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In vivo total body water assessment by total body electrical conductivity in rats suffering perturbations of water compartment equilibrium

Published online by Cambridge University Press:  09 March 2007

N. Battistini ,
F. Virgili ,
G. Bedogni ,
G. R. Gambella  and
A. Bini
N. Battistini
Affiliation:
Institute of Human Physiology and Pathology, University of Modena, via Campi 287, 41100 Modena, Italy
F. Virgili
Affiliation:
National Institute of Nutrition, via Ardeaiina 546, 00178 Rome, Italy
G. Bedogni
Affiliation:
Institute of Human Physiology and Pathology, University of Modena, via Campi 287, 41100 Modena, Italy
G. R. Gambella
Affiliation:
Institute of Pathology, University of Genova, via L. B. Alberti 2, 16132, Italy
A. Bini
Affiliation:
Institute of Human Physiology and Pathology, University of Modena, via Campi 287, 41100 Modena, Italy
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Abstract

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Total body electrical conductivity (TOBEC) is a simple and non-invasive method for the assessment of body composition in vivo. Information regarding the applicability of TOBEC in the condition of abnormal fluid balance is scarce. In the present paper we give the results of the comparison between TOBEC and total body water (TBW; assessed by the tritium dilution technique) in three groups of animals: (1) healthy (n 17), (2) expanded fluid volume by secondary biliary cirrhosis (SBC; n 9) and (3) Fiirosemide®-treated rats (n 9). The TOBEC score and TBW by tritium dilution were found to be highly correlated in the pooled sample (r 0·90) and in normal (r 0.·87), SBC (r 0·73) and Furosemide-treated (r 0·89) rats. However, the relationship between TOBEC and TBW, described by least-squares regression analysis, was found to be similar for SBC and normal rats but was significantly different for Furosemide-treated and normal rats. These findings suggest that TOBEC is unable to track TBW accurately when the ratio between intracellular and extracellular water is chronically or acutely altered.

Keywords

Total body waterTritium dilution spaceTotal body electrical conductivity.
Type
Body Composition Measurements in Vivo
Information
British Journal of Nutrition , Volume 70 , Issue 2 , September 1993 , pp. 433 - 438
Copyright
Copyright © The Nutrition Society 1993

References

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