Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-27T09:01:49.248Z Has data issue: false hasContentIssue false
  • English
  • Français

Improvement of the probiotic effect of micro-organisms by their combination with maltodextrins, fructo-oligosaccharides and polyunsaturated fatty acids

Published online by Cambridge University Press:  09 March 2007

A. Bomba ,
R. Nemcová ,
S. Gancarcíková ,
R. Herich ,
P. Guba  and
D. Mudronová
A. Bomba*
Affiliation:
Research Institute of Veterinary Medicine, Hlinkova 1/A, 040 01, Slovak Republic
R. Nemcová
Affiliation:
Research Institute of Veterinary Medicine, Hlinkova 1/A, 040 01, Slovak Republic
S. Gancarcíková
Affiliation:
Research Institute of Veterinary Medicine, Hlinkova 1/A, 040 01, Slovak Republic
R. Herich
Affiliation:
University of Veterinary Medicine, Komenského 73 040 01, Košice, Slovak Republic
P. Guba
Affiliation:
Research Institute of Veterinary Medicine, Hlinkova 1/A, 040 01, Slovak Republic
D. Mudronová
Affiliation:
Research Institute of Veterinary Medicine, Hlinkova 1/A, 040 01, Slovak Republic
*
*Corresponding author: Dr A. Bomba, fax +421 55 63 318 53, email bomba@vuvm.sk
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Probiotics could represent an effective alternative to the use of synthetic substances in nutrition and medicine. The data concerning the efficacy of probiotics are often contradictory. This paper focuses on the enhancement of the efficacy of probiotics by their combination with synergistically acting components of natural origin. Maltodextrins can be obtained by enzymatic hydrolysis of starch and are suitable for consumption. Administration of Lactobacillus paracasei together with maltodextrin decreased the number of Escherichia coli colonising the jejunal mucosa of gnotobiotic piglets by 1 logarithm compared to the control group. Fructo-oligosaccharides (FOS) are naturally occurring oligosaccharides, mainly of plant origin. L. paracasei administered in combination with FOS significantly increased counts of Lactobacillus spp., Bifidobacterium spp., total anaerobes and total aerobes compared to the control group as well as the L. paracasei group. It also significantly decreased Clostridium and Enterobacterium counts in the faeces of the weanling piglets compared with the control group. Dietary lipids influence the gastrointestinal microbiota and specifically the population of lactic acid bacteria. In gnotobiotic piglets the oral administration of an oil containing polyunsaturated fatty acids (PUFA) significantly increased the number of L. paracasei adhering to jejunal mucosa compared to the control group. Our results showed that maltodextrin KMS X-70 and PUFA can be used to enhance the effect of probiotic micro-organisms in the small intestine, and similarly FOS enhance the effect of probiotic micro-organisms in the large intestine.

Keywords

ProbioticsMaltodextrinFructo-oligosaccharidesPolyunsaturated fatty acids
Type
Research Article
Information
British Journal of Nutrition , Volume 88 , Issue S1 , September 2002 , pp. S95 - S99
Copyright
Copyright © The Nutrition Society 2002

References

Baird, DM (1977) Probiotics help boost feed efficiency. Feedstuffs 49, 11.Google Scholar
Bekaert, H, Moermans, R & Eeckhout, W (1996) Influence d'une culture de levure vivante (Levucell SB2) dans un aliment pour porcelets sevrés sur les performances zootechniques et sur la fréquence des diarrhées (Influence of the live culture of yeasts in feed on the performance and incidence of diarrhea in piglets). Annales de Zootechnie 45, 369–376.CrossRefGoogle Scholar
Bomba, A, Kaštel', R, Gancarčíková, S, Nemcová, R, Herich, R & Čížek, M (1996) The effect of Lactobacilli inoculation on organic acid levels in the mucosal film and the small intestine contents in gnotobiotic pigs. Berliner und Münchener Tierärztliche Wochenschrift 109, 428–430.Google ScholarPubMed
Bomba, A, Gancarčíková, S, Nemcová, R, Herich, R, Kaštel', R, Depta, A, Demeterová, M, Ledecký, V & Žitňan, R (1998) The effect of lactic acid bacteria on intestinal metabolism and metabolic profile of gnotobiotic pigs. Deutsche Tierärztliche Wochenschrift 105, 384–389.Google Scholar
Burgstaller, G, Ferstl, R & Apls, H (1984) Zum Zusatz von Milchsäurebakterien (Streptococcus faecium SF-68) im Milchaustauschfuttermittel fur Mastkälber (The addition of lactic acid bacteria to a milk replacer for calf feeding). Zuchtungskunde 56, 156–162.Google Scholar
Chateau, N, Castellanos, I & Deschamps, AM (1993) Distribution of pathogen inhibition in the Lactobacillus isolates of a commercial probiotic consortium. Journal of Applied Bacteriology 74, 36–40.CrossRefGoogle ScholarPubMed
Chesson, A (1993) Probiotics and other intestinal mediators. In Principles of Pig Science, pp. 197–214 [Cole, DJA, Wisiman, J-MA and Varley, MA, editors]. Loughborough: Nottingham University Press.Google Scholar
De Cupere, F, Deprez, P, Demeulenaere, D & Muylle, E (1992) Evaluation of the effect of 3 probiotics on experimental Escherichia coli enterotoxaemia in weaned piglets. Journal of Veterinary Medicine B 39, 277–284.CrossRefGoogle ScholarPubMed
Depta, A, Rychlik, R, Nieradka, R, Rotkiewicz, T, Kujawa, K, Bomba, A & Grabowska-Świecicka, G (1998) The influence of alimentary tract colonization with Lactobacillus sp. strains on chosen metabolic profile indices in piglets. Polish Journal of Veterinary Science 1–2., 3–7.Google Scholar
Freter, R (1992) Factors affecting the microecology of the gut. In Probiotics: The Scientific Basis, pp. 111–114 [Fuller, R, editor]. London: Chapman and Hall.CrossRefGoogle Scholar
Fuller, R (1992) The effect of probiotics on the gut microbiology of farm animals. In The Lactic Acid Bacteria, pp. 171–192 [Wood, BJB, editor]. London: Elsevier Applied Science.Google Scholar
Gálfi, P & Bokori, J (1990) Feeding trial in pigs with a diet containing sodium n-butyrate. Acta Veterinaria Hungarica 38, 3–17.Google Scholar
Gibson, GR & Roberfroid, MB (1995) Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. Journal of Nutrition 125, 1401–1412.CrossRefGoogle ScholarPubMed
Goldin, BR & Gorbach, SL (1977) Alterations in fecal microflora enzymes related to diet, age, lactobacillus supplements and dimethylhydrazine. Cancer 40, 2421–2426.3.0.CO;2-I>CrossRefGoogle ScholarPubMed
Goldin, BR & Gorbach, SL (1984) The effect of milk and lactobacillus feeding on human intestinal bacterial enzyme activity. American Journal of Clinical Nutrition 39, 756–761.CrossRefGoogle ScholarPubMed
Hale, OM & Newton, GL (1979) Effects of a non-viable lactobacillus species fermentation product on performance of pigs. Journal of Animal Science 48, 770–775.Google Scholar
Kankaanpää, PE, Salminen, SJ, Isolauri, E & Lee, YK (2001) The influence of polyunsaturated fatty acids on probiotic growth and adhesion. FEMS Microbiology Letters 194, 149–153.CrossRefGoogle Scholar
Kimura, N, Yoshikane, M, Kobayashi, A & Mitsuoka, T (1983) An application of dried bifidobacteria preparation to scouring animals. Bifidobacteria and Microflora 2, 41–55.CrossRefGoogle Scholar
Kontula, P, Jaskari, J, Nollet, L, De Smet, I, von Wright, A, Poutanen, K & Mattila-Sandholm, T (1998) The colonization of a stimulator of the human intestinal microbial ecosystem by a probiotic strain fed on a fermented oat bran product: effects on the gastrointestinal microbiota. Applied Microbiology and Biotechnology 50, 246–252.Google Scholar
Maeng, WJ, Kim, CW & Shin, HT (1989) Effect of feeding lactic acid bacteria concentrate (LBC, Streptococcus faecium Cernelle 68) on the growth rate and prevention of scouring in piglets. Korean Journal of Animal Science 31, 318.Google Scholar
Mann, GV (1977) A factor of yoghurt which lowers cholesterolaemia in man. Atherosclerosis 26, 335–340.CrossRefGoogle Scholar
Nousiainen, J & Setälä, J (1993) Lactic acid bacteria as animal probiotics. In Lactic Acid Bacteria, pp. 315–356 [Salminen, S and von Wright, A, editors]. New York: Marcel Dekker.Google Scholar
Oku, T, Tokunaga, T & Hosoga, N (1984) Nondigestibility of a new sweetener Neosugar in the rat. Journal of Nutrition 114, 1574–1581.CrossRefGoogle ScholarPubMed
Perdigón, G & Alvarez, S (1992) Bacterial interactions in the gut. In Probiotics. The Scientific Basis, pp. 145–180 [Fuller, R, editor]. London: Chapman and Hall.CrossRefGoogle Scholar
Piard, JC & Desmazeaud, M (1991) Inhibiting factors produced by lactic acid bacteria. 1. Oxygen metabolites and catabolism and-products. Lait 71, 525–541.Google Scholar
Pollmann, DS, Danielson, DM, Wren, WB, Peo, ER & Shahani, KM (1980) Influence of Lactobacillus acidophillus inoculum on gnotobiotic and conventional pigs. Journal of Animal Science 51, 629–637.CrossRefGoogle Scholar
Reddy, GV, Shahani, KM & Banerjee, MR (1973) Inhibitory effect of the yogurt on Ehrlich ascites tumor cell proliferation. Journal of National Cancer Institute 50, 815–817.CrossRefGoogle ScholarPubMed
Ringo, E, Bendiksen, HR, Gausen, SJ, Sundsfjord, A & Olsen, RE (1998) The effect of dietary fatty acids on lactic acid bacteria associated with the epithelial mucosa and from faecalia of Arctic charr, Salvelinus alpinus (L.). Journal of Applied Microbiology 85, 855–864.CrossRefGoogle ScholarPubMed
Roberfroid, MB (1998) Prebiotics and synbiotics: concepts and nutritional properties. British Journal of Nutrition 80, Suppl. 2, S197–S202.CrossRefGoogle ScholarPubMed
Rowland, IR & Grasso, P (1975) Degradation of N-nitrosamines by intestinal bacteria. Applied Microbiology 29, 7–12.Google Scholar
Stavric, S, Gleeson, TM, Blanchfield, B & Pivnick, H (1987) Role of adhering microflora in competitive exclusion of Salmonella from young chicks. Journal of Food Protection 50, 928–932.Google Scholar
Stavric, S & Kornegay, ET (1995) Microbial probiotic for pigs and poultry. In Biotechnology in Animal Feeds and Animal Feeding, pp. 205–231 [Wallace, RJ and Chesson, A, editors]. Weinheim: VCH Verlagsgesellschaft mbH.CrossRefGoogle Scholar
Vandenbergh, PA (1993) Lactic acid bacteria, their metabolic products and interference with microbial growth. FEMS Microbiology Review 12, 221–238.Google Scholar
Yadava, JNS, Gupta, S, Ahmad, I, Varma, N & Tandon, JS (1995) Neutralization of enterotoxins of E coli by coleonol (forskolin) in rabbit and guinea pig ileal loop models. Indian Journal of Animal Science 65, 1177–1181.Google Scholar
Zacconi, C, Bottazzi, V, Rebecchi, A, Bosi, E, Sarra, PG & Tagliaferi, L (1992) Serum cholesterol levels in axenic mice colonized with Enterococcus faecium and Lactobacillus acidophilus. Microbiologica 15, 413–418.Google ScholarPubMed