Hostname: page-component-cd9895bd7-jn8rn Total loading time: 0 Render date: 2024-12-27T11:20:22.898Z Has data issue: false hasContentIssue false
  • English
  • Français

High-fat diets and the immune response of C57 BI mice

Published online by Cambridge University Press:  09 March 2007

R. W. R. Crevel ,
J. V. Friend ,
B. F. J. Goodwin  and
W. E. Parish
R. W. R. Crevel
Affiliation:
Environmental Safety Laboratory, Unilever Research & Engineering, Colworth House, Sharnbrook, Bedford MK44 1LQ
J. V. Friend
Affiliation:
Environmental Safety Laboratory, Unilever Research & Engineering, Colworth House, Sharnbrook, Bedford MK44 1LQ
B. F. J. Goodwin
Affiliation:
Environmental Safety Laboratory, Unilever Research & Engineering, Colworth House, Sharnbrook, Bedford MK44 1LQ
W. E. Parish
Affiliation:
Environmental Safety Laboratory, Unilever Research & Engineering, Colworth House, Sharnbrook, Bedford MK44 1LQ
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

As a basis for studies of the influence of lipids on the immune response and health, adult C57 BI mice were fed for 10 weeks or longer on one of the following diets: high (200 g/kg) polyunsaturated fatty acid, high (200 g/kg) saturated fatty acid and low (50 g/kg) polyunsaturated fatty acid purified diets and a standard commercial diet. The three test-fat diets were compounded to have approximately the same energy content and the mice of each group maintained similar body-weights. High-fat diets significantly reduced their subsequent delayed hypersensitivity response to challenge after sensitization with tuberculin. Immunoglobulin (Ig)M antibody formation against Escherichia coli lipopolysaccaride was transiently decreased, but IgG antibody against sheep erythrocytes and killed salmonella vaccine, IgG and IgE antibodies against ovalbumin remained unaffected. Total and differential blood counts revealed no differences between mice on high-fat and control diets in either the absolute numbers or the proportions of the types of leukocytes. Studies on peritoneal macrophages from mice of each group showed no difference in morphology and they ingested non-toxic and toxic particles releasing similar amounts of lactate dehydrogenase (EC 1.1.1.27) and β-glucuronidase (EC 3.2.1.31) for each substance, indicating that there were no differences in viability or phagocytic function. The present study shows that the C57 BI mouse can provide a model for the investigation of some consequence of the reduced immunocompetence induced by high-fat diets.

Keywords

High fat dietImmune responseC57 Bl mice
Type
Diet and the Immune Response
Information
British Journal of Nutrition , Volume 67 , Issue 1 , January 1992 , pp. 17 - 26
Copyright
Copyright © The Nutrition Society 1992

References

Association of Official Agricultural Chemists (1975). Official Methods of Analysis. 12th ed. Washington, DC: ADAC.Google Scholar
Curtiss, L. K. & Edgington, T. S. (1979). Differential sensitivity of lymphocyte sub-populations to suppression by low density lipoprotein inhibitor, an immunoregulatory human serum low density lipoprotein. Journal of Clinical Investigation 63, 193–201.CrossRefGoogle Scholar
De Deckere, E. M. A., Verplanke, J., Blonk, C. G. & Van Nielen, W. G. L. (1988). Effects of type and amount of dietary fat on rabbit and rat lymphocyte proliferation in vitro. Journal of Nutrition 188, 11–18.CrossRefGoogle Scholar
DeWille, J. W., Fraker, P. J. & Romsos, D. R. (1979). Effects of essential fatty acid deficiency and various levels of polyunsaturated fatty acids on humoral immunity in mice. Journal of Nutrition 109, 1018–1027.CrossRefGoogle ScholarPubMed
DeWille, J. W., Fraker, P. J. & Romsos, D. R. (1981). Effects of dietary fatty acids on delayed type hypersensitivity in mice. Journal of Nutrition 111, 2039–2043.CrossRefGoogle ScholarPubMed
Dyer, C. A., Takagi, Y. & Curtiss, L. K. (1987). Macrophage-derived apolipoprotein E inhibits lymphocyte proliferation. Journal of Leukocyte Biology 42, 352 Abstr.Google Scholar
Eagle, H. (1953). Amino acid metabolism in mammalian cell cultures. Science 130, 432–437.CrossRefGoogle Scholar
Erickson, K. L. & Adams, D. A. (1983). Dietary modulation of B-cell responses. Federation Proceedings 42, 1187.Google Scholar
Erickson, K. L., McNeill, C. J., Gerschwin, M. E. & Ossman, J. B. (1980). Influence of dietary fat concentration and saturation on immune ontogeny in mice. Journal of Nutrition 110, 1555–1572.CrossRefGoogle ScholarPubMed
Friend, J. V., Lock, S. O., Gurr, M. I. & Parish, W. E. (1980). Effect of different dietary lipids on the immune response of Hartley strain guinea-pigs. International Archives of Allergy and Applied Immunology 62, 292–301.CrossRefGoogle ScholarPubMed
Goodwin, J. S. & Webb, D. R. (1980). Regulation of the immune response by prostaglandins. Clinical Immunology and Immunopathology 15, 106–122.CrossRefGoogle ScholarPubMed
Gordon, S., Werb, Z. & Cohn, Z. A. (1979). In In vitro Methods in Cell-mediated Immunity [Bloom, D., editor]. London: Academic Press.Google Scholar
Gurr, M. I., Robinson, M. P., Kirtland, K. & Widdowson, E. M. (1976). Body lipids of guinea-pigs exposed to different dietary fats from mid-gestation to 3 months of age. III. Nutrition and Metabolism 20, 364–377.CrossRefGoogle ScholarPubMed
Kollmorgen, G. M., Sansing, W. A., Lehman, A. A., Fisher, G., Longley, R. E., Alexander, S. S., King, M. M. & McCay, P. B. (1979). Inhibition of lymphocyte function in rats fed high fat diets. Cancer Research 39, 3458–3462.Google Scholar
McCoy, H., Williams, L. & Carroll, C. (1979). Interaction of dietary fat and route of immunisation in the immune response of the rat. Nutrition Reports International 19, 289–298.Google Scholar
Medawar, P., Hunt, R. C. & Martin, J. (1979). An influence of diet on transplantation immunity. Proceedings of the Royal Society B206, 265–280.Google Scholar
Mota, I., Wong, D., Sadun, E. H. & Gore, R. W. (1969). Mouse homocytotropic antibodies. In Cellular and Humoral Mechanisms in Anaphylaxis and Allergy pp. 23–36 [Movat, H. Z., editor]. Basel: Karger.Google Scholar
National Academy of Sciences (1978). Nutrient Requirements of Laboratory Animals 3rd ed. Washington, DC: National Academy of Sciences.Google Scholar
Ossman, J. B., Erickson, K. L. & Canolty, N. L. (1980). Effects of saturation and concentration of dietary fats on lymphocyte transformation in mice. Nutrition Reports International 22, 279–284.Google Scholar
Ovary, L., Caizza, S. A. & Kojima, S. (1975). PCA reactions with mouse antibodies in mice and rats. International Archives of Allergy and Applied Immunology 48, 16–21.CrossRefGoogle ScholarPubMed
Page, R. C., Davies, P. & Allison, A. C. (1973). Effects of dental plaque on the production and release of lysosomal enzymes by macrophages in culture. Archives of Oral Biology 18, 1481–1495.CrossRefGoogle ScholarPubMed
Pelus, L. M. & Strausser, H. R. (1977). Prostaglandins and the immune response. Life Sciences 20, 903–914.CrossRefGoogle ScholarPubMed
Pepe, M. G. & Curtiss, L. K. (1986). Apolipoprotein E is a biologically active constituent of the normal immunoregulatory lipoprotein, LDL-In. Journal of Immunology 136, 3716–3723.CrossRefGoogle ScholarPubMed
Santiago-Delpin, E. A. & Roman-Franco, A. A. (1980). Lymphocyte reactivity is diminished by lipid diets. Federation Proceedings 38, 1162.Google Scholar
Stenson, W. F. & Parker, C. W. (1980). Prostaglandins macrophages and immunity. Journal of Immunology 125, 1–5.CrossRefGoogle ScholarPubMed
Voller, A., Bidwell, D. & Bartlett, A. (1976). Microplate enzyme immunoassays for the immunodiagnosis of virus infections. In Manual of Clinical Immunology p. 506 [Rose, N. R. and Friedman, H., editors]. Washington: American Society of Microbiology.Google Scholar
Widdowson, E. M., Dauncey, M. J., Gairdner, D. M. T., Jonxis, J. H. P. & Pelikan-Filipkova, M. (1975). Body fat of British and Dutch infants. British Medical Journal i, 653–655.CrossRefGoogle Scholar
Wroblewski, F. & Ladau, J. S. (1955). Lactic dehydrogenase activity in blood. Proceedings of the Society of Experimental Biology and Medicine 90, 210–215.CrossRefGoogle ScholarPubMed