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Insulin-like growth factor-I, but not growth hormone, is dependent on a high protein intake to increase nitrogen balance in the rat

Published online by Cambridge University Press:  09 March 2007

Myriam Sanchez-Gomez ,
Kjell Malmlöf ,
Wilson Mejia ,
Antonio Bermudez ,
Maria Teresa Ochoa ,
Stella Carrasco-Rodriguez  and
Anna Skottner
Myriam Sanchez-Gomez
Affiliation:
Department de Chemistry, Universidad Nacional de Colombia, Santafé de Bogotá, Colombia
Kjell Malmlöf*
Affiliation:
Department of Pharmacology, Peptide Hormones, Pharmacia, Stockholm, Sweden
Wilson Mejia
Affiliation:
Department de Chemistry, Universidad Nacional de Colombia, Santafé de Bogotá, Colombia
Antonio Bermudez
Affiliation:
Department de Chemistry, Universidad Nacional de Colombia, Santafé de Bogotá, Colombia
Maria Teresa Ochoa
Affiliation:
Department de Chemistry, Universidad Nacional de Colombia, Santafé de Bogotá, Colombia
Stella Carrasco-Rodriguez
Affiliation:
Department de Chemistry, Universidad Nacional de Colombia, Santafé de Bogotá, Colombia
Anna Skottner
Affiliation:
Department of Pharmacology, Peptide Hormones, Pharmacia, Stockholm, Sweden
*
*Corresponding author: Dr Kjell Malmlöf, present address Novo Nordisk A/S, GH Biology, Niels Steensens Vej 8, 2820 Gentofte, Denmark, fax +45 44 43 80 06, email Kmal@novo.dk
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Abstract

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The aim of the present study was to investigate the influence of dietary protein level on the protein anabolic effects of growth hormone (GH) and insulin-like growth factor-I (IGF-I). Female growing rats were fed on either a high- or a low-protein diet with crude protein contents of 222 and 83 g/kg respectively. The diets contained the same amount of metabolizable energy (15·1 MJ/kg) and were given during a 14 d period. During the same time, three groups of rats (n 8) on each diet received subcutaneous infusions of either saline, recombinant human GH (rhGH) or recombinant human IGF-I (rhIGF-I). rhGH and rhIGF-I were given in doses of 360 and 500 μg/d respectively. The low-protein diet alone reduced significantly (P < 0·05) IGF-I concentrations in serum and in tissue taken from the gastrocnemius muscle as well as IGF-I mRNA from the same muscle. The responses to rhGH and rhIGF-I in terms of muscle IGF-I and its mRNA were variable. However, when rhIGF-I was infused into rats on the high-protein diet, significantly elevated levels of IGF-I in muscle tissues could be observed. This was associated with a significantly (P < 0·05) increased N balance, whereas rhGH significantly (P < 0·05) enhanced the N balance in rats on the low-protein diet. Thus, it can be concluded that the level of dietary protein ingested regulates not only the effect of IGF-I on whole-body N economy but also the regulation of IGF-I gene expression in muscles. The exact mechanism by which GH exerts its protein anabolic effect, however, remains to be elucidated.

Keywords

Dietary proteinGrowth hormoneInsulin-like growth factorNitrogen balance
Type
Research Article
Information
British Journal of Nutrition , Volume 81 , Issue 2 , February 1999 , pp. 145 - 152
Copyright
Copyright © The Nutrition Society 1999

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