Hostname: page-component-78c5997874-j824f Total loading time: 0 Render date: 2024-11-10T15:59:30.582Z Has data issue: false hasContentIssue false
  • English
  • Français

Lipid peroxidation, prostacyclin and thromboxane A2 in pigs depleted of vitamin E and selenium and supplemented with linseed oil

Published online by Cambridge University Press:  09 March 2007

Maeve R. Nolan ,
Seamus Kennedy ,
W. John Blanchflower  and
D. Glenn Kennedy
Maeve R. Nolan
Affiliation:
Veterinary Sciences Division, Department of Agriculture for Northern Ireland, Stormont, Belfast BT4 3SD, Northern Ireland
Seamus Kennedy
Affiliation:
Veterinary Sciences Division, Department of Agriculture for Northern Ireland, Stormont, Belfast BT4 3SD, Northern Ireland
W. John Blanchflower
Affiliation:
Veterinary Sciences Division, Department of Agriculture for Northern Ireland, Stormont, Belfast BT4 3SD, Northern Ireland
D. Glenn Kennedy
Affiliation:
Veterinary Sciences Division, Department of Agriculture for Northern Ireland, Stormont, Belfast BT4 3SD, Northern Ireland
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

In a 2×2 balanced factorial experiment the biochemical effects on pigs of two dietary factors were investigated. The first factor was α-tocopherol and Se supplementation and the second factor was supplementation with α-tocopherol-stripped linseed oil. In pigs fed on diets depleted of α-tocopherol and Se, increases in concentrations of markers of lipid peroxidation (4-hydroxynonenal and hexanal) were observed. However, skeletal myopathy was only observed in those pigs fed on diets depleted of α-tocopherol and Se and supplemented with oil. In those pigs, increased lipid peroxidation was observed in heart and supraspinatus muscle. The plasma concentration of thromboxane B2 was increased in pigs fed on diets depleted of α-tocopherol and Se, suggesting an increased tendency towards platelet aggregation. However, this change was reversed in pigs depleted of α-tocopherol and Se, but supplemented with oil. This may have been a consequence of loss of arachidonic acid, the substrate for thromboxane formation, as a result of lipid peroxidation.

Keywords

Vitamin ELipid peroxidation4-HydroxynonenalProstacyclinThromboxane A2
Type
Lipid peroxidation and eicosanoid production in pigs
Information
British Journal of Nutrition , Volume 74 , Issue 3 , September 1995 , pp. 369 - 380
Copyright
Copyright © The Nutrition Society 1995

References

Bellisola, G., Galassini, S., Moschini, G., Poli, G., Perona, G. & Guidi, G. (1992). Selenium and glutathione peroxidase variations induced by polyunsaturated fatty acids oral supplementation in humans. Clinica et Chimica Acta 205, 75–85.CrossRefGoogle ScholarPubMed
Blanchflower, W. J., Walsh, D. M., Kennedy, S. & Kennedy, D. G. (1993). A thermospray mass spectrometric assay for Fe-induced 4-hydroxynonenal in tissues. Lipids 28, 261–264.CrossRefGoogle ScholarPubMed
Blood, D. C. & Radostis, O. M. (1990). Veterinary Medicine, pp. 1187–1202. London: Baillière Tindall.Google Scholar
Buttriss, J. L. & Diplock, A. T. (1988). The α-tocopherol and phospholipid fatty acid content of rat liver subcellular membranes in vitamin E and selenium deficiency. Biochimica et Biophysica Acta 963, 61–69.Google Scholar
Christie, W. W. (1989). Gas Chromatography and Lipids, pp. 64–128. Ayr, Scotland: The Oily Press.Google Scholar
Cunnane, S. C. (1988). Vitamin E intake affects serum thromboxane and tissue essential fatty acid composition in the rat. Annals of Nutrition and Metabolism 32, 90–96.CrossRefGoogle ScholarPubMed
Douglas, C. E., Chan, A. C. & Choy, P. C. (1986). Vitamin E inhibits platelet phospholipase A2. Biochimica et Biophysica Acta 876, 639–645.Google Scholar
Frankel, E. N., Hu, L. M. & Tappel, A. L. (1989). Rapid headspace gas chromatography of hexanal as a measure of lipid peroxidation in biological samples. Lipids 24, 976–981.Google Scholar
Gelman, A. L. (1985). Some studies with a Varian VGA-76 Hydride Generator for selenium determination. Varian Technical Notes no. AA-44. Mulgrave, Australia: Varian Instrument Group.Google Scholar
Karpen, C. W., Merola, A. J., Trewyn, R. W., Cornwell, D. G. & Panganamala, R. V. (1981). Modulation of platelet thromboxane A2 and arterial prostacyclin by dietary vitamin E. Prostaglandins 22, 651–661.CrossRefGoogle ScholarPubMed
McMurray, C. H. & Blanchflower, W. J. (1979). Determination of α-tocopherol in animal feedingstuffs using high performance liquid chromatography with spectrofluorescence detection. Journal of Chromatography 176, 488–492.CrossRefGoogle Scholar
McMurray, C. H., Blanchflower, W. J. & Rice, D. A. (1980). The effect of pre-treatment on the stability of alpha-tocopherol in moist barley. Proceedings of the Nutrition Society 39, 61 A.Google Scholar
Machlin, L. J. (1961). Destruction of vitamin E in cottonseed oil. Poultry Science 40, 1631–1632.CrossRefGoogle Scholar
Moncada, S. & Vane, J. R. (1979). Arachidonic acid metabolites and the interactions between platelets and blood-vessel walls. New England Journal of Medicine 300, 1142–1147.CrossRefGoogle ScholarPubMed
Nafstad, I. & Tollersrud, S. (1970). The vitamin E-deficiency syndrome in pigs. I. Pathological changes. Acta Veterinaria Scandinavica 11, 1–29.Google Scholar
Nielsen, T. K., Wolstrup, C., Schirmer, A. L. & Jensen, P. T. (1989). Mulberry heart disease in young pigs without vitamin E and selenium deficiency. Veterinary Record 124, 535–541.Google Scholar
Nolan, M., Kennedy, D. G., Blanchflower, W. J. & Kennedy, S. (1993). Indices of lipid peroxidation in the heart of vitamin E deficient pigs with or without supplementation with polyunsaturated fatty acids. Proceedings of the Nutrition Society 52, 81A.Google Scholar
Nolan, M. R., Kennedy, D. G., Blanchflower, W. J. & Kennedy, S. (1995). Feeding corn oil to vitamin E-deficient pigs increases lipid peroxidation and decreases tissue glutathione concentrations. International Journal of Vitamin and Nutrition Research (In the Press).Google Scholar
Rice, D. A. & Kennedy, S. (1989). Vitamin E, selenium, and polyunsaturated fatty acid concentrations and glutathione peroxidase activity in tissues from pigs with dietetic microangiopathy (mulberry heart disease). American Journal of Veterinary Research 50, 2101–2104.Google ScholarPubMed
Ruth, G. R. & Van Vleet, J. F. (1974). Experimentally induced selenium-vitamin E deficiency in growing swine: selective destruction of type I skeletal muscle fibers. American Journal of Veterinary Research 35, 237–244.Google ScholarPubMed
Valentovic, M. A., Gairola, C. & Lubawy, W. C. (1982). Lung, aorta, and platelet metabolism of 14C-arachidonic acid in vitamin E deficient rats. Prostaglandins 24, 215–224.Google Scholar
Van Vleet, J. F., Ferrans, V. J. & Ruth, G. R. (1977). Ultrastructural alterations in nutritional cardiomyopathy of selenium-vitamin E deficient swine II. Vascular lesions. Laboratory Investigations 37, 201–211.Google ScholarPubMed
Walsh, D. M., Kennedy, S., Blanchflower, W. J. & Kennedy, D. G. (1993). Vitamin E and selenium deficiency increase indices of lipid peroxidation in muscle tissue of ruminant calves. International Journal for Vitamin and Nutrition Research 63, 188–194.Google ScholarPubMed
Witting, L. A. & Horwitt, M. K. (1967). The effect of antioxidant deficiency on tissue lipid composition in the rat. I. Gastrocnemius and quadriceps muscle. Lipids 2, 89–96.Google Scholar