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Lymphatic absorption of plasmalogen in rats

Published online by Cambridge University Press:  07 June 2007

Hiroshi Hara ,
Takuya Wakisaka  and
Yoritaka Aoyama
Hiroshi Hara*
Affiliation:
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kitaku, Sapporo 060-8589, Japan
Takuya Wakisaka
Affiliation:
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kitaku, Sapporo 060-8589, Japan
Yoritaka Aoyama
Affiliation:
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kitaku, Sapporo 060-8589, Japan
*
*Corresponding author: Dr Hiroshi Hara, fax +81 11 706 2504, email hara@chem.agr.hokudai.ac.jp
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Abstract

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Plasmalogen is a subclass of phospholipids that is widely distributed in man and animals. Many physiological roles have been proposed for this lipid; however, there have been no reports on the intestinal absorption of plasmalogen. In the present study, we examined lymphatic absorption of plasmalogen after the duodenal infusion of emulsified brain phospholipids (BPL) containing plasmalogen (22 mol% of total phospholipids) and soyabean lecithin (SPL) (100 g emulsified phospholipid/l). Male Wistar rats with implanted cannulas in the mesenteric lymph duct and the duodenum were kept in a Bollman-type restraining cage, and were infused the emulsion after 1 d recovery with duodenal infusion of a glucose–NaCl solution. Lymphatic plasmalogen output was increased at 2–4 h after the switch to BPL emulsion, and peaked at 4–6 h. However, no increases were observed after SPL infusion. Lymphatic recovery of plasmalogen for 8 h was 198 nmol, which was 0·22 mol% of the total plasmalogen disappeared from the intestine. We did not detect any increases in long-chain fatty aldehydes, which are the degradation product of plasmalogen, either in the blood or the small intestine. We conclude that a small percentage but a significant amount of the plasmalogen was absorbed into the lymph.

Keywords

PlasmalogenPhospholipidsLymphatic absorptionRats
Type
Research Article
Information
British Journal of Nutrition , Volume 90 , Issue 1 , July 2003 , pp. 29 - 32
Copyright
Copyright © The Nutrition Society 2003

References

American Institute of Nutrition (1977) Report of the American Institute of Nutrition ad hoc Committee on Standards for Nutritional Studies. J Nutr 107, 1340–1348.Google Scholar
American Institute of Nutrition (1980) Second report of the ad hoc Committee on Standards for Nutritional Studies. J Nutr 110, 1726Google Scholar
Blank, ML, Cress, EA, Smith, ZL & Snyder, F (1992) Meats and fish consumed in the American diet contain substantial amounts of ether-linked phospholipids. J Nutr 122, 1656–1661.Google Scholar
Bollman, JL, Cain, JC & Grindlay, JH (1948) Techniques for the collection of lymph from the liver, small intestine, or thoracic duct of the rat. J Lab Clin Med 33, 1349–1352.Google Scholar
Duncan, DB (1995) Multiple range and multiple F tests. Biometrics 11, 1–42.Google Scholar
Engelmann, B, Brautigam, C & Thiery, J (1994) Plasmalogen phospholipids as potential protectors against lipid peroxidation of low density lipoproteins. Biochem Biophys Res Commun 204, 1235–1242.Google Scholar
Farooqui, AA, Rapoport, SI & Horrocks, LA (1997) Membrane phospholipid alterations in Alzheimer's disease: deficiency of ethanolamine plasmalogens. Neurochem Res 22, 523–527.Google Scholar
Folch, J (1942) Brain cephalin, a mixture of phosphatides. Separation from it of phosphatdylserine, phosphatidylethanolamine, and a fraction containing an inositol phosphatides. J Biol Chem 146, 35–44.Google Scholar
Folch, J, Lees, M & Sloane-Stanley, GH (1957) A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 226, 497–509.Google Scholar
Glaser, PE & Gross, RW (1994) Plasmenylethanolamine facilitates rapid membrane fusion: a stopped-flow kinetic investigation correlating the propensity of a major plasma membrane constituent to adopt an HII phase with its ability to promote membrane fusion. Biochemistry 33, 5805–5812.CrossRefGoogle Scholar
Gottfried, EL & Rapport, MM (1962) The biochemistry of plasmalogens. I. Isolation and characterization of phosphatidal choline, a pure native plasmalogen. J Biol Chem 237, 329–333.Google Scholar
Hahnel, D, Beyer, K & Engelmann, B (1999) Inhibition of peroxyl radical-mediated lipid oxidation by plasmalogen phospholipids and alpha-tocopherol. Free Radic Biol Med 27, 1087–1094.Google Scholar
Huque, T, Brand, JG, Rabinowitz, JL & Gavarron, FF (1987) A comparative study of three methods for the estimation of total plasmalogens in lingual taste epithelium and other tissues. Comp Biochem Physio B 86B, 135–139.Google Scholar
Jurgens, G, Fell, A, Ledinski, G, Chen, Q & Paltauf, F (1995) Delay of copper-catalyzed oxidation of low density lipoprotein by in vitro enrichment with choline or ethanolamine plasmalogens. Chem Phys Lipids 77, 25–31.Google Scholar
Jurkowitz, MS, Horrocks, LA & Litsky, ML (1999) Identification and characterization of alkenyl hydrolase (lysoplasmalogenase) in microsomes and identification of a plasmalogen-active phospholipase A2 in cytosol of small intestinal epithelium. Biochim Biophys Acta 1437, 142–156.Google Scholar
Jurkowitz-Alexander, M, Ebata, H, Mills, JS, Murphy, EJ & Horrocks, LA (1989) Solubilization, purification and characterization of lysoplasmalogen alkenylhydrolase (lysoplasmalogenase) from rat liver microsomes. Biochim Biophys Acta 1002, 203–212.Google Scholar
Nagan, N & Zoeller, RA (2001) Plasmalogens: biosynthesis and functions. Prog Lipid Res 40, 199–229.Google Scholar
Pries, C & Böttcher, CJF (1965) The determination of free and plasmalogen-bound aldehydes in lipid fraction. Biochim Biophys Acta 98, 329–334.Google Scholar
Stadelmann-Ingrand, S, Favreliere, S, Fauconneau, B, Mauco, G & Tallineau, C (2001) Plasmalogen degradation by oxidative stress: Production and disappearance of specific fatty aldehydes and fatty alpha-hydroxyaldehydes. Free Radic Biol Med 31, 1263–1271.Google Scholar
Williams, JNJ, Anderson, CE & Jasik, AD (1962) A sensitive and specific method for plasmalogens and other enol esters. J Lipid Res 3, 378–381.Google Scholar
Zoeller, RA, Lake, AC, Nagan, N, Gaposchkin, DP, Legner, MA & Lieberthal, W (1999) Plasmalogens as endogenous antioxidants: somatic cell mutants reveal the importance of the vinyl-ether. Biochem J 338, 769–776.Google Scholar
Zoeller, RA, Morand, OH & Raetz, CR (1988) A possible role for plasmalogens in protecting animal cells against photosensitized killing. J Biol Chem 263, 11590–11596.Google Scholar
Zommara, M, Tachibana, N & Mitsui, K, et al. (1995) Inhibitory effect of ethanolamine plasmalogen on iron- and copper-dependent lipid peroxidation. Free Radic Biol Med 18, 599–602.Google Scholar