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Olive oil- and fish oil-enriched diets modify plasma lipids and susceptibility of LDL to oxidative modification in free-living male patients with peripheral vascular disease: the Spanish Nutrition Study

Published online by Cambridge University Press:  09 March 2007

Carmen Ramírez-Tortosa ,
Jose M. López-Pedrosa ,
Antonio Suarez ,
Eduardo Ros ,
José Mataix  and
Angel Gil
Carmen Ramírez-Tortosa*
Affiliation:
Institute of Nutrition and Food Technology, University of Granada, Spain Department of Biochemistry and Molecular Biology, University of Granada, Campus de Cartuja, 18071 Granada, Spain
Jose M. López-Pedrosa
Affiliation:
Department of Biochemistry and Molecular Biology, University of Granada, Campus de Cartuja, 18071 Granada, Spain
Antonio Suarez
Affiliation:
Department of Biochemistry and Molecular Biology, University of Granada, Campus de Cartuja, 18071 Granada, Spain
Eduardo Ros
Affiliation:
Department of Internal Medicine, Vascular Surgery Service, Clinic Hospital, University of Granada, Spain
José Mataix
Affiliation:
Institute of Nutrition and Food Technology, University of Granada, Spain
Angel Gil
Affiliation:
Institute of Nutrition and Food Technology, University of Granada, Spain Department of Biochemistry and Molecular Biology, University of Granada, Campus de Cartuja, 18071 Granada, Spain
*
*Corresponding author: Dr M. del Carmen Ramírez-Tortosa, fax +34 958 281164, email mramirez@goliat.ugr.es
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Abstract

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The present study describes a clinical trial in which Spanish patients suffering from peripheral vascular disease (Fontaine stage II) were given specific lipid supplements. Designed as a longitudinal intervention study, patients were provided with olive oil for 3 months, followed by a 3 month wash-out period, then supplemented with a combination of fish oil and olive oil for the final 3 months. Changes in plasma and lipoprotein fatty acid composition and susceptibility of LDL to in vitro oxidation were examined. Furthermore, lipid-supplement-induced changes in LDL properties were measured as relative electrophoretic mobility and macrophage uptake. In addition, thirteen patients not provided with olive oil and fish oil were included as a control group and twenty healthy age-matched individuals were used as a reference group. A complete clinical study and a nutritional survey concerning food habits and lifestyle were performed every 3 months. Yao indices and claudicometry did not change significantly with dietary intervention although changes in plasma lipid composition suggested an improvement in the condition of the patients. The intake of the fish-oil supplement resulted in significantly increased plasma levels of eicosapentaenoic acid (20:5n−3) and docosahexaenoic acid (22:6n−3) in comparison with baseline concentrations, olive-oil and control groups. Fish-oil consumption significantly decreased plasma triacylglycerol levels compared with the olive-oil period, control and reference groups. The susceptibility of LDL to Cu-mediated oxidation was lower in the patients consuming olive oil and the fish-oil supplement than in the control group, and the uptake of LDL by macrophages was significantly lower in the group supplemented with fish oil. In conclusion, consumption of olive oil together with a dietary supplement of fish oil may be useful in the nutritional management of patients suffering from peripheral vascular disease in terms of increasing plasma n−3 long-chain polyunsaturated fatty acids and decreasing susceptibility of LDL to oxidation.

Keywords

Fish oilOlive oilPeripheral vascular diseaseLDL oxidation
Type
Research Article
Information
British Journal of Nutrition , Volume 82 , Issue 1 , July 1999 , pp. 31 - 39
Copyright
Copyright © The Nutrition Society 1999

References

Aguilera, F, Lupianez, L, Magana, D, Planells, E, Mataix, FJ & Llopis, J (1996) Lipid status in a population of Spanish schoolchildren. European Journal of Epidemiology 12, 135–140.Google Scholar
Berry, EM, Eisenberg, S, Haratz, D, Friedlander, Y, Norman, Y, Kaufmann, NA & Yechezkielstein, S (1991) Effects of diets rich in monounsaturated fatty acid on plasma lipoproteins. The Jerusalem Nutrition Study: high monounsaturated fatty acids vs. polyunsaturated fatty acids. American Journal of Clinical Nutrition 53, 899–907.CrossRefGoogle Scholar
Bollinger, A (1982) Angiologia Funcional (Functional Angiology), pp. 61–78. Barcelona: Tonay S.A.Google Scholar
Bonanome, A, Biasia, F, De Luca, M, Munaretto, G, Biffanti, S, Pradella, M & Pagnan, A (1996) N-3 Fatty acids do not enhance LDL susceptibility to oxidation in hypertriacylglycerolemic hemodialyzed subjects. American Journal of Clinical Nutrition 63, 261–266.Google Scholar
Bonanome, A, Pagnan, A, Biffani, S, Opportuno, A, Sogato, F, Dorella, M, Maiorino, M & Ursini, F (1992) Effect of dietary monounsaturated and polyunsaturated fatty acids on the susceptibility of plasma low density lipoproteins to oxidative modification. Arteriosclerosis and Thrombosis 12, 529–533.Google Scholar
Bradford, MM (1979) A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Annals of Biochemistry 72, 248–254.Google Scholar
Buege, JA & Aust, SD (1978) Microsomal lipid peroxidation. Methods in Enzymology 52, 302–310.Google Scholar
Chung, BH, Wilkinson, T, Geer, JC & Segrest, JP (1981) Preparative and quantitative isolation of plasma lipoprotein: rapid, single discontinuous density gradient ultracentrifugation in a vertical rotor. Journal of Lipid Research 21, 284–291.Google Scholar
Eritsland, J, Arnesen, H, Seljeflot, I & Hostmark, AT (1995) Long-term metabolic effects of n−3 polyunsaturated fatty acids in patients with coronary artery disease. American Journal of Clinical Nutrition 61, 831–836.Google Scholar
Flaten, H, Hostmark, AT, Kierulf, P, Lystad, E, Trygg, K, Bjerkedal, T & Osland, A (1990) Fish-oil concentrate: effects on variables related to cardiovascular disease. American Journal of Clinical Nutrition 52, 300–306.CrossRefGoogle ScholarPubMed
Frei, B & Gaziano, JM (1993) Content of antioxidants, preformed lipid hydroperoxides, and cholesterol as predictors of the susceptibility of human LDL to metal ion-dependent and ion-independent oxidation. Journal of Lipid Research 34, 2135–2145.Google Scholar
Friedewald, WT, Levy, RI & Fredrickson, DS (1972) Estimation of the concentration of low density lipoprotein cholesterol in plasma without use of the preparative ultracentrifuge. Clinical Chemistry 18, 499–502.CrossRefGoogle ScholarPubMed
Gartside, PS & Glueck, CJ (1995) The important role of modifiable dietary and behavior characteristics in the causation and prevention of coronary heart disease hospitalisation and mortality: the prospective NHANES I follow-up study. Journal of the American College of Nutrition 14, 71–79.Google Scholar
Green, P, Fusch, J, Shoenfeld, N, Leibovici, L, Lurie, Y, Bigel, Y, Rotenberg, Z, Mamet, R & Budowski, P (1990) Effects of fish oil ingestion on cardiovascular risk factors in hyperlipidemic subjects in Israel: a randomized, double-blind crossover study. American Journal of Clinical Nutrition 52, 1118–1124.Google Scholar
Grundy, SM & Denke, SM (1990) Dietary influences on serum lipids and lipoproteins. Journal of Lipid Research 31, 1149–1172.Google Scholar
Gurr, M (1992) Dietary lipid and coronary heart disease. Progress in Lipid Research 31, 195–243.CrossRefGoogle ScholarPubMed
Hirai, A, Terano, T, Hamzaki, T, Sajiki, J, Londo, S, Ozawa, A, Fukita, T, Miyamoto, T, Tamura, Y & Kumagai, A (1982) The effect of oral administration of fish oil concentrate on the release and the metabolism of (14C) arachidonic acid and (14C) eicosapentaenoic acid by human platelets. Thrombosis Research 28, 285–298.CrossRefGoogle Scholar
Jialal, I & Grundy, SM (1991) Preservation of the endogenous antioxidants in low density lipoprotein by ascorbate but not probucol during oxidative modification. Journal of Clinical Investigation 87, 597–601.Google Scholar
Keys, A, Menotti, A & Karvonene, MJ (1986) The diet and 15-years death rate in the Seven Country Study. American Journal of Epidemiology 124, 903–915.Google Scholar
Lepage, G & Roy, C (1986) Direct transesterification of all classes of lipids in a one-step reaction. Journal of Lipid Research 27, 114–120.Google Scholar
Mancini, M, Parfitt, VJ & Rubba, P (1995) Antioxidants in the Mediterranean diet. Canadian Journal of Cardiology 11, 105–109.Google ScholarPubMed
Mataix, J, Mañas, M, Llopis, J & Martinez-Victoria, E (1994) Alimentacion y Salud. Programa Informatico de Nutrition (Food and Health. Nutrition Information Program). Valencia,: Asde Alimentacion S.A.Google Scholar
Mataix, J, Mañas, M, Llopis, J & Martinez-Victoria, E (1994) Tabla de Composicion de Alimentos Españoles (Spanish Food Composition Tables). Granada, Spain: Universidad de Granada.Google Scholar
Mattson, FH & Grundy, SM (1985) Comparison of effects of dietary saturated, monounsaturated, and polyunsaturated fatty acids on plasma lipids and lipoproteins in man. Journal of Lipid Research 26, 1940–2002.Google Scholar
Mori, TA, Vandongen, R, Mahanian, F & Douglas, A (1992) Plasma lipid levels and platelet and neutrophil function in patients with vascular disease following fish oil and olive oil supplementation. Metabolism 41, 1059–1067.Google Scholar
Noble, PR (1968) Electrophoretic separation of plasma lipoproteins in agarose gel. Journal of Lipid Research 9, 693–700.Google Scholar
Norman, I (1993) Action of carotenoids in biological systems. Annual Review of Nutrition 13, 561–570.Google Scholar
Radack, KL, Deck, CC & Huster, GA (1990) n−3 Fatty acid effects on lipids, lipoproteins, and apolipoproteins at very low doses: results of a randomized controlled trial in hypertriglyceridemic subjects. American Journal of Clinical Nutrition 51, 599–605.Google Scholar
Reaven, P, Parthasarathy, S, Grasse, BJ, Miller, E, Almazan, F, Mattson, FH, Khoo, JC, Steinberg, D & Witztum, JL (1991) Feasibility of using an oleate rich diet to reduce the susceptibility of low density lipoprotein to oxidative modification in humans. American Journal of Clinical Nutrition 54, 791–806.Google Scholar
Sacks, FM, Stone, PM, Gibson, CM, Silverman, DI, Rosner, B & Pasternak, R (1995) Controlled trial of fish oil for regression of human coronary atherosclerosis. Journal of the American College of Cardiology 25, 1492–1498.Google Scholar
Schmidt, EB, Lervang, HM, Barming, K, Madsen, P & Dyerberg, J (1992) Long-term supplementation with n−3 fatty acids, I: Effect on blood lipids, homeostasis and blood pressure. Scandinavian Journal of Clinical and Laboratory Investigation 52, 229–236.Google Scholar
Sola, R, La Ville, AE, Richard, JL, Motta, C, Bargallo, MT, Girona, J, Masana, I & Jacotot, B (1997) Oleic acid rich diet protects against the oxidative modification of high density lipoprotein. Free Radical Biology and Medicine 22, 1037–1045.Google Scholar
Steinberg, D, Parthasarathy, S, Carew, TE, Khoo, J & Witztum, JL (1989) Beyond cholesterol: modifications of low density lipoprotein that increase its atherogenicity. New England Journal of Medicine 320, 915–924.Google Scholar
Suzukawa, M, Abbey, M, Clifton, P & Nestel, PJ (1996) Enhanced capacity of n−3 fatty acid enriched macrophages to oxidize low density lipoprotein: mechanisms and effects of antioxidant vitamins. Atherosclerosis 124, 157–169.Google Scholar
Suzukawa, M, Abbey, M, Howe, PR & Nestel, PJ (1995 a) Effects of fish oil fatty acids on low density lipoprotein size, oxidizability and uptake by macrophages. Journal of Lipid Research 36, 473–484.Google Scholar
Suzukawa, M, Ishikaea, T, Yoshida, H & Nakamura, H (1995 b) Effect of in vivo supplementation with low dose vitamin E on susceptibility of low density lipoprotein and high density lipoprotein to oxidative modification. Journal of the American College of Nutrition 14, 46–52.Google Scholar
Thurnham, DI, Smith, E & Flora, PS (1988) Concurrent liquid chromatographic assay of retinol, α-tocopherol, β-carotene, α- carotene, lycopene and β-cryptoxanthin in plasma, with tocopherol acetate as internal standard. Clinical Chemistry 34, 377–381.Google Scholar
Vega, G, Groszek, E, Wolf, R & Grundy, SM (1982) Influence of polyunsaturated fats on composition of plasma lipoproteins and apolipoproteins. Journal of Lipid Research 23, 811–822.CrossRefGoogle ScholarPubMed
Visioli, F, Bellomo, G, Montedoro, GF & Galli, C (1995) Low density lipoprotein oxidation is inhibited in vitro by olive oil constituents. Atherosclerosis 117, 25–32.Google Scholar
Whitman, S, Fish, J, Rand, M & Roger, K (1994) n−3 Fatty acid incorporation into LDL particles renders them more susceptible to oxidation in vitro but not necessarily more atherogenic in vivo. Atherosclerosis and Thrombosis 14, 1170–1176.Google Scholar
Witztum, JL & Steinberg, D (1991) Role of oxidized low density lipoprotein in atherogenesis. Journal of Clinical Investigation 88, 1785–1792.CrossRefGoogle ScholarPubMed
Zouhair, FS & Edna, CY (1993) Rapid fluorometric assay of LDL receptor activity by DiI-labelled LDL. Journal of Lipid Research 34, 325–330.Google Scholar