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Oral ingestion of lactic-acid bacteria by rats increases lymphocyte proliferation and interferon-γ production

Published online by Cambridge University Press:  09 March 2007

Najat Aattouri ,
Mohammed Bouras ,
Daniel Tome ,
Ascension Marcos  and
Daniel Lemonnier
Najat Aattouri*
Affiliation:
INRA, Laboratoire de Nutrition Humaine et de Physiologie Intestinale, Institut National Agronomique Paris-Grignon, F-75231 Paris, France Groupe de Recherche en Pédiatrie de Normandie, CHU Charles Nicolle, F-76031 Rouen, France
Mohammed Bouras
Affiliation:
INRA, Laboratoire de Nutrition Humaine et de Physiologie Intestinale, Institut National Agronomique Paris-Grignon, F-75231 Paris, France
Daniel Tome
Affiliation:
INRA, Laboratoire de Nutrition Humaine et de Physiologie Intestinale, Institut National Agronomique Paris-Grignon, F-75231 Paris, France
Ascension Marcos
Affiliation:
Instituto de Nutrition y Bromatologia, Facultad de Pharmacia, Ciudad Universitaria, 28040 Madrid, Spain
Daniel Lemonnier
Affiliation:
Groupe de Recherche en Pédiatrie de Normandie, CHU Charles Nicolle, F-76031 Rouen, France
*
*Corresponding author: Dr Najat Aattouri, present address STELLA, FSSA Pavillon Paul-comtois, local 1316, Université Laval, G1K 7P4, Québec, Canada, fax +1 418 659 39 25, email aattouri@aln.ulaval.ca
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Abstract

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The effect of feeding lactic-acid bacteria on indices of functions of lymphocytes obtained from Peyer's patches, peripheral blood and spleen from inbred Wistar-Furth rats were studied. Rats were fed on purified diets supplemented with 350 g milk or yoghurt/kg diet for 4 weeks. At the end of the feeding period, immune cells from the three sites were isolated and proliferation, interferon-γ production and lymphocyte subset composition were studied. Rats consuming yoghurt had a greater in vitro proliferative response to yoghurt bacteria in the three lymphoid compartments, a greater interferon-γ production in response to bacteria and concanavalin A in Peyer's patches and spleen, and a greater number of Peyer's patches B lymphocytes than milk-fed rats. Macrophage and T lymphocyte proportions and lymphocyte subset composition in the three sites were unaffected by yoghurt. These results indicate that feeding live bacteria contained in yoghurt may interact with the intestinal immune system, and influence the systemic immune system.

Keywords

Lactic-acid bacteriaIntestinal and systemic immunityInterferonRats
Type
Research Article
Information
British Journal of Nutrition , Volume 87 , Issue 4 , April 2002 , pp. 367 - 373
Copyright
Copyright © The Nutrition Society 2002

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