Hidiroglou, M., Jenkins, K. J. & Corner, A. H. (1972). Can. J. Anim. Sci. 52, 511.CrossRefGoogle Scholar
Hopkins, K. L., Pope, A. L., Baumann, C. A. & Kowalczyk, T. (1966). Wld Rev. Anim. Prod. 2, 101.Google Scholar
Horton, G. J. M., Owen, N. C., Horak, I. G. & Schröder, J. (1977). Jl S. Afr. vet. med. Ass. 48, 99.Google Scholar
International Union of Biochemistry (1965). Enzyme Nomenclature, p. 7. Amsterdam: Elsevier.Google Scholar
Karmen, A. (1955). J. clin. Invest. 34, 131.CrossRefGoogle Scholar
Lee, H. J. & Kuchel, R. E. (1969). In Research reports. Division of Nutritional Biochemistry, Adelaide, Australia: CSIRO.Google Scholar
Lindberg, P. & Lannek, N. (1970). In Trace Element Metabolism in Animals [Mills, C. F., editor]. Edinburgh: Livingstone.Google Scholar
Oksanen, H. E. (1967). In Selenium in Biomedicine [Muth, O. H., editor]. Westport, Connecticut: Avi Publishing Co.Google Scholar
Oldfield, J. E., Schubert, J. R. & Muth, O. H. (1963). J. agric. Fd Chem. 11, 388.CrossRefGoogle Scholar
Olson, O. E., Palmer, I. S. & Cary, E. E. (1975). J. Ass. off Analyt. Chem. 58, 117.Google Scholar
Paulson, G. D., Broderick, G. A., Baumann, C. A. & Pope, A. L. (1968). J. Anim. Sci. 27, 195.CrossRefGoogle Scholar
Perry, T. W., Beeson, W. M., Smith, W. H. & Mohler, M. T. (1976). J. Anim. Sci. 42, 192.CrossRefGoogle Scholar
Snedecor, G. W. & Cochran, W. G. (1967). Statistical Methods, 6th ed.Ames, Iowa: Iowa State University Press.Google Scholar
Tollersrud, S. & Ribe, O. (1967). Acta. vet. scand. 8, 1.CrossRefGoogle Scholar
Tucker, E. M. (1974). Res. vet. Sci. 16, 19.CrossRefGoogle Scholar
Weismann, U., Colombo, J. P., Adam, A. & Richterich, R. (1966). Enzym. Biol. Clin. 7, 266.CrossRefGoogle Scholar
Wroblewski, R. & LaDue, J. S. (1955). Proc. Soc. exp. Biol. Med. 90, 210.CrossRefGoogle Scholar