Hostname: page-component-78c5997874-j824f Total loading time: 0 Render date: 2024-11-10T14:07:20.978Z Has data issue: false hasContentIssue false
  • English
  • Français

L-Arginine attenuates xanthine oxidase and myeloperoxidase activities in hearts of rats during exhaustive exercise

Published online by Cambridge University Press:  08 March 2007

Wan-Teng Lin ,
Suh-Ching Yang ,
Shiow-Chwen Tsai ,
Chi-Chang Huang  and
Ning-Yuean Lee
Wan-Teng Lin
Affiliation:
Fu-Jen Catholic University, Department of Nutrition and Food Sciences, TaipeiTaiwan, Republic of China De Lin Institute of Technology, Taipei, Taiwan, Republic of China
Suh-Ching Yang
Affiliation:
School of Nutrition and Health Sciences, Taipei Medical University, Taipei, Taiwan, Republic of China
Shiow-Chwen Tsai
Affiliation:
Shin Kong Wu Ho-Su Memorial Hospital, Central Laboratory, Taipei, Taiwan, Republic of China
Chi-Chang Huang
Affiliation:
School of Nutrition and Health Sciences, Taipei Medical University, Taipei, Taiwan, Republic of China
Ning-Yuean Lee*
Affiliation:
Fu-Jen Catholic University, Department of Nutrition and Food Sciences, TaipeiTaiwan, Republic of China
*
*Corresponding author: Dr Ning-Yuean Lee, fax +886 2 2904 3234, email als2046@mails.fju.edu.tw
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The present study was to investigate the effects of l-arginine (l-Arg) supplementation on cardiac oxidative stress and the inflammatory response in rats following acute exhaustive exercise on a treadmill. Rats were randomly divided into four groups: sedentary control (SC); SC with l-Arg treatment (SC+Arg); exhaustive exercise (E); exhaustive exercise with l-Arg treatment (E+Arg). Rats in groups SC+Arg and E+Arg received a 2% l-Arg diet. Rats in groups E and E+Arg performed an exhaustive running test on a treadmill at a final speed of 30m/min, 10% grade, at approximately 70–75% VO2max. The results showed a significant increase in cardiac xanthine oxidase (XO) and myeloperoxidase activities and membrane lipid peroxidation endproduct (malondialdehyde; MDA) levels of exercised rats compared with SC rats. The increased cardiac XO activity and MDA levels in exercised rats were significantly decreased in exercised rats supplemented with l-Arg. Myocardial GSSG content increased whereas the GSH:GSSG ratio was depressed in exercised rats compared with SC rats. Cardiac GSSG levels significantly decreased, whereas total glutathione, GSH and the GSH:GSSG ratio increased in exercised rats supplemented with l-Arg compared with exercised rats. The activities of creatinine kinase (CK) and lactate dehydrogenase (LDH), and lactate, uric acid, and nitrite and nitrate levels in the plasma significantly increased in exercised rats compared with SC rats. The activities of plasma CK and LDH were significantly decreased in l-Arg-supplemented plus exercised rats compared with exercised rats. These findings suggest that l-Arg supplementation reduces the oxidative damage and inflammatory response on the myocardium caused by exhaustive exercise in rats.

Keywords

L-ArginineXanthine oxidaseMyeloperoxidaseOxidative stressExhaustive exercise
Type
Research Article
Information
British Journal of Nutrition , Volume 95 , Issue 1 , January 2006 , pp. 67 - 75
Copyright
Copyright © The Nutrition Society 2006

References

Ashton, T, Rowlands, CC, Jones, E, Young, IS, Jackson, SK, Davies, B, Peters, JRElectron spin resonance spectroscopic detection of oxygen-centered radicals in human serum following exhaustive exercise. Eur J Appl Physiol Occup Physiol (1998) 77 498–502CrossRefGoogle Scholar
Au, A, Louch, WE, Ferrier, GR, Howlett, SEL-Arginine ameliorates effects of ischemia and reperfusion in isolated cardiac myocytes. Eur J Pharmacol (2003) 476 4554.CrossRefGoogle ScholarPubMed
Beckam, JS, Koppenol, WHNitric oxide, superoxide, and peroxynitrite: the good, the bad and ugly. Am J Physiol (1996) 271 C1424–C1437.Google Scholar
Beers, RFJr, Sizer, IWA spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem (1952) 195 133–140.CrossRefGoogle ScholarPubMed
Belcastro, AN, Arthur, GD,Albisser, TA, Raj, DAHeart, liver, and skeletal muscle myeloperoxidase activity during exercise. J Appl Physiol (1996) 80 1331–1335.CrossRefGoogle ScholarPubMed
Brooks, GAWhite, TPDetermination of metabolic and heart rate responses of rats to treadmill exercise. J Appl Physiol (1978) 45 1009–1015.CrossRefGoogle ScholarPubMed
de Oliveira, SLDiniz, DBAmaya-Farfan, JCarbohydrateenergy restriction may protect the rat brain against oxidative damage and improve physical performance. Br J Nutr (2003) 89 89–96.CrossRefGoogle ScholarPubMed
Duarte, JA, Appell, HJ,Carvalho, F, Bastos, ML, Soares, JMEndothelium-derived oxidative stress may contribute to exerciseinduced muscle damage. Int J Sports Med (1993) 14 440–443.CrossRefGoogle ScholarPubMed
Fielding, RA, Manfredi, T,Ding, W, Fiatarone, MA, Evans, WJ, Cannon, JGAcute phase response in exercise.III. Neutrophil and IL-1 beta accumulation in skeletal muscle. Am J Physiol (1993) 265 R166–R172.Google ScholarPubMed
Frankiewicz-Jozko, A, Faff, J, Sieradzan-Gabelska, BChanges in concentrations of tissue free radical marker and serum creatine kinase during the post-exercise period in rats. Eur J Appl Physiol (1996) 74 470474.CrossRefGoogle ScholarPubMed
Fukahori, M, Ichimori, k,Ishida, H, Nakagawa, H, Okino, HNitric oxide reversibly suppresses xanthine oxidase activity. Radic Res (1994) 21 203–212.CrossRefGoogle ScholarPubMed
Green, LC, Wagner, DA, Glogowski, J, Skipper, PL, Wishnok, JS, Tannenbaum, SRAnalysis of nitrate, nitrite and [15N]nitrate in biological fluids. Anal Biochem (1982) 126 131–138.CrossRefGoogle ScholarPubMed
Griffith, OWDetermination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine. Anal Biochem (1980) 106 207–212.CrossRefGoogle ScholarPubMed
Hassoun, PM, Yu, FS,Zulueta, JJ, White, AC, Lanzillo, JJEffect of nitric oxide and cell redox ststus on the regulation of endothelial cell xanthine dehydrogenase.Am J Physiol (1995) 268 L809–L817.Google Scholar
Hellsten, Y, Frandsen, U,Orthenblad, N, Sjodin, B, Richter, EAXanthine oxidase in human skeletal muscle following eccentric exercise: a role in inflammation. J Physiol (1997) 498 239–248.CrossRefGoogle ScholarPubMed
Ji, LLAntioxidant enzyme responds to exercise and aging. Med Sci Sports Exerc (1993) 25 225231.CrossRefGoogle Scholar
Ji, LLAntioxidants and oxidative stress in exercise. Proc Soc Exp Biol Med (1999) 222 283–292.CrossRefGoogle ScholarPubMed
Ji, LLExercise-induced modulation of antioxidant defense. Ann N Y Acad Sci (2002) 959 82–92.CrossRefGoogle ScholarPubMed
Ji, LL, Dillon, D, Wu, EMyocardial aging: antioxdant enzyme systems and related biochemical properties. Am J Physiol (1991) 261 R386–R392.Google Scholar
Ji, LL, Mitchell, EWEffects of Adriamycin on heart mitochondrial function in rested and exercised rats. Biochem Pharmacol (1994 47 877–885.Google ScholarPubMed
Judge, AR, Dodd, SLXanthine oxidase and activated neutrophils cause oxidative damage to skeletal muscle after contractile claudication. Am J Physiol (2004) 286 H252–H256.Google ScholarPubMed
Kihlstrom, M, Ojala, J, Salminen, ADecreased level of cardiac antioxidants in endurance-trained rats. Acta Physiol Scand (1989) 135 549–554.CrossRefGoogle ScholarPubMed
Kubes, P, Suzuki, M, Granger, DNModulation of PDF-induced leukocyte adherence and increased microvascular premeability. Am J Physiol (1990) 259 G859–G864.Google Scholar
Kubes, P, Suzuki, M, Granger, DNNitric oxide: an endogenous modulator of leukocyte adhesion. PNAS (1991) 88 46514655.CrossRefGoogle ScholarPubMed
Kumar, CT, Reddy, VK,Prasad, M, Thyagaraju, K, Reddanna, PDietary supplementation of vitmin E protects heart tissue from exercise-induced oxidant stress. Mol Cell Biochem (1992) 111 109–115.CrossRefGoogle Scholar
Lass, A, Suessenbacher, A,Wolkart, G, Mayer, B, Brunner, FFunctional and analytical evidence for scavenging of oxygen radicals by L-arginine. Mol Pharmacol (2002) 61 10811088.CrossRefGoogle ScholarPubMed
Leeuwenburgh, C, Leichtweis, S,Hollander, J, Fiebig, R, Groe, M, Ji, LLEffect of acute exercise on glutathione deficient heart. Mol Cell Biochem (1996) 156 17–24.CrossRefGoogle ScholarPubMed
Leichtweis, SB, Leeuwenburgh, C,Parmelee, DJ, Fiebig, R, Ji, LLRigorous swim training impairs mitochondrial function in post-ischaemic rat heart. Acta Physiol Scand (1997) 160 139–148.Google ScholarPubMed
Lewis, B, Langkamp-Henken, BArginine enhances in vivo immune responses in young, adult and aged mice. J Nutr (2000) 130 1827–1830.CrossRefGoogle ScholarPubMed
Liu, P, Hock, CE,Nagele, R, Wong, PYFormation of nitric oxide, superoxide, and peroxynitrite in myocardial ischemia-reperfusion-injury in rats. Am J Physiol (1997) 272 H2327–H2336.Google ScholarPubMed
Lowry, OH, Rosebrough, NJ,Lewis Farr, A, Randall, RJProtein measurement with the Folin phenol reagent. J Biol Chem (1951) 193 265–275.CrossRefGoogle ScholarPubMed
McCord, JMOxygen-derived free radicals in postischemic tissue injury. N Engl J Med (1985) 312, 159–163.Google ScholarPubMed
Mullane, KM, Kraemer, R, Smith, BMyeloperoxidase activity as a quantitative assessment of neutrophil infiltration into ischemicmyocardium. J Pharmacol Methods (1985) 14 157–167.CrossRefGoogle Scholar
Nonami, YThe role of nitric oxide in cardiac ischemia-reperfusion injury. Jpn Circ J (1997) 61 119–132.CrossRefGoogle ScholarPubMed
Pabla, R, Buda, AJ,Flynn, DM, Blesse, SA, Shin, AM, Curtis, MJ, Lefer, DJNitric oxide attenuates neutrophil-mediated myocardial contractile dysfunction after ischemia and reperfusion. Circ Res (1996) 78 65–72.CrossRefGoogle ScholarPubMed
Qian, ZM, Xiao, DS,Ke, Y, Liao, QKIncreased nitric oxide is one of the causes of changes of iron metabolism in strenuously exercised rats. Am J Physiol (2001) 280 R739–R743.Google ScholarPubMed
Radak, Z, Asano, K,Inoue, M, Kizaki, T, Oh-Ishi, S, Suzuki, K, Taniguchi, N, Ohno, HSuperoxide dismutase derivative reduces oxidative damage in skeletal muscle of rats during exhaustive exercise. J Appl Physiol (1995) 79 129–135.CrossRefGoogle ScholarPubMed
Radak, Z, Nakamura, A,Nakamoto, H, Asano, K, Ohno, H, Goto, SA period of anaerobic exercise increases the accumulation of reactive carbonyl derivatives in the lungs of rats. Pflugers Arch (1998) 435 439–441.Google ScholarPubMed
Reeves, PG, Nielsen, FH, Fahey, GC JrAIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr (1993) 123 1939–1951.CrossRefGoogle Scholar
Reid, MBRole of nitric oxide in skeletal muscle: Synthesis, distribution and functional importance. Acta Physiol Scand (1998) 162 401–409.CrossRefGoogle ScholarPubMed
Rubbo, H, Radi, R,Trujillo, M, Telleri, R, Kalyanaraman, B, Barnes, S, Kirk, M, Freeman, BANitric oxide regulation of superoxide and peroxylnitrite-dependent lipid peroxidation. Formation of novel nitrogen-containing oxidized lipid derivatives. Biol Chem (1994) 269 26066–26075.CrossRefGoogle ScholarPubMed
Schaefer, A, Piquard, F,Geny, B, Doutreleau, S, Lampert, E, Mettauer, B, Lonsdorfer, JL-Arginine reduces exercise-induced increase in plasma lactate and ammonia. Int J Sports Med (2002) 23 403–407.CrossRefGoogle ScholarPubMed
Schierwagen, C, Bylund-Fellenius, AC, Lundberg, CImproved method for quantification of tissue PMN accumulation measured by myeloperoxidase activity. J Pharmacol Methods (1990) 23 179–186.CrossRefGoogle ScholarPubMed
Schmidt, HH, Warner, TD,Nakane, M, Forstermann, U, Murad, FRegulation and subcellular location of nitrogen oxide synthases in RAW264.7 macrophages. Mol Pharmacol (1992) 41 615–624.Google ScholarPubMed
Schulz, R, Dodge, KL,Lopaschuk, GD, Clanachan, ASPeroxynitrite impairs cardiac contractile function by decreasing cardiac efficiency. Am J Physiol (1997) 272 H1212–H1219.Google ScholarPubMed
Sen, CKAtalay, MHanninen, OExercise-induced oxidative stress: glutathione supplementation and deficiency. J Appl Physiol (1994) 77 2177–2187.CrossRefGoogle ScholarPubMed
Seward, SW, Seiler, KS, Starnes, JWIntrinsic myocardial function and oxidative stress after exhaustive exercise. J Appl Physiol (1995) 79 251–255.CrossRefGoogle ScholarPubMed
Shiono, N, Rao, V, Weisel, RD, Kawasaki, M, Li, RK, Mickle, DAG, Fedak, PWM, Tumiati, LC, Ko, L, Verma, SL-Arginine protects human heart cells from low-volume anoxia and reoxygenation. Am J Physiol 2002) 282 H805–H815.Google ScholarPubMed
Somani, SM, Frank, S, Rybak, LPResponses of antioxidant system to acute and trained exercise in rat heart subcellular fractions. Pharmacol Biochem Behav (1995) 51 627–634.CrossRefGoogle ScholarPubMed
Tietze, FEnzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal Biochem (1969) 27 502–522.CrossRefGoogle ScholarPubMed
Tiidus, PMRadical species in inflammation and overtraining. Can J Physiol Pharmacol (1998) 76 533–538.CrossRefGoogle ScholarPubMed
Venditti, P, Di Meo, SAntioxidants, tissue damage, and endurance in trained and untrained young male rats. Arch Biochem. Biophys (1996) 331 63–68.Google Scholar
Vina, J, Gimeno, A,Sastre, J, Desco, C, Asensi, M, Pallardo, FV, Cuesta, A, Ferrero, JA, Terada, LS, Repine, JEMechanism of free radical production in exhaustive exercise in humans and rats; role of xanthine oxidase and protection by allopurinol. IUBMB Life (2000) 49 539–544.CrossRefGoogle ScholarPubMed
Wang, BY, Singer, AH,Tsao, PS, Drexler, H, Kosek, J, Cooke, JPDietary arginine prevents atherogenesis in the coronary artery of the hypercholesterolemic rabbit. J Am Coll Cardiol (1994) 23 452–458.CrossRefGoogle ScholarPubMed
Wascher, TC, Posch, K,Wallner, S, Hermetter, A, Kostner, GM, Graier, WFVascular effects of L-arginine: anything beyond a substrate for the NO-synthase?. Biochem Biophys Res Commun (1997) 234 35–38.CrossRefGoogle ScholarPubMed
Westerfeld, WW, Richert, DA, Higgins, ESFurther studies with xanthine oxidase inhibitors. J Biol Chem (1959) 234 1897 –1900.CrossRefGoogle ScholarPubMed
Westing, YHEkblom, BSjodin, BThe metabolic relation between hypoxanthine and uric acid in man following maximal short-distance running. Acta Physiol Scand (1989) 137 341–345.CrossRefGoogle ScholarPubMed
Wu, G, Morris, SM JrArginine metabolism: nitric oxide and beyond. Biochem J (1998) 336 1–17.CrossRefGoogle ScholarPubMed
Xiao, DS, Jiang, L,Che, LL, Lu, LNitric oxide and iron metabolism in exercised rat with L-arginine supplementation. Mol Cell Biochem (2003) 252 65–72.CrossRefGoogle ScholarPubMed
Yasmin, W, Strynadka, KD, Schulz, RGeneration of peroxynitrite contributes to ischemia-reperfusion injury in isolated rat hearts. Cardiovasc Res (1997) 33 422–432.CrossRefGoogle ScholarPubMed
Zweier, JL, Wang, P, Samouilov, A, Kuppusamy, PEnzymeindependent formation of nitric oxide in biological tissues. Nat Med (1995) 1 804–809.CrossRefGoogle ScholarPubMed