Hostname: page-component-78c5997874-j824f Total loading time: 0 Render date: 2024-11-14T08:55:52.686Z Has data issue: false hasContentIssue false
  • English
  • Français

Preparation and characterization of a [14C]cellulose suitable for human metabolic studies

Published online by Cambridge University Press:  09 March 2007

M. P. Walters ,
J. Kelleher ,
J. M. Findlay  and
S. T. Srinivasan
M. P. Walters
Affiliation:
Department of Medicine, St James's University Hospital, Leeds LS9 7TF
J. Kelleher
Affiliation:
Department of Medicine, St James's University Hospital, Leeds LS9 7TF
J. M. Findlay
Affiliation:
Department of Gastroenterology, Bradford Royal Infirmary, Bradford
S. T. Srinivasan
Affiliation:
Department of Medicine, St James's University Hospital, Leeds LS9 7TF
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

In six normal subjects administered 5 μCi of an oral dose of a commercially available 14C-labelled cellulose, significant amounts of 14CO2 were detected in expired air within 30 min, suggesting that other 14C-labelled non-cellulosic material was present. Chemical and microscopical examination confirmed that starch was the principal contaminant. The commercial preparation was purified using amyloglucosidase (EC 3.2.1.3) digestion following gelatinization of the starch by autoclaving. Subsequent administration of the purified cellulose to a further six normal subjects decreased the expired air 14CO2 during the subsequent 10 h from 13.0 (sd 4.0) to 4.1 (sd 1.9)%. Administration of the purified product to a further group of four normal subjects, before and after a regimen of increased dietary fibre, showed a cumulative increase in expired 14CO2 over 24 h from 7.9 (sd 1.1) to 12.1 (sd 2.6) % on fibre. In six ileostomy subjects the cumulative excretion of 14CO2 was greatly decreased compared with normal controls (3.0 (sd 1.14) and 10.5 (sd 3.9) % respectively). In constipated subjects expired 14CO2 continued beyond 48 h, in contrast to normal subjects where expired 14CO2 at this time was negligible.

Keywords

Dietary fibreDigestion of celluloseHuman metabolic studies
Type
Research Article
Information
British Journal of Nutrition , Volume 62 , Issue 1 , July 1989 , pp. 121 - 129
Copyright
Copyright © The Nutrition Society 1989

References

REFERENCES

Carryer, P.W., Brown, M.L., Malagelada, J.R., Carlson, G.L. & McCall, J.T. (1982). Quantification of the fate of dietary fiber in humans by a newly developed radiolabeled fiber marker. Gastroenterology 82, 1389–1394.CrossRefGoogle ScholarPubMed
Conrad, H.E., Watts, W.R., Iacono, J.M., Kraybill, H.F. & Friedemann, H.E. (1958). Digestibility of uniformly labelled 14C soya bean cellulose in the rat. Science 127, 1293.CrossRefGoogle Scholar
Cummings, J.H. (1981). Dietary fibre. British Medical Bulletin 37, 65–70.CrossRefGoogle ScholarPubMed
Cummings, J.H. & Englyst, H.N. (1987). Fermentation in the human large intestine and the available substrates. American Journal of Clinical Nutrition 45, 1243–1255.Google Scholar
Cummings, J.H., Southgate,, D.A.T., Branch, W.J., Wiggins, H.S., Houston, H., Jenkins,, D.J.A., Jivraj, T. & Hill, M.J. (1979). The digestion of pectin in the human gut and its effect on calcium absorption and large bowel function. British Journal of Nutrition 41, 477–485.CrossRefGoogle ScholarPubMed
Englyst, H.N. & Cummings, J.H. (1987). Digestion of polysaccharides of potato in the small intestine of man. American Journal of Clinical Nutrition 45, 423–431.CrossRefGoogle ScholarPubMed
Englyst, H.N., Trowell, H., Southgate, D.A.T. & Cummings, J.H. (1987). Dietary fiber and resistant starch. American Journal of Clinical Nutrition 46, 873–874.CrossRefGoogle ScholarPubMed
Findlay, J.M., Hart, G., Srinivasan, S.T., Selby, B. & Davis, K.M. (1981). Observations of 14CO2 breath excretion patterns using an orally administered preparation of 14C-labelled cellulose. British Journal of Radiology 54, 706.Google Scholar
Fromm, H. & Hofmann, A.F. (1971). Breath test for altered bile-acid metabolism. Lancet ii, 621–625.CrossRefGoogle Scholar
Ghose, T.K. & Kostick, J.A. (1970). A model for continuous enzymatic saccharification of cellulose with simultaneous removal of glucose. Biotechnology and Bioengineering 12, 921–946.Google Scholar
Holloway, W.D., Tasman-Jones, C. & Lee, S.P. (1978). Digestion of certain fractions of dietary fiber in humans. American Journal of Clinical Nutrition 31, 927–930.CrossRefGoogle ScholarPubMed
Kelleher, J., Walters, M.P., Srinivasan, T.R., Hart, G., Findlay, J.M. & Losowsky, M.S. (1984). Degradation of cellulose within the gastrointestinal tract in man. Gut 25, 811–815.CrossRefGoogle ScholarPubMed
King, K.W. (1966). Enzymatic degradation of crystalline hydrocellulose. Biochemistry and Biophysical Research Communications 24, 295–298.CrossRefGoogle Scholar
Ladisch, M.R., Hong, J., Voloch, M. & Tsao, G.T. (1981). Cellulase kinetics. Basic Life Sciences 18, 55–83.Google ScholarPubMed
Milton-Thompson, G.J. & Lewis, B. (1971). The breakdown of dietary cellulose in man. Gut 12, A853–A854.Google ScholarPubMed
Mitchell, W.D. & Eastwood, M. (1976). Dietary fiber and colon function. In Fibre in Human Nutrition, pp. 186–192 [Spiller, G.A. and Amen, R.J., editors]. New York: Plenum Press.Google Scholar
Nyman, M., Nils-Gregory, A., Cummings, J.H. & Wiggins, H. (1986). Fermentation of dietary fibre in the intestinal tract: comparison between man and rat. British Journal of Nutrition 55, 487–496.CrossRefGoogle Scholar
Prynne, C.J. & Southgate, D.A.T. (1979). The effects of a supplement of dietary fibre on faecal excretion in human subjects. British Journal of Nutrition 41, 495–503.Google Scholar
Rasper, V.F. (1981). Fractionation of the insoluble residue in dietary fiber analysis. In The Analysis of Dietary Fiber in Food, pp. 29–31 [James, W.P.T. and Theander, O., editors]. New York: Marcel Dekker.Google Scholar
Rautela, G.S. & King, K.W. (1968). Significance of the crystalline structure of cellulose in the production and action of cellulase. Archives of Biochemistry and Biophysics 123, 589–601.CrossRefGoogle Scholar
Southgate, D.A.T. & Durnin, J.V.G.A. (1970). Calorie conversion factors. An experimental reassessment of the factors used in the calculation of the energy value of human diets. British Journal of Nutrition 24, 517–535.CrossRefGoogle ScholarPubMed
Stephen, A.M., Wiggins, H.S. & Cummings, J.H. (1987). Effect of changing transit time on colonic microbial metabolism in man. Gut 28, 601–609.CrossRefGoogle ScholarPubMed
Stephen, A.M., Wiggins, H.S., Englyst, H.N., Cole, T.J., Wayman, B.J. & Cummings, J.H. (1986). The effect of age, sex and level of intake of dietary fibre from wheat on large-bowel function in thirty healthy subjects. British Journal of Nutrition 56, 349–361.CrossRefGoogle ScholarPubMed
Van Soest, P.J. (1973). The uniformity and nutritive availability of cellulose. Federation Proceedings 32, 1804–1808.Google ScholarPubMed
Winchell, H.S., Stahelin, H., Kusubov, N., Slanger, B., Fish, M., Pollycove, M. & Lawrence, J.H. (1970). Kinetics of CO2-HCO3 in normal adult males. Journal of Nuclear Medicine 11, 711–715.Google ScholarPubMed