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Mechanisms involved in the antiplatelet effect of C-phycocyanin

Published online by Cambridge University Press:  08 March 2007

Hui-Fen Chiu ,
Shih-Ping Yang ,
Yu-Ling Kuo ,
Yuan-Shu Lai  and
Tz-Chong Chou
Hui-Fen Chiu
Affiliation:
Department of Pharmacology, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan, Republic of China
Shih-Ping Yang
Affiliation:
Division of Cardiology, Tri-Service General Hospital, Taipei, Taiwan, Republic of China
Yu-Ling Kuo
Affiliation:
Department of Physiology and Biophysics, National Defense Medical Center, No. 161, Min-Chuan E. Rd., Sec. 6, Taipei, Taiwan, Republic of China
Yuan-Shu Lai
Affiliation:
Intensive Care Unit, Chung-Hsiao Municipal Hospital Taipei-City, Taipei, Taiwan, Republic of China
Tz-Chong Chou*
Affiliation:
Department of Physiology and Biophysics, National Defense Medical Center, No. 161, Min-Chuan E. Rd., Sec. 6, Taipei, Taiwan, Republic of China
*
*corresponding author: Dr Tz-Chong Chou, fax +886 2 8792 7202, email tcchou@ms5.hinet.net
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Abstract

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C-phycocyanin (cpc), a biliprotein isolated from Spirulina platensis, has been reported to exert many therapeutic and nutritional values. In the present study, we examined whether cpc has an antiplatelet activity in vitro and further investigated the possible anti-aggregatory mechanisms involved. Our results showed that preincubation of cpc (1–50μg/ml) with rabbit washed platelets dose-dependently inhibited the platelet aggregation induced by collagen (10μg/ml) or arachidonic acid (100μm), with an ic50 of about 10μg/ml. Furthermore, the thromboxane B2 formation caused by collagen or arachidonic acid was significantly inhibited by cpc due to suppression of cyclooxygenase and thromboxane synthase activity. Similarly, the rise of platelet intracellular calcium level stimulated by arachidonic acid and collagen-induced platelet membrane surface glycoprotein IIb/IIIa expression were also attenuated by cpc. In addition, cpc itself significantly increased the platelet membrane fluidity and the cyclic AMP level through inhibiting cyclic AMP phosphodiesterase activity. These findings strongly demonstrate that cpc is an inhibitor of platelet aggregation, which may be associated with mechanisms including inhibition of thromboxane A2 formation, intracellular calcium mobilization and platelet surface glycoprotein IIb/IIIa expression accompanied by increasing cyclic AMP formation and platelet membrane fluidity.

Keywords

C-phycocyaninPlatelet aggregationThromboxaneB2Cyclic AMP
Type
Research Article
Information
British Journal of Nutrition , Volume 95 , Issue 2 , February 2006 , pp. 435 - 440
Copyright
Copyright © The Nutrition Society 2006

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