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Lysine requirements and whole-body protein turnover in growing pigs

Published online by Cambridge University Press:  09 March 2007

D. N. Salter ,
A. I. Montgomery ,
Anna Hudson ,
D. B. Quelch  and
Rosemary J. Elliott
D. N. Salter
Affiliation:
AFRC Institute for Grassland and Animal Production, Shinjeld Research Station, Church Lane, Shinzeld, Reading RG2 9AQ, Berks.
A. I. Montgomery
Affiliation:
AFRC Institute for Grassland and Animal Production, Shinjeld Research Station, Church Lane, Shinzeld, Reading RG2 9AQ, Berks.
Anna Hudson
Affiliation:
AFRC Institute for Grassland and Animal Production, Shinjeld Research Station, Church Lane, Shinzeld, Reading RG2 9AQ, Berks.
D. B. Quelch
Affiliation:
AFRC Institute for Grassland and Animal Production, Shinjeld Research Station, Church Lane, Shinzeld, Reading RG2 9AQ, Berks.
Rosemary J. Elliott
Affiliation:
AFRC Institute for Grassland and Animal Production, Shinjeld Research Station, Church Lane, Shinzeld, Reading RG2 9AQ, Berks.
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Abstract

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The influence on protein accretion and whole-body protein turnover of changing dietary protein quality while maintaining constant energy intake was studied by varying the degree of lysine supplementation of a lysine-deficient barley-based diet given to growing pigs. Measurements of nitrogen metabolism and whole-body protein turnover, using both classical and 15N end-product methods following a single dose of lsqb;15N]glycine, were made in 49-kg male pigs given diets containing 109 g lysine-deficient protein/kg supplemented to make them (1) 'deficient', (2) 'adequate' and (3) 'in excess' with respect to lysine. The 15N dose and protein intake values used to calculate amino N flux from the cumulative urinary excretion of 15N in urea and ammonia were corrected respectively for apparent digestibilities of [15N]glycine and total N determined in a separate experiment in pigs fitted with simple ileal cannulas. N retention and biological value were significantly increased by lysine supplementation of the deficient diet to the 'adequate' level, but were not further increased by the higher level of supplementation. Rates of growth paralleled these changes. The poorer biological value of the unsupplemented diet 1 was shown also in a significantly higher excretion of urea N compared with diets 2 and 3. N digestibility was not markedly influenced by the level of lysine supplementation. Both whole-body protein synthesis and degradation increased markedly on 'adequate' supplementation of the diet with lysine, but did not increase further with an excess of lysine. It is concluded that the increase in protein accretion rate observed on supplementation of the diet with lysine was due to a greater increase in the rate of protein synthesis than of degradation, rather than a decrease in degradation rate.

Keywords

GrowthLysine requirementProtein turnoverPig
Type
Protein Nutrition and Metabolism
Information
British Journal of Nutrition , Volume 63 , Issue 3 , May 1990 , pp. 503 - 513
Copyright
Copyright © The Nutrition Society 1990

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