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Lycopene supplementation attenuated xanthine oxidase and myeloperoxidase activities in skeletal muscle tissues of rats after exhaustive exercise

Published online by Cambridge University Press:  08 March 2007

Chieh-Chung Liu ,
Chi-Chang Huang ,
Wan-Teng Lin ,
Chin-Cheng Hsieh ,
Shih-Yi Huang ,
Su-Jiun Lin  and
Suh-Ching Yang
Chieh-Chung Liu
Affiliation:
Department of Physical Education, Yuan Pei University of Science and Technology, Hsin Chu, Taiwan
Chi-Chang Huang
Affiliation:
School of Nutrition and Health Sciences, Taipei Medical University, Taipei, Taiwan
Wan-Teng Lin
Affiliation:
Department of Nutrition and Food Sciences, Fu-Jen Catholic University, Taipei, Taiwan De Lin Institute of Technology, Taipei, Taiwan
Chin-Cheng Hsieh
Affiliation:
Department of Physical Education, Yuan Pei University of Science and Technology, Hsin Chu, Taiwan
Shih-Yi Huang
Affiliation:
School of Nutrition and Health Sciences, Taipei Medical University, Taipei, Taiwan
Su-Jiun Lin
Affiliation:
Graduate Institute of Biology and Environment Science, School of Cellular and Molecular Biology, University of New Haven, CT 06516, USA
Suh-Ching Yang*
Affiliation:
School of Nutrition and Health Sciences, Taipei Medical University, Taipei, Taiwan
*
*Corresponding author: Dr Suh-Ching Yang, fax +886 2 2737 3112, email sokei@tmu.edu.tw
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Abstract

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Strenuous exercise is known to induce oxidative stress leading to the generation of free radicals. The purpose of the present study was to investigate the effects of lycopene, an antioxidant nutrient, at a relatively low dose (2·6 mg/kg per d) and a relatively high dose (7·8 mg/kg per d) on the antioxidant status of blood and skeletal muscle tissues in rats after exhaustive exercise. Rats were divided into six groups: sedentary control (C); sedentary control with low-dose lycopene (CLL); sedentary control with high-dose lycopene (CHL); exhaustive exercise (E); exhaustive exercise with low-dose lycopene (ELL); exhaustive exercise with high-dose lycopene (EHL). After 30 d, the rats in the three C groups were killed without exercise, but the rats in the three E groups were killed immediately after an exhaustive running test on a motorised treadmill. The results showed that xanthine oxidase (XO) activities of plasma and muscle, and muscular myeloperoxidase (MPO) activity in group E were significantly increased compared with group C. Compared with group E, the elevations of XO and MPO activities of muscle were significantly decreased in group EHL. The malondialdehyde concentrations of plasma and tissues in group E were significantly increased by 72 and 114 %, respectively, compared with those in group C. However, this phenomenon was prevented in rats of the ELL and EHL groups. There was no significant difference in the GSH concentrations of erythrocytes in each group; however, exhaustive exercise resulted in a significant decrease in the GSH content of muscle. In conclusion, these results suggested that lycopene protected muscle tissue from oxidative stress after exhaustive exercise.

Keywords

LycopeneXanthine oxidaseMyeloperoxidaseOxidative stressExhaustive exercise
Type
Research Article
Information
British Journal of Nutrition , Volume 94 , Issue 4 , October 2005 , pp. 595 - 601
Copyright
Copyright © The Nutrition Society 2005

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