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Mechanisms of intestinal phosphate transport in small ruminants

Published online by Cambridge University Press:  09 March 2007

B. Schröder ,
H. Käppner ,
K. Failing  and
E. Pfeffer
B. Schröder
Affiliation:
Department of Veterinary Physiology, Justus-Liebig-University, Giessen, Germany
H. Käppner
Affiliation:
Department of Veterinary Physiology, Justus-Liebig-University, Giessen, Germany
K. Failing
Affiliation:
Department of Veterinary Physiology, Justus-Liebig-University, Giessen, Germany
E. Pfeffer
Affiliation:
Department of Animal Nutrition, Rheinische Friedrich-Wilhelms-University, Bonn, Germany
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Abstract

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In order to study the localization and mechanisms of intestinal phosphate transport in sheep and goats, unidirectional inorganic phosphate (Pi) flux rates across isolated stripped epithelial tissues were measured in vitro by applying the Ussing-chamber technique. In the first experiment the tissues were obtained from animals which had been kept on an adequate dietary P supply. In the second experiment the animals had either been kept on an adequate Ca and P supply or were Ca- and/or P-depleted. Significant net Pi absorption was measured in all segments of the small intestine and in the proximal colon of sheep and in the duodenum and jejunum of goats. Since the experiments were carried out in the absence of any electrochemical gradient, this clearly indicates the presence of active mechanisms for Pi transport in the intestinal tract of small ruminants. In sheep jejunum, reduction of mucosal Na concentration to 1·8 mM or serosal application of ouabain (0·1 mM) resulted in significant decreases of net F; absorption of the same order of magnitude, indicating that about 65% of active Pi transport in sheep jejunum is mediated by a Na-dependent active transport mechanism. The mechanism for the remaining Na+-independent active Pi transport has not yet been identified. Dietary P depletion caused hypophosphataemia and induced a significant stimulation of net Pi absorption in goat duodenum and jejunum. This increase was independent of dietary Ca supply and was not associated with increased plasma calcitriol concentrations. This suggests substantial differences in hormonal regulation of Pi transport in small ruminants in comparison with single-stomached species.

Keywords

Intestinal phosphate absorptionPhosphorusCalciumRuminant
Type
Intestinal phosphate transport
Information
British Journal of Nutrition , Volume 74 , Issue 5 , November 1995 , pp. 635 - 648
Copyright
Copyright © The Nutrition Society 1995

References

Baxter, L. A. & DeLuca, H. F. (1976). Stimulation of 25-hydroxyvitamin D3-lα-hydroxylase by phosphate depletion. Journal of Biological Chemistry 251, 3158–3161.CrossRefGoogle Scholar
Baylink, D., Wergedal, J. & Stauffer, M. (1971). Formation, mineralization and resorption of bone in hypophosphatemic rats. Journal of Clinical Investigation 50, 2519–2530.CrossRefGoogle ScholarPubMed
Ben-Ghedalia, D., Tagari, H., Zamwel, S. & Bondi, A. (1975). Solubility and net exchange of calcium, magnesium and phosphorus in digesta flowing along the gut of the sheep. British Journal of Nutrition 33, 87–94.CrossRefGoogle ScholarPubMed
Berner, W., Kinne, R. & Murer, H. (1976). Phosphate transport into brush-border membrane vesicles isolated from rat small intestine. Biochemical Journal 160, 467–474.CrossRefGoogle ScholarPubMed
Blaustein, M. P. (1993). Physiological effects of endogenous ouabain: control of intracellular Ca2+ stores and cell responsiveness. American Journal of Physiology 264, C1367–C1387.CrossRefGoogle ScholarPubMed
Brandis, M., Harmeyer, J., Kaune, R., Mohrmann, M., Murer, H. & Zimolo, Z. (1987). Phosphate transport in brush-border membranes from control and rachitic pig kidney and small intestine. Journal of Physiology 384,479–490.CrossRefGoogle ScholarPubMed
Breves, G., Ross, R. & Höller, H. (1985). Dietary phosphorus depletion in sheep: effects on plasma inorganic phosphorus, calcium, 1,25(OH)2-Vit-D3 and alkaline phosphatase and on gastrointestinal P and Ca balances. Journal of Agricultural Science, Cambridge 105, 623–629.CrossRefGoogle Scholar
Breves, G. & Schröder, B. (1991). Comparative aspects of gastrointestinal phosphorus metabolism. Nutrition Research Reviews 4, 125–140.CrossRefGoogle ScholarPubMed
Brommage, R., Baxter, D. C. & Gierke, L. W. (1990). Vitamin D-independent intestinal calcium and phosphorus absorption during reproduction. American Journal of Physiology 259, G631—G638.Google ScholarPubMed
Bruce, J., Goodall, E., Kay, R., Phillipson, A. & Vowles, L. (1966/1967). The flow of organic and inorganic materials through the alimentary tract of the sheep. Proceedings of the Royal Society 166B, 46–62.Google Scholar
Care, A., Barlet, J. & Abdel-Hafeez, H. (1980). Calcium and phosphate homeostasis in ruminants and its relationship to the aetiology and prevention of parturient paresis. In Digestive Physiology and Metabolism in Ruminants, pp. 429–446 [Ruckebusch, Y. and Thivend, P., editors]. Lancaster: MTP Press Ltd.CrossRefGoogle Scholar
Caverzasio, J., Danisi, G., Murer, H. & Bonjour, J. (1987). Adaptation of phosphate transport to low phosphate diet in renal and intestinal brush border membrane vesicles: influence of sodium and pH. European Journal of Physiology - Pflüsers Archiv 409, 333–336.CrossRefGoogle ScholarPubMed
Chu, L. L. H., McGregor, R. R., Anast, C. S., Hamilton, J. W. & Cohn, D. V. (1973). Studies on the biosynthesis of rat parathyroid hormone and proparathyroid hormone: adaptation of the parathyroid gland to dietary restriction of calcium. Endocrinology 93, 915–924.CrossRefGoogle ScholarPubMed
Cross, H., Debiec, H. & Peterlik, M. (1990). Mechanism and regulation of intestinal phosphate absorption. Mineral and Electrolyte Metabolism 16, 115–124.Google ScholarPubMed
Cross, H. S., Pölzleitner, D. & Peterlik, M. (1986). Intestinal phosphate and calcium absorption: joint regulation by thyroid hormones and 1,25-dihydroxyvitamin D3. Acta Endocrinologica 113, 96–103.Google Scholar
Daniel, H., Fett, C., Kratz, A. & Rehner, G. (1989). Demonstration and modification of intervillous pH profiles in the rat jejunum in vitro. American Journal of Physiology 257, G489–G495.Google ScholarPubMed
Danisi, G., Caverzasio, J., Trechsel, U., Bonjour, J. & Straub, R. (1990). Phosphate transport adaptation in rat jejunum and plasma level of 1,25-dihydroxyvitamin-D3. Scandinavian Journal of Gastroenterology 25,210–215.CrossRefGoogle Scholar
Danisi, G., Caverzasio, J., Trechsel, U., Straub, R. & Bonjour, J. (1988). Phosphate transport adaptation inintestinal brush border membrane vesicles (BBMV) and plasma levels of 1,25-dihydroxycholecalciferol. In Cellular Calcium and Phosphate Transport in Health and Disease, pp. 65–66 [Bronner, F. and Peterlik, M., editors]. New York: Alan R. Liss, Inc.Google Scholar
Danisi, G. & Murer, H. (1991). Inorganic phosphate absorption in small intestine. In Handbook of Physiology. The Gastrointestinal System IV, pp. 323–336 [Schultz, S. G. editor]. New York: Oxford University Press.Google Scholar
Danisi, G., van Os, C. & Straub, R. (1984). Phosphate transport across brush border and basolateral membrane vesicles of small intestine. In Epithelial Calcium and Phosphate Transport: Molecular and Cellular Aspects, pp. 229–234 [Bronner, F. and Peterlik, M., editors]. New York: Alan R. Liss, Inc.Google Scholar
Dixon, W. J. (1987). BMDP Statistical Software Manual. Los Angeles: University of California Press.Google Scholar
Ferrante, P. L., Freeman, D. E., Whitlock, R. H. & Kronfeld, D. S. (1988). Effect of D-glucose on in vitro short-circuit current in neonatal calf jejunum and rabbit ileum. American Journal of Veterinary Research 49, 715–719.Google ScholarPubMed
Fox, J. (1992). Hypocalcemia, but not PTH or hypophosphatemia, induces a rapid increase in 1,25(OH)2,D3 levels in rats. American Journal of Physiology 262, E211–E215.Google ScholarPubMed
Fox, J. & Care, A. D. (1976). The effects of hydroxylated derivatives of vitamin D3, and of extracts of Solanum malacoxylon on the absorption of calcium, phosphate and water from the jejunum of pigs. Calcified Tissue Research 21, Suppl., 147–152.CrossRefGoogle ScholarPubMed
Fox, J. & Care, A. D. (1978). Effect of low calcium and low phosphorus diets on the intestinal absorption of phosphate in intact and parathyroidectomized pigs. Journal of Endocrinology 77, 225–231.CrossRefGoogle ScholarPubMed
Fuchs, R. & Peterlik, M. (1980). Vitamin D-induced phosphate transport in intestinal brush border membrane vesicles. Biochemical and Biophysical Research Communications 93, 87–92.CrossRefGoogle ScholarPubMed
Grace, N. D., Ulyatt, M. J. & Macrae, J. C. (1974). Quantitative digestion of fresh herbage by sheep. III. The movement of Mg, Ca, P, K and Na in the digestive tract. Journal of Agricultural Science, Cambridge 82, 321–330.CrossRefGoogle Scholar
Gray, R. (1987). Evidence that somatomedins mediate the effect of hypophosphatemia to increase serum 1,25-dihydroxyvitamin D3 levels in rats. Endocrinology 121, 504–512.CrossRefGoogle ScholarPubMed
Gray, R. W., Garthwaite, T. L. & Phillips, L. S. (1983). Growth hormone and triiodothyronine permit an increase in plasma 1,25(OH)2D concentrations in response to dietary phosphate deprivation in hypophysectomized rats. Calcified Tissue International 35, 100–106.CrossRefGoogle Scholar
Hildmann, B., Storelli, C., Danisi, G. & Murer, H. (1982). Regulation of Na+-P1 cotransport by 1,25-dihydroxyvitamin D3 in rabbit duodenal brush-border membrane. American Journal of Physiology 242, G53–G539.Google Scholar
Hoffmann, N., Thees, M. & Kinne, R. (1976). Phosphate transport by isolated renal brush border vesicles. European Journal of Physiology - Pflugers Archiv 362, 147–156.CrossRefGoogle ScholarPubMed
Hollis, B. (1986). Assay of circulating 1,25-dihydroxyvitamin D involving a novel single-cartridge extraction and purification procedure. Clinical Chemistry 32, 2060–2062.CrossRefGoogle Scholar
Jungbluth, H. & Binswanger, U. (1989). Unidirectional duodenal and jejunal calcium and phosphorus transport in the rat: effects of dietary phosphorus depletion, ethane-1-hydroxy-1, 1-diphosphonate and 1,25-dihydroxycholecalciferol. Research in Experimental Medicine 189, 439–449.CrossRefGoogle Scholar
McEwan, G. T. A., Schousboe, B. & Skadhauge, E. (1990). Direct measurement of surface pH of pig jejunum in vivo. Journal of Veterinary Medicine A 37, 439–444.CrossRefGoogle ScholarPubMed
Matsumoto, T., Fontaine, O. & Rasmussen, H. (1980). Effect of 1,25-dihydroxyvitamin D3 on phosphate uptake into chick intestinal brush border membrane vesicles. Biochimica et Biophysica Acta 599, 13–23.CrossRefGoogle Scholar
Maunder, E., Pillay, A. & Care, A. (1986). Hypophosphatemia and vitamin D metabolism in sheep. Quarterly Journal of Experimental Physiology 71, 391–399.CrossRefGoogle ScholarPubMed
Murer, H. & Hildmann, B. (1981). Transcellular transport of calcium and inorganic phosphate in the small intestinal epithelium. American Journal of Physiology 240, G409–G416.Google ScholarPubMed
Peterlik, M. & Wasserman, R. H. (1980). Regulation by vitamin D of intestinal phosphate absorption. Hormone and Metabolic Research 12, 216–219.CrossRefGoogle ScholarPubMed
PeterlikM,. M,., Fuchs, R. & Cross, H. S. (1981). Phosphate transport in the intestine: cellular pathways and hormonal regulation. In Calcium and Phosphate Transport Across Biomembranes, pp. 173–179 [Bronner, F. and Peterlik, M., editors]. New York: Academic Press, Inc.CrossRefGoogle Scholar
Pfeffer, E., Thompson, A. & Armstrong, D. G. (1970). Studies on intestinal digestion in the sheep. 3. Net movement of certain inorganic elements in the digestive tract on rations containing different proportions of hay and rolled barley. British Journal of Nutrition 24, 197–204.CrossRefGoogle ScholarPubMed
Quamme, G. A. (1985). Phosphate transport in intestinal brush-border membrane vesicles: effect of pH and dietary phosphate. American Journal of Physiology 249, G168–G176.Google ScholarPubMed
Reinhardt, T. A. & Horst, R. L. (1990). Competitive protein-binding assay of calcitriol with an advanced preparation of bovine calf thymus cytosol. In Calcium Regulating Hormones, Vitamin D Metabolites, and Cyclic AMP. Assays and their Clinical Application, pp. 334—345 [Schmidt-Gayk, H., Armbruster, F. P. and Bouillon, R., editors]. Berlin, Heidelberg: Springer Verlag.Google Scholar
Sachs, L. (1992). Angewandte Statistik. Berlin, Heidelberg, New York: Springer Verlag.CrossRefGoogle Scholar
Scharrer, E. (1985). Phosphate absorption at different sites in the developing lamb. Quarterly Journal of Experimental Physiology 70, 615–621.CrossRefGoogle ScholarPubMed
Schneider, K. M., Straub, R. W. & Danisi, G. (1985a). Phosphate transport in the gut of the ruminant. Experientia 41, 834–835.Google Scholar
Schneider, K. M., Straub, R. W. & Danisi, G. (1985b). Sodium-phosphate cotransport in ruminant intestine. Mineral and Electrolyte Metabolism 11, 339.Google Scholar
Schröder, B., Breves, G. & Pfeffer, E. (1990). Binding properties of duodenal 1,25-dihydroxyvitamin D3, receptors as affected by phosphorus depletion in lactating goats. Comparative Biochemistry and Physiology 96A, 495–498.CrossRefGoogle Scholar
Schröder, B., Kaune, R. & Harmeyer, J. (1991). Effects of calcitriol on stimulation of ion transport in pig jejunal mucosa. Journal of Physiology 433, 451–465.CrossRefGoogle ScholarPubMed
Schröder, B., Kaune, R., Schlumbohm, C., Breves, G. & Harmeyer, J. (1993a). Evidence for vitamin-D-independent active calcium absorption in newborn piglets. Calcified Tissue International 52, 305–309.CrossRefGoogle ScholarPubMed
Schröder, B., Rübelt, A. & Breves, G. (1993b). Sodium coupled phosphate transport across jejunal brush border membrane from small ruminants. Biological Chemistry Hoppe-Seyler 374, 157.Google Scholar
Schultz, S. & Zalusky, R. (1964). Ion transport in isolated rabbit ileum. I. Short-circuit current and Na fluxes. Journal of General Physiology 47, 567–584.CrossRefGoogle ScholarPubMed
Scott, D., McLean, A. F. & Buchan, W. (1984). The effects of intravenous phosphate loading on salivary phosphorus secretion, net intestinal phosphorus absorption and fecal endogenous phosphorus excretion in sheep. Quarterly Journal of Experimental Physiology 69, 453–461.CrossRefGoogle Scholar
Shirazi-Beechey, S., Gorvel, J. & Beechey, R. (1988). Phosphate transport in intestinal brush-border membrane. Journal of Bioenergetics and Biomembranes 20, 273–288.CrossRefGoogle ScholarPubMed
Shirazi-Beechey, S. P., Beechey, R. B., Penny, J., Vayro, S., Buchan, W. & Scott, D. (1991). Mechanisms of phosphate transport in sheep intestine and parotid gland - response to variation in dietary phosphate supply. Experimental Physiology 16, 231–241.CrossRefGoogle Scholar
Shirazi-Beechey, S. P., Kemp, R. B., Dyer, J. & Beechey, R. B. (1989). Changes in the function of the intestinal brush border membrane during the development of the ruminant habit in lambs. Comparative Biochemistry and Physiology B94, 801–806.Google Scholar
Szczepanska-Konkel, M., Yusufi, A. N. K., VanScoy, M., Webster, S. K. & Dousa, T. P. (1986). Phosphono-carboxylic acids as specific inhibitors of Na+-dependent transport of phosphate across renal brush border membrane. Journal of BiologicaI Chemistry 261, 6375–6383.CrossRefGoogle Scholar
Wehrle, J. P. & Pedersen, P. L. (1989). Phosphate transport processes in eukaryotic cells. Journal of Membrane Biology 111, 199–213.CrossRefGoogle ScholarPubMed