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Na+/glucose co-transporter abundance and activity in the small intestine of lambs: enhancement by abomasal infusion of casein

Published online by Cambridge University Press:  09 March 2007

Sameer J. Mabjeesh ,
Dafna Guy  and
David Sklan
Sameer J. Mabjeesh*
Affiliation:
Department of Animal Sciences, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Dafna Guy
Affiliation:
Department of Animal Sciences, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
David Sklan
Affiliation:
Department of Animal Sciences, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
*
*Corresponding Author: Dr Sameer J. Mabjeesh, fax +972 8 9489 867, email Mabjeesh@agri.huji.ac.il
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Abstract

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The purpose of the present study was to determine the effect of abomasal casein infusion on glucose uptake and abundance of the Na+/glucose co-transporter (SGLT1) 1 in the ovine small intestine. Lambs (body weight 35 (SEM 1·0) kg) were surgically fitted with abomasal infusion catheters and were fed diets containing equal portions of wheat hay and cracked maize. Lambs were infused with either 500 g water/d or with 500 g water containing 35 g casein/d. The infusion period lasted 10 d, after which lambs were killed, exsanguinated and eviscerated. Brush border membrane vesicles (BBMV) were prepared using mucosa from different small intestinal regions. Intake and total tract digestibility of nutrients were similar between treatments and averaged 1134, 1142 and 486 g/d and 67, 70, and 94 % for DM, organic matter and non-structural carbohydrates respectively. Crude protein (N×6·25) digestibility was 15 % greater in the casein-infused than control lambs. Glucose uptake to BBMV ranged from 101 to 337 pmol/mg protein per s along the small intestine and was greatest in the mid-section of the small intestine. In the mid-jejunum, glucose uptake was greater (P<0·07) in lambs infused with casein and averaged 120 pmol/mg protein per s compared with 68 pmol/mg protein per s in the control group. SGLT1 affinity was similar between treatments and averaged 104 μM in the different segments of the small intestine of lambs. However, lambs infused with casein exhibited similar values along the small intestine and affinity averaged 106 μm, while in the control group a greater affinity (85 μm) was measured in the mid-jejunum. SGLT1 protein abundance was correlated with glucose uptake in the BBMV in the casein-treated lambs, but not in the control group. These results suggest that glucose uptake along the small intestine of lambs is influenced by casein or its derivatives in the small intestine via SGLT1 affinity and activity at the brush border membrane, and that SGLT1 activity may be regulated by post-translational events affected by amino acids and peptides.

Keywords

Starch digestionGlucose transporterSheep
Type
Research Article
Information
British Journal of Nutrition , Volume 89 , Issue 5 , May 2003 , pp. 573 - 580
Copyright
Copyright © The Nutrition Society 2003

References

Allison, GG & Shirazi-Beechey, SP (1997) Measurements of ovine SGLT1 mRNA level by competitive RT-PCR. Biochemical Society Transactions 25, 418s.CrossRefGoogle ScholarPubMed
Arieli, A, Abramson, S, Mabjeesh, SJ, Zamwel, S & Bruckental, I (2000) Effect of site and source of energy supplementation on milk yield in dairy cows. Journal of Dairy Science 84 462–470.CrossRefGoogle Scholar
Association of Official Analytical Chemists (1990) Official Methods of Analysis, 15th ed., Arlington, VA: AOAC.Google Scholar
Barry, TN, Manley, TR, Redekopp, C, Davis, SR, Fairclough, RJ & Lapwood, KR (1982) Protein metabolism in growing lambs given fresh ryegrass (Lolium perenne) and clover (Trifolium repens) pasture ad libitum. 2. Endocrine changes, glucose production, and their relationship to protein deposition and the partition of absorbed nutrients. British Journal of Nutrition 47 319–329.CrossRefGoogle Scholar
Bauer, ML (1992) Nutritional regulation of small intestinal glucose absorption in ruminants. PhD Thesis, University of KentuckyGoogle Scholar
Bauer, ML, Harmon, DL, Bohnert, DW, Branco, AF & Huntington, GB (2001) Influence of α-linked glucose on sodium–glucose cotransport activity along the small intestine in cattle. Journal of Animal Science 79 1917–1924.CrossRefGoogle ScholarPubMed
Dyer, J, Hosie, KB & Shirazi-Beechey, SP (1997) Nutrient regulation of human intestinal glucose transporter (SGLT1) expression. Gut 41 56–59.CrossRefGoogle ScholarPubMed
Freeman, HJ & Quamme, GA (1986) Age related changes in sodium-dependent glucose transporter in rat small intestine. American Journal of Physiology 251 G208–G217.Google Scholar
Freeman, TC, Wood, IS, Sirinathsinghji, DJS, Beechey, RB, Dyer, J & Shirazi-Beechey, SP (1993) The expression of Na+/glucose cotransporter (SGLT1) gene in lamb small intestine during postnatal development. Biochimica et Biophysica Acta 1146 202–212.Google ScholarPubMed
Gal-Garber, O, Mabjeesh, SJ, Sklan, D & Uni, Z (2000) Sodium glucose transporter (SGLT-1) in the chicken small intestine: partial sequence of the gene expression and activity on starved and re-feeding. Journal of Nutrition 130 2174–2179.CrossRefGoogle Scholar
Gross, KL, Harmon, DL, Minton, JE & Avery, TB (1990) Effects of isoenergetic infusions of propionate and glucose on portal-drained visceral nutrient flux and concentrations of hormones in lambs maintained by total intragastric infusion. Journal of Animal Science 68 2566–2574.CrossRefGoogle ScholarPubMed
Harmon, DL (1993) Nutritional regulation of postruminal digestive enzymes in ruminants. Journal of Dairy Science 76 2102–2109.CrossRefGoogle ScholarPubMed
Huntington, GB (1997) Starch utilization by ruminants: from basics to the bunk. Journal of Animal Science 75 852–867.CrossRefGoogle Scholar
Huber, JT, Jacobson, NL, McGilliard, AD & Allen, RS (1961) Utilization of carbohydrates introduced directly into the omaso-abomasal area of the stomach of cattle of various ages. Journal of Dairy Science 44 321–330.CrossRefGoogle Scholar
Hussein, HS, Jordan, RM & Stern, MD (1991) Ruminal protein metabolism and intestinal amino acid utilization as affected by dietary protein and carbohydrate sources in sheep. Journal of Animal Science 69 2134–2346.CrossRefGoogle ScholarPubMed
Kellet, LG & Helliwell, PA (2000) The diffusive component of intestinal glucose absorption is mediated by the glucose-induced recruitment of GLUT2 to the brush border membrane. Biochemistry Journal 350 155–162.CrossRefGoogle Scholar
Knowlton, KF, Dawson, TE, Glenn, BP, Huntington, GB & Erdman, RA (1998) Glucose metabolism and milk yield of cows infused abomasally or ruminally with starch. Journal of Dairy Science 81 3248–3258.CrossRefGoogle ScholarPubMed
Krehbeil, CR, Britton, RA, Harmon, DL, Peters, JP, Stock, RA & Grotjan, HE (1996) Effects of varying levels of duodenal or midjejunal glucose and 2-deoxyglucose infusion on small intestinal disappearance and net portal glucose flux in steers. Journal of Animal Science 74 693–700.CrossRefGoogle Scholar
Kreikemeier, KK, Harmon, DL, Peters, JP, Gross, KL, Armendariz, CK & Krehbiel, CR (1990) Influence of dietary forage and feed intake on carbohydrase activities and small intestinal morphology of calves. Journal of Animal Science 68 2916–2929.CrossRefGoogle ScholarPubMed
Kuhn, Fuchs M, Beck, FX, Martin, S, Jahne, J, Klempnauer, J, Kaever, V, Rechkemmer, G & Forssmann, WG (1997) Endothelin-1 potently stimulates chloride secretion and inhibits Na(+)-glucose absorption in human intestine in vitro. Journal of Physiology London 499 391–402.CrossRefGoogle ScholarPubMed
Laemmli, UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 680–685.CrossRefGoogle ScholarPubMed
Larson, HJ, Stoddart, GE, Jacboson, NL & Allen, RS (1956) Digestion and absorption of various carbohydrates posterior to the rumino-reticular area of the young bovine. Journal of Animal Science 15 473–484.CrossRefGoogle Scholar
Le Drean, G, Huerou-Luron, LeI, Philouze-Rome, V, Toullec, R & Guilloteau, P (1995) Response of the calf pancreas to differently processed soya bean and pea diets. Annals of Nutrition and Metabolism 39 164–176.Google ScholarPubMed
Lescale-Matys, L, Dyer, J, Scott, D, Freeman, TC, Wright, EM & Shirazi-Beechey, SP (1993) Regulation of the ovine intestinal Na+/glucose co-transporter (SGLT1) is dissociated from mRNA Abundance. Biochemical Journal 291 435–440.CrossRefGoogle ScholarPubMed
National Research Council (1989) Nutrient Requirements of Dairy Cattle, 6th revised ed, Washington, DC: National Academy of Science.Google Scholar
Ørskov, ER, Fraser, C, Mason, VC & Mann, SO (1970) Influence of starch digestion in the large intestine of sheep on caecal fermentation, caecal microflora and faecal nitrogen excretion. British Journal of Nutrition 24 671–682.CrossRefGoogle ScholarPubMed
Owens, FN, Zinn, RA & Kim, YK (1986) Limits to starch digestion in the ruminant small intestine. Journal of Animal Science 63 1634–1648.CrossRefGoogle ScholarPubMed
Panayotova-Heiermann, M, Loo, DDF, Kong, CT, Lever, J & Wright, EM (1996) Sugar binding to Na+/glucose cotransporters is determined by the carboxy-terminal half of the protein. Journal of Biochemical Chemistry 271 10029–10043.Google Scholar
Reinhardt, J, Veyhl, M, Wagner, K, Gambaryan, S, Dekel, C, Akhoundova, A, Korn, T & Koepsell, H (1999) Cloning and characterization of the transport modifier RS1 from rabbit which was previously assumed to be specific for Na+-d-glucose cotransport. Biochimica et Biophysica Acta 1417 131–143.CrossRefGoogle ScholarPubMed
Richards, CJ, Swanson, KC & Harmon, DL (1998) Effect of postruminal protein infusion on pancreatic exocrine secretion in beef steers. Journal of Animal Science 76, Suppl. 1, 312, AbstrGoogle Scholar
Shabi, Z, Bruckental, I, Zamwell, S, Tagari, H & Arieli, A (1999) Effects of extrusion of grain and feeing frequency on rumen fermentation, nutrients digestibility, and milk yield and composition in dairy cows. Journal of Dairy Science 82 1252–1260.CrossRefGoogle Scholar
Shimizu, K, Shiratori, K, Watanabe, S, Takeuchi, T, Chang, TM & Chey, WY (1994) Effects of protein derivatives on pancreatic secretion and release of secretin and CCK in rats. American Journal of Physiology 267 G508–G514.Google ScholarPubMed
Shirazi-Beechey, SP, Dyer, J, Allison, G & Wood, IS (1996) Nutrient regulation of intestinal sugar-transporter expression. Biochemical Society Transactions 24 389–392.CrossRefGoogle ScholarPubMed
Shirazi-Beechey, SP, Gribble, SM, Wood, IS, Tarpey, PS, Beechey, RB, Dyer, J, Scott, D & Barker, PJ (1994) Dietary regulation of the intestinal sodium-dependent glucose cotransporter (SGLT1). Biochemical Society Transactions 223 655–658.CrossRefGoogle Scholar
Shirazi-Beechey, SP, Hirayama, BA, Wang, Y, Scott, D, Smith, MW & Wright, EM (1991) Ontogenic development of lamb intestinal sodium–glucose co-transporter is regulated by diet. Journal of Physiology 437 699–708.CrossRefGoogle ScholarPubMed
Sklan, D, Arieli, A, Chalupa, W & Kronfeld, DS (1985) Digestion and absorption of lipids and bile acids in sheep fed stearic acid, oleic acid, or tristearin. Journal of Dairy Science 68 1667–1675.CrossRefGoogle ScholarPubMed
Sklan, D & Halevy, O (1985) Digestion and absorption of protein along ovine gastrointestinal tract. Journal of Dairy Science 68 1676–1981.CrossRefGoogle ScholarPubMed
Sklan, D & Hurwitz, S (1985) Movement and absorption of major minerals and water in ovine gastrointestinal tract. Journal of Dairy Science 68 1659–1666.CrossRefGoogle ScholarPubMed
Smith, D (1981) Removing and Analyzing Carbohydrates from Plants Tissue. Wisconsin Agriculture Experimental Station Reports no. R2107. Madison, WI: Wisconsin Agriculture.Google Scholar
Swanson, KC, Matthews, JC, Matthews, AD, Howell, JA, Richards, CJ & Harmon, DL (2000) Dietary carbohydrate source and energy intake influence the expression of pancreatic alpha-amylase in lambs. Journal of Nutrition 130 2157–2165.CrossRefGoogle ScholarPubMed
Swanson, KC, Richards, CJ & Harmon, DL (1998) Influence of abomasal infusion of glucose or starch hydrolysate on pancreatic exocrine secretion in beef steers. Journal of Animal Science 76, Suppl. 1, 313, AbstrGoogle Scholar
Taniguchi, K, Huntington, GB & Glenn, BP (1995) Net nutrient flux by visceral tissues of beef steers given abomasal and ruminal infusions of casein and starch. Journal of Animal Science 73 236–249.CrossRefGoogle ScholarPubMed
Uden, P, Colucci, PE & Van Soest, PJ (1980) Investigation of chromium, cerium and cobalt as markers in digesta. Rate of passage studies. Journal of Science, Food and Agriculture 31 625–632.CrossRefGoogle ScholarPubMed
Van Soest, PJ, Robertson, JB & Lewis, BA (1991) Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74 3583–3597.CrossRefGoogle ScholarPubMed
Vayro, S & Silverman, M (1999) PKC regulates turnover rate of rabbit intestinal Na+-glucose transporter expressed in COS-7 cells. American Journal of Physiology 276 C1053–C1060.CrossRefGoogle ScholarPubMed
Veyhl, M, Spangenberg, J, Puschel, B, Poppe, R, Dekel, C, Fritzsch, G, Haase, W & Koepsell, H (1993) Cloning of a membrane-associated protein which modifies activity and properties of the Na(+)-d-glucose cotransporter. Journal of Biochemical Chemistry 268 25041–25053.Google ScholarPubMed
Walker, JA & Harmon, DL (1995) Influence of ruminal or abomasal starch hydrolysate infusion on pancreatic exocrine secretion and blood glucose and insulin concentrations in steers. Journal of Animal Science 73 3766–3774.CrossRefGoogle ScholarPubMed
Zhao, FQ, Okine, EK, Cheesman, CI, Shirazi-Beechey, SP & Kennelly, JK (1998) Glucose transporter gene expression in lactating bovine gastrointestinal tract. Journal of Animal Science 76 2921–2929.CrossRefGoogle ScholarPubMed