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Metabolism of parenterally administered fat emulsions in the rat: studies of fatty acid oxidation with 1-13C- and 8-13C-labelled triolein

Published online by Cambridge University Press:  09 March 2007

Wolfgang Bäurle ,
Herbert Brösicke ,
Dwight E. Matthews ,
Karin Pogan  and
Peter Fürst
Wolfgang Bäurle
Affiliation:
Institute for Biological Chemistry and Nutrition, University of Hohenheim, Garbenstrasse 30, 70593 Stuttgart, Germany
Herbert Brösicke
Affiliation:
GCMS-Laboratory, Children's Hospital of the Free University of Berlin, Heubnerweg 16, 14059 Berlin, Germany
Dwight E. Matthews
Affiliation:
Department of Medicine, The University of Vermont College of Medicine, Given Building B217, Endo, Burlington, VT 05405, USA
Karin Pogan
Affiliation:
Institute for Biological Chemistry and Nutrition, University of Hohenheim, Garbenstrasse 30, 70593 Stuttgart, Germany
Peter Fürst*
Affiliation:
Institute for Biological Chemistry and Nutrition, University of Hohenheim, Garbenstrasse 30, 70593 Stuttgart, Germany
*
*Corresponding author:Prof. Peter Fürst, fax +49 711 4592283, email b-c-nutr@uni-hohenheim.de
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Abstract

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To reassess the hypothesis that fatty acid catabolism occurs to completion via β-oxidation, male Sprague–Dawley rats receiving continuous total parenteral nutrition (TPN) including 43% energy as fat were infused with [1-13C]- or [8-13C]triolein. Expired CO2 was collected continuously for 4 h and its 13C: 12C ratio determined by isotope–ratio mass spectrometry. Bicarbonate retention was also assessed over 4 h by infusion of NaH14CO3 and measurement of the expired 14CO2. A possible loss of label from [8-13C]oleic acid from the citric acid cycle via labelled acetyl-CoA without oxidation to CO2 was assessed by infusing further animals with acetate labelled with 14C either at C atoms 1 or 2 and determination of its conversion to expired 14CO2. At isotopic steady state, 63.2 (SE 1.6)% (n 8) of the infused [1-14C]acetate and 46.0 (SE 1.2)% (n 8) of [2-14C]acetate was recovered as expired 14CO2. After correction for bicarbonate retention and non-oxidative isotope loss, 37.3 (SE 1.2)% (n 20) of the [1-13C]triolein was found to have been oxidized, whereas 32.6 (SE 1.0)% (n 20) of the [8-13C]triolein was oxidized (P ≤ 0.01). The lower oxidation of the C atom at position 8 of oleic acid than that at position 1 indicates incomplete oxidative breakdown of the fatty acid after entering β-oxidation.

Keywords

13C-labelled trioleinβ-oxidationTotal parenteral nutrition
Type
General Nutrition
Information
British Journal of Nutrition , Volume 79 , Issue 4 , April 1998 , pp. 381 - 387
Copyright
Copyright © The Nutrition Society 1998

References

Adolph, M, Eckart, J, Metges, C, Nesser, G & Wolfram, G (1989) Oxidation of long and medium chain triglycerides during total parenteral nutrition of severely injured patients. In Nutrition in Clinical Practice: Proceedings of the 10th Congress ESPEN, Leipzig 1988, pp. 100–110 [Dietze,W Hartig, G W Hartig, G, Weiner, R, Fiirst, P, editors]. Basel: Karger.Google Scholar
Adolph, M, Eckart, J, Metges, C, Neeser, G & Wolfram, G (1991 a) Oxidation rates of (1- 13C) vs. (8-13C)-labeled triolein during TPN of septic ventilated patients. Journal of Parenteral and Enteral Nutrition 15, Suppl., 26S.Google Scholar
Adolph, M, Eckart, J, Metges, C, Neeser, G & Wolfram, G (1991 b) Recovery of (1-13C) vs. (8-13C)-labeled triolein during TPN of septic ventilated patients. Clinical Nutrition 10, Suppl., 16.Google Scholar
Allsop, JR, Wolfe, RR & Burke, JF (1978) Tracer priming the bicarbonate pool. Journal of Applied Physiology 45, 137–139.CrossRefGoogle ScholarPubMed
Armstrong, DT, Steele, R, Altszuler, N, Dunn, A, Bishop, JS & De Bodo, RC (1961) Regulation of plasma free fatty acid turnover. American Journal of Physiology 201, 9–15.Google Scholar
Bender, R, Haller, M & Fürst, P (1985) The influence of prior nutritional status on the oxidation rate of 14C-labelled MCT or LCT. Clinical Nutrition 4, Suppl., 63.Google Scholar
Brooks, GA & Donovan, CM (1983) Effect of endurance training on glucose kinetics during exercise. American Journal of Physiology 244, E505–E512.Google ScholarPubMed
Brösicke, H, Paust, H, Park, W, Knoblach, G & Helge, H (1985) Dependence of fat utilization on concomitant carbohydrate administration in parenterally fed low birth weight infants. Clinical Nutrition 4, Suppl., 56.Google Scholar
Chen, WJ (1984) Utilization of exogenous fat emulsion (Intralipid) in septic rats. Journal of Parenteral and Enteral Nutrition 8, 14–17.CrossRefGoogle ScholarPubMed
Eckart, J, Tempel, G, Kaul, A, Witzke, G, Schürbrand, P & Schaaf, H (1973) Metabolism of radioactive-labeled fat emulsions in the postoperative and posttraumatic period. American Journal of Clinical Nutrition 26, 578–582.Google Scholar
Geyer, RP, Chipman, J & Stare, FJ (1948) Oxidation in vivo of emulsified radioactive trilaurin administered intravenously. Journal of Biological Chemistry 176, 1469–1470.Google Scholar
Gould, RG, Sinex, FM, Rosenberg, IN, Solomon, AK & Hastings, AB (1949) Journal of Biological Chemistry 177, 295–301.Google Scholar
Greenberg, DM & Winnick, T (1949) The transformation in the rat of carboxyl-labeled acetate, methyl-labeled acetate, and labeled bicarbonate into amino acids. Archives of Biochemistry 21, 166–177.Google ScholarPubMed
Irving, CS, Lifschitz, CH, Wong, WW, Boutton, TW, Nichols, BL & Klein, PD (1985) [13C]Bicarbonate kinetics in humans: intra- vs. interindividual variations. Pediatric Research 19, 358–363.Google Scholar
Johnson, RJ, Young, SK, Cotter, R, Lin, L & Rowe, B (1990) Medium-chain-triglyceride lipid emulsion: metabolism and tissue distribution. American Journal of Clinical Nutrition 52, 502–508.CrossRefGoogle ScholarPubMed
Lerner, SR, Chaikoff, IL, Entenman, C & Dauben, WG (1949) The fate of C-14-labeled palmitic acid administered intravenously as a tripalmitin emulsion. Proceedings of the Society for Experimental Biology and Medicine 70, 384–387.Google Scholar
Metges, CC, Kempe, K & Wolfram, G (1992) Relative 13CO2 recovery rates of orally administered [1-13C] and [8-13C]triolein and 13C enrichments of fatty acids in selected serum lipids in humans. Clinical Nutrition 11, Suppl., 20.CrossRefGoogle Scholar
Moldawer, LL, Kawamura, I, Bistrian, BR & Blackburn, GL (1983) The contribution of phenylalanine to tyrosine metabolism in vivo: studies in the post-absorptive and phenylalanine-loaded rat. Biochemical Journal 210, 811–817.Google Scholar
Morris, B & Simpson-Morgan, MW (1963) The excretion of 14CO2 during the continuous intravenous infusion of NaH14CO3 in unanaesthetized rats. Journal of Physiology 169, 713–728.CrossRefGoogle ScholarPubMed
Nordenström, J, Carpentier, YA, Askanazi, J, Robin, AP, Elwyn, DH, Hensle, TW & Kinney, JM (1982) Metabolic utilization of intravenous fat emulsion during total parenteral nutrition. Annals of Surgery 196, 221–231.Google Scholar
Park, W, Paust, H, Brösicke, H, Knoblach, G & Helge, H (1986) Impaired fat utilization in parenterally fed low-birth-weight infants suffering from sepsis. Journal of Parenteral and Enteral Nutrition 10, 627–630.CrossRefGoogle ScholarPubMed
Paust, H, Park, W, Rating, D & Helge, H (1984) Measurement of fatty acid oxidation in premature newborn infants with the 13C-triolein breath test. Clinical Nutrition 3, 89–92.Google Scholar
Paust, H, Park, W, Knoblach, G, Keles, T & Scigalla, P (1989) Measurement of substrate utilization by 13C tracer infusion technique. In Nutrition in Clinical Practice: Proceedings of the 10th Congress ESPEN, Leipzig 1988, pp. 111–128 [Dietze,W Hartig, G W Hartig, G, Weiner, R, Fürst, P, editors]. Basel: Karger.Google Scholar
Schoeller, DA, Brown, C, Nakamura, K, Nagakawa, A, Mazzeo, RS, Brooks, GA & Budinger, TF (1984) Influence of metabolic fuel on the 13C/12C ratio of breath CO2. Biomedical Mass Spectro-metry 11, 557–561.CrossRefGoogle ScholarPubMed
Schoeller, DA, Klein, PD, Watkins, JB, Heim, T & MacLean, WC Jr (1980) 13C abundances of nutrients and the effect of variations in 13C isotopic abundance of test meals formulated for 13CO2 breath test. American Journal of Clinical Nutrition 33, 2375–2385.Google Scholar
Scrimgeour, CM & Rennie, MJ (1988) Automated measurement of the concentration and 13C enrichment of carbon dioxide in breath and blood samples using the Finnigan MAT breath gas analysis system. Biomedical and Environmental Mass Spectro-metry 15, 365–367.CrossRefGoogle ScholarPubMed
Shipley, RA, Baker, N, Incefy, GE & Clark, RE (1959) 14C Studies in carbohydrate metabolism. IV. Characteristics of bicarbonate pool system in the rat. American Journal of Physiology 197, 41–46.CrossRefGoogle Scholar
Silberman, H (1986) Total parenteral nutrition by peripheral vein: current status of fat emulsions. Nutrition International 2, 145–149.Google Scholar
Statistical Analysis Systems (1988) SAS/STAT™ User's Guide, Release 6.03 Edition. Cary, NC: SAS Institute Inc.Google Scholar
Sulkers, EJ, Lafeber, HN & Sauer, PJJ (1989) Quantitation of oxidation of medium-chain triglycerides in preterm infants. Pediatric Research 26, 294–297.CrossRefGoogle ScholarPubMed
Thompson, GN, Pacy, PJ, Ford, GC & Halliday, D (1989) Practical considerations in the use of stable isotope labelled compounds as tracers in clinical studies. Biomedical and Environmental Mass Spectrometry 18, 321–327.CrossRefGoogle ScholarPubMed
Vazquez, JA, Paul, HS & Adibi, SA (1986) Relation between plasma and tissue parameters of leucine metabolism in fed and starved rats. American Journal of Physiology 250, E615–E621.Google Scholar
Watkins, JB, Schoeller, DA, Klein, PD, Ott, DG, Newcomer, AD & Hofman, AF (1977) 13C-Trioctanoin: a nonradioactive breath test to detect fat malabsorption. Journal of Laboratory and Clinical Medicine 90, 422–430.Google Scholar
Weinman, EO, Chaikoff, IL, Dauben, WG, Gee, M & Entenman, C (1950) Relative rates of conversion of the various carbon atoms of palmitic acid to carbon dioxide by the intact rat. Journal of Biological Chemistry 184, 735–744.CrossRefGoogle ScholarPubMed
Weinman, EO, Chaikoff, IL, Stevens, BP & Dauben, WG (1951) Conversion of first and sixth carbons of stearic acid to carbon dioxide by rats. Journal of Biological Chemistry 191, 523–529.CrossRefGoogle ScholarPubMed
Weinmann, EO, Strisower, EH & Chaikoff, IL (1957). Conversion of fatty acids to carbohydrate: application of isotopes to this problem and role of the Krebs cycle as a synthetic pathway. Physiological Reviews 37, 252–272.CrossRefGoogle Scholar
Wolfe, RR (1984) Tracers in metabolic research: radioisotope and stable isotope. In Mass Spectrometry Methods, pp. 55–59. New York: Alan R. Liss, Inc.Google ScholarPubMed
Wolfe, RR & Jahoor, F (1990) Recovery of labeled CO2 during the infusion of C-1 v. C-2-labeled acetate: implications for tracer studies of substrate oxidation. American Journal of Clinical Nutrition 51, 248–252.CrossRefGoogle Scholar
Wolfram, G & Metges, C (1988) Fatty acid oxidation following enteral or parenteral application of 13C-labeled medium chain and long chain triglycerides. In Klinische Ernährung 34: Use of Stable Isotopes in Clinical Research and Practice, pp. 89–92 [Park,H Paust, W H Paust, W, Helge, H, Scigalla, P, editors]. München: W. Zuckschwerdt Verlag.Google Scholar
Yagi, M & Walser, M (1990) Estimation of whole body protein synthesis from oxidation of infused [1-14C]-leucine. American Journal of Physiology 258, E151–E157.Google Scholar
Yang, RD, Irving, CS, Wong, WW, Hoffer, JH, Young, VR & Klein, PD (1983) The effect of diet and meal ingestion on whole body 13C-bicarbonate kinetics in young men. Federation Proceedings 42, 825.Google Scholar