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Metabolic fates of U-14C-labelled monosaccharides and an enzyme-treated cell-wall substrate in the fowl

Published online by Cambridge University Press:  09 March 2007

C. J. Savory
C. J. Savory
Affiliation:
AFRC Institute of Animal Physiology and Genetics Research, Roslin, Midlothian EH25 9PS
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Abstract

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A major benefit of supplementing non-ruminant feedstuffs with exogenous enzymes is presumed to be the degradation of plant cell-wall polysaccharides to metabolizable monosaccharide residues. In the present study, metabolic fates of (U-14C-labelled, 10 mM) glucose, galactose, mannose, xylose and arabinose were compared in the fowl, by measuring recoveries of 14C radioactivity in exhaled carbon dioxide, excreta and body tissues after administration either by wing vein (iv) or into the crop (ic). A further comparison was made with a tube-fed, enzyme-treated, U-14C-labelled cell-wall substrate, Festuca arundinacea and a final experiment tested possible competition for absorption between different cell-wall residues. Delays between iv and is treatments in recovery of 14C in CO2, which were assumed to reflect intestinal absorption, indicated that xylose was absorbed more slowly than glucose and galactose, but faster than mannose and arabinose. Total recoveries of 14C in CO2 and excreta over the whole test period indicated that metabolizabilities were highest with glucose, galactose and mannose, and lowest with arabinose. After testing, 14C recovery in caecal contents was highest with is arabinose, and recoveries in body tissues, with all sugar treatments, were in the order liver > breast and leg muscle > abdominal fat > plasma. Results with the Festuca substrate showed similar patterns of recovery in body tissues and confirmed an increase in metabolizability with addition of enzymes. The timing of the 14CO2 response with Festuca and a wet enzyme pretreatment was broadly similar to a ‘predicted Festuca’ response, based on the composition of the substrate and the measured responses with individual (ic) monosaccharides. There was no evidence of any competition for absorption or metabolism among cell-wall residues. It was concluded that glucose release from cellulose is potentially the most important product of cell-wall degradation to contribute to enzyme enhancement of metabolizable energy.

Keywords

Cell-wall degradationMonosaccharide metabolismEnzyme supplementationFowl
Type
Effects of Complex Carbohydrates on Nutrient Absortion
Information
British Journal of Nutrition , Volume 67 , Issue 1 , January 1992 , pp. 103 - 114
Copyright
Copyright © The Nutrition Society 1992

References

Bogner, P. H. (1961) Alimentary absorption of reducing sugars by embryos and young chicks. Proceedings of the Society for Experimental Biology and Medicine 107, 263–275.CrossRefGoogle Scholar
Bogner, P. H. & Haines, I. A. (1964) Functional development of active sugar transport in the chick intestine. American Journal of Physiology 207, 37–41.CrossRefGoogle ScholarPubMed
Bolton, W. & Blair, R. (1974) Poultry Nutrition. London: H.M. Stationery Office.Google Scholar
Corley, R. C. (1929) Pentose metabolism. III. A comparison of the rates of disposal of d-arabinose and l-arabinose in the rabbit. Journal of Biological Chemistry 82, 269–272.CrossRefGoogle Scholar
Gasaway, W. C., White, R. G. & Holleman, D. F. (1976) Digestion of dry matter and absorption of water in the intestine and cecum of rock ptarmigan. Condor 78, 77–84.CrossRefGoogle Scholar
Jeffay, H. & Alvarez, J. (1961) Liquid scintillation counting of carbon–14. Use of ethanolamine–ethylene glycol monomethyl ether–toluene. Analytical Chemistry 33, 612–615.CrossRefGoogle Scholar
Kohn, P., Dawes, E. D. & Duke, J. W. (1965) Absorption of carbohydrates from the intestine of the rat. Biochimica et Biophysica Acta 107, 358–362.CrossRefGoogle ScholarPubMed
Longstaff, M. A., Knox, A. & McNab, J. M. (1988) Digestibility of pentose sugars and uronic acids and their effect on chick weight gain and caecal size. British Poultry Science 29, 379–393.CrossRefGoogle ScholarPubMed
Medway, W. & Kare, M. R. (1959) Blood and plasma volume, hematocrit, blood specific gravity and serum protein electrophoresis of the chicken. Poultry Science 38, 624–631.CrossRefGoogle Scholar
Savory, C. J. (1992) Enzyme supplementation, degradation and metabolism of three U–14C–labelled cell-wall substrates in the fowl. British Journal of Nutrition 67, 91–102.CrossRefGoogle Scholar
Savory, C. J. & Gentle, M. J. (1976) Changes in food intake and gut size in Japanese quail in response to manipulation of dietary fibre content. British Poultry Science 17, 571–580.CrossRefGoogle ScholarPubMed
Savory, C. J. & Knox, A. I. (1991) Chemical composition of caecal contents in the fowl in relation to dietary fibre level and time of day. Comparative Biochemistry and Physiology 100A, 739–743.CrossRefGoogle Scholar
Savory, C. J. & Mitchell, M. A. (1991) Absorption of hexose and pentose sugars in vivo in perfused intestinal segments in the fowl. Comparative Biochemistry and Physiology 100A, 969–974.CrossRefGoogle Scholar
Savory, C. J., Wood–Gush, D. G. M. & Duncan, I. J. H. (1978) Feeding behaviour in a population of domestic fowls in the wild. Applied Animal Ethology 4, 13–27.CrossRefGoogle Scholar
Wagh, P. V. & Waibel, P. E. (1966) Metabolizability and nutritional implications of l–arabinose and d-xylose for chicks. Journal of Nutrition 90, 207–211.CrossRefGoogle Scholar
Wagh, P. V. & Waibel, P. E. (1967a) Metabolism of l-arabinose and d-xylose by chicks. Journal of Nutrition 92, 491–496.CrossRefGoogle ScholarPubMed
Wagh, P. V. & Waibel, P. E. (1967b) Alimentary absorption of l-arabinose and d-xylose in chicks. Proceedings of the Society for Experimental Biology and Medicine 124, 421–424.CrossRefGoogle Scholar
Wood, F. C. & Cahill, G. F. (1963) Mannose utilization in man. Journal of Clinical Investigation 42, 1300–1312.CrossRefGoogle ScholarPubMed