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The measurement of exchangeable pools of zinc using the stable isotope 70Zn

Published online by Cambridge University Press:  09 March 2007

Susan J. Fairweather-Tait ,
Malcolm J. Jackson ,
Thomas E. Fox ,
S. Gabrielle Wharf ,
John Eagles  and
Peter C. Croghan
Susan J. Fairweather-Tait
Affiliation:
AFRC Institute of Food Research, Norwich Laboratory, Norwich Research Park, Colney, Norwich NR4 7UA
Malcolm J. Jackson
Affiliation:
Department of Medicine, University of Liverpool, Liverpool L69 3BX
Thomas E. Fox
Affiliation:
AFRC Institute of Food Research, Norwich Laboratory, Norwich Research Park, Colney, Norwich NR4 7UA
S. Gabrielle Wharf
Affiliation:
AFRC Institute of Food Research, Norwich Laboratory, Norwich Research Park, Colney, Norwich NR4 7UA
John Eagles
Affiliation:
AFRC Institute of Food Research, Norwich Laboratory, Norwich Research Park, Colney, Norwich NR4 7UA
Peter C. Croghan
Affiliation:
School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ
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Abstract

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The present study was designed to assess the feasibility of using small doses of a stable isotope of Zn to follow plasma kinetics over a 10 d period and, hence, make deductions about Zn turnover and body pool sizes. At the beginning of the 10 d metabolic balance, two adults, consuming their habitual diet, were given an intravenous injection of 70Zn. There was a fourfold difference in the administered dose between the two subjects (0·445 and 2·078 mg). Blood samples were taken at regular intervals and plasma enrichment with 70Zn measured by thermal ionization mass spectrometry. Urine and faeces were collected and analysed for Zn and 70Zn. Kinetic analysis of the plasma 70Zn decay by several different methods was undertaken. It was apparent from both deconvolution analysis of the short-term (0–90 min) decay data and four-compartment modelling of the longer-term (0–24 h) data that isotopic Zn very rapidly equilibrates with the plasma Zn and with a rapidly exchanging non-plasma pool, probably located within the liver. This latter pool appears to contain less than 10 mg Zn and the peak of isotope enrichment occurs at about 20 min post injection. The later decay of plasma Zn enrichment appears to be dictated by exchange with a much larger pool of approximate size 350 mg.

Keywords

Exchangeable zinc poolsStable isotopesHuman subjectsZinc
Type
Exchangeable Pools of Zinc
Information
British Journal of Nutrition , Volume 70 , Issue 1 , July 1993 , pp. 221 - 234
Copyright
Copyright © The Nutrition Society 1993

References

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