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Intestinal metabolism of rye lignans in pigs

Published online by Cambridge University Press:  09 March 2007

L. V. Glitsø ,
W. M. Mazur ,
H. Adlercreutz ,
K. Wähälä ,
T. Mäkelä ,
B. Sandström  and
K. E. Bach Knudsen
L. V. Glitsø*
Affiliation:
Danish Institute of Agricultural Sciences, Dept. of Animal Nutrition and Physiology, P.O. Box 50, Research Centre Foulum, DK-8830, Tjele, Denmark Research Dept. of Human Nutrition, The Royal Veterinary and Agricultural University, DK-1958 Frederiksberg C, Denmark
W. M. Mazur
Affiliation:
Dept. of Clinical Chemistry University of Helsinki and Folkhälsan Research Center, P.O. Box 60, FIN-00014, University of Helsinki, Finland
H. Adlercreutz
Affiliation:
Dept. of Clinical Chemistry University of Helsinki and Folkhälsan Research Center, P.O. Box 60, FIN-00014, University of Helsinki, Finland
K. Wähälä
Affiliation:
Dept. of Chemistry, P.O. Box 55, 00014 University of Helsinki, Finland
T. Mäkelä
Affiliation:
Dept. of Chemistry, P.O. Box 55, 00014 University of Helsinki, Finland
B. Sandström
Affiliation:
Research Dept. of Human Nutrition, The Royal Veterinary and Agricultural University, DK-1958 Frederiksberg C, Denmark
K. E. Bach Knudsen
Affiliation:
Danish Institute of Agricultural Sciences, Dept. of Animal Nutrition and Physiology, P.O. Box 50, Research Centre Foulum, DK-8830, Tjele, Denmark
*
*Corresponding author: Dr Vibe Glitsø, fax +45 8999 1378, email vibe.glitsoe@arlafoods.com
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Abstract

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To study the intestinal metabolism of lignans, the concentrations of plant and mammalian lignans in intestinal digesta sampled along the intestinal tract of pigs were determined by isotope dilution GC–MS. The pigs were fed rye-bread diets made from either whole rye-grains or rye-grain milling fractions enriched in pericarp–testa, aleurone or endosperm cells. The content and characteristics of dietary fibre varied between diets and had been shown to induce different colon fermentation patterns. As the metabolism of lignans depends on the action of the intestinal flora, we tested whether the rye-bread diets influence the metabolism of lignans. In the ileum, the lignans were mainly present as conjugated plant lignans, which were determined only when the analytical procedure included a hydrolysis step. High recovery of dietary lignans in the ileum may indicate that the lignans enter the enterohepatic circulation. In addition, two to three times the intake of lignans were recovered in the faeces when the diets had a high content of dietary fibre suggesting underestimation of plant lignans in the diet. Most of the plant lignans disappeared from the intestinal tract between the terminal ileum and the caecum. The intestinal concentrations and the disappearance of lignans correlated with the content of lignans in the diet, being highest on the pericarp–testa diet and lowest on the endosperm diet. No effect of fermentation pattern on the intestinal metabolism of lignans was observed. The lignans were liberated from the pericarp–testa diet although the plant cell walls remained largely undegraded.

Keywords

LignansColon fermentationDietary fibre
Type
Research Article
Information
British Journal of Nutrition , Volume 84 , Issue 4 , October 2000 , pp. 429 - 437
Copyright
Copyright © The Nutrition Society 2000

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