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Phytosterol-mediated inhibition of intestinal cholesterol absorption is independent of ATP-binding cassette transporter A1

Published online by Cambridge University Press:  08 March 2007

Laura Calpe-Berdiel ,
Joan Carles Escolà-Gil  and
Francisco Blanco-Vaca
Laura Calpe-Berdiel
Affiliation:
Servei de Bioquímica, Hospital de la Santa Creu i Sant Pau, 08025 BarcelonaSpain Institut de Recerca, Hospital de la Santa Creu i Sant Pau, 08025 Barcelona, Spain
Joan Carles Escolà-Gil*
Affiliation:
Servei de Bioquímica, Hospital de la Santa Creu i Sant Pau, 08025 BarcelonaSpain Institut de Recerca, Hospital de la Santa Creu i Sant Pau, 08025 Barcelona, Spain
Francisco Blanco-Vaca
Affiliation:
Servei de Bioquímica, Hospital de la Santa Creu i Sant Pau, 08025 BarcelonaSpain
*
*Corresponding author: fax +34 93 2919196, email jescola@hsp.santpau.es
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Abstract

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An increased activity of ATP-binding cassette transporter (ABC) A1 has been proposed as a mechanism underlying the hypocholesterolaemic effect of phytosterols. In the present study, ABCA1-deficient mice (ABCA1−/− mice) were used to examine the involvement of the ABCA1 in the reduction of intestinal cholesterol absorption in response to a phytosterol-enriched diet. A decrease in intestinal cholesterol absorption of 39 and 35% was observed after phytosterol treatment in ABCA1+/+ mice and in ABCA1−/− mice, respectively. No statistically significant changes in plasma lipoprotein profile or in intestinal ABCG5, ABCG8 and Niemann-Pick C1-Like 1 gene expression levels were found when phytosterol-treated ABCA1−/− mice and untreated ABCA1−/− mice were compared. We conclude that phytosterol inhibition of cholesterol absorption in mice is independent of ABCA1

Keywords

PhytosterolsIntestinal cholesterol absorptionATP-binding cassette transporter A1Mice
Type
Research Article
Information
British Journal of Nutrition , Volume 95 , Issue 3 , March 2006 , pp. 618 - 622
Copyright
Copyright © The Nutrition Society 2006

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