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The influence of different amounts of n-3 polyunsaturated fatty acids on bleeding time and in vivo vascular reactivity

Published online by Cambridge University Press:  09 March 2007

Gwenda Mark  and
T. A. B. Sanders
Gwenda Mark
Affiliation:
Department of Pharmacology, King's College, University of London, Campden Hill Road, LondonW8 7AH
T. A. B. Sanders
Affiliation:
Department of Nutrition and Dietetics, King's College, University of London, Campden Hill Road, LondonW8 7AH
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Abstract

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Mesenteric bleeding time, mesenteric vascular reactivity, platelet and erythrocyte lipid fatty acid composition were measured at 2–3 weeks, 5–6 weeks and 11–22 weeks in normotensive Wistar rats, fed on high (6·5% energy) or moderate (1·6% energy) intakes of eicosapentaenoic acid (20: 5n−3; EPA) as fish oil, compared with controls fed on a diet devoid of EPA. All diets contained the same level of linoleic acid (4% energy): the moderate- and high-EPA diets also contained 1·1 and 4·4% of the energy as docosahexaenoic acid (22:6n−3) respectively. Moderate, but not high, intakes of EPA increased mesenteric bleeding time. Similar reductions in erythrocyte and platelet arachidonic acid (20: 4n−6) occurred in animals fed on either high or low amounts of EPA, but the proportion of EPA increased dose-dependently. At high intakes of EPA the proportion of oleic acid in platelets and erythrocytes was decreased. Blood pressure platelet counts, mesenteric vessel diameter and mesenteric vascular reactivity to vasopressin were unaffected by treatment. High intakes of fish oil led to a slight fall in packed cell volume. In a second experiment bleeding time and mesenteric vascular reactivity to noradrenaline were increased 2–4 weeks after receiving a moderate intake of EPA and these effects persisted 5–21 d after switching to a control diet. A similar increase in vascular reactivity to noradrenaline was observed in animals given indomethacin (6 mg/kg) but not in those given aspirin (20 mg/kg).

Keywords

HaemostasisEicosapentaenoic acidDocosahexaenoic acidNoradrenaline
Type
Amino acid digestion in the pig and adult human
Information
British Journal of Nutrition , Volume 71 , Issue 1 , January 1994 , pp. 43 - 52
Copyright
Copyright © The Nutrition Society 1994

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