Hostname: page-component-745bb68f8f-g4j75 Total loading time: 0 Render date: 2025-01-28T05:16:11.809Z Has data issue: false hasContentIssue false
  • English
  • Français

Prevention of colon cancer by pre- and probiotics: evidence from laboratory studies

Published online by Cambridge University Press:  11 January 2019

Bandaru S. Reddy
Bandaru S. Reddy*
Affiliation:
American Health Foundation, Valhalla, New York 10595, USA
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Oligofructose and inulin, selective fermentable chicory fructans, have been shown to stimulate the growth of bifidobacteria which are regarded as beneficial strains in the colon. Studies were designed to evaluate inulin (Raftiline) and oligofructose (Raftilose), for their potential inhibitory properties against aberrant crypt foci (ACF) formation in the colon of rats. ACF are putative preneoplastic lesions from which adenomas and carcinomas may develop. The results of this study demonstrate that dietary administration of oligofructose and inulin inhibits the formation of preneoplastic lesions in the colon suggesting the potential colon tumour inhibitory properties of chicory fructans. Since these prebiotics selectively stimulate the growth of bifidobacteria, tumour inhibitory activity of lyophilized cultures of Bifidobacterium longum (BL) against azoxymethane (AOM)-induced colon carcinogenesis in rats and modulating effect of these cultures on colonic tumour cell proliferation, ornithine decarboxylase (ODC) activity, and ras-p21 oncoprotein expression were investigated. Dietary administration of lyophilized cultures of BL strongly suppressed AOM-induced colon tumour development. Inhibition of colon carcinogenesis was associated with a decrease in colonic mucosal cell proliferation and colonic mucosal and tumour ODC and ras-p21 activities.

Keywords

Colon cancerOligofructoseInulinBifidobacterium longum
Type
Research Article
Information
British Journal of Nutrition , Volume 80 , supplement S2: Pre- and Probiotics: Where are We Today? , October 1998 , pp. S219 - S223
Copyright
Copyright © The Nutrition Society 1998

References

Barbacid, M (1990) Ras oncogenes: their role in neoplasia. European Journal of Clinical Investigation, 20, 225–235.Google Scholar
Biasco, G, Paganelli, GM & Brandi, G (1991) Effect of Lactobacillus acidophilus and Bifidobacterium bifidum on rectal cell kinetics and fecal pH. Italian Journal of Gastroenterology 23, 142.Google Scholar
Campbell, JM, Fahey, GC Jr & Wolf, BW (1997) Selected indigestible oligosaccharides affect large bowel mass, cecal and fecal short-chain fatty acids, pH and micorflora in rats. Journal of Nutrition 127, 130–136.Google Scholar
Gamet, L, DaVaind, D, Denis-Poouxviel, C, Ramsey, C & Murat, JC (1992) Effects of short-chain fatty acids on growth and differentiation of the human colon cancer cell line HT 29. International Journal of Cancer 52, 286–289.Google Scholar
Gibson, GR & Roberfroid, MB (1995) Dietary modulation of the human colon microbiota: introducing the concept of prebiotics. Journal of Nutrition 125, 1401–1412.Google Scholar
Gibson, GR, Beatty, ER, Wang, X & Cummings, JH (1995) Selective stimulation of bifidobacteria in the human colon by oligofructose and inulin. Gastroenterology 108, 975–982.Google Scholar
Hague, A, Manning, AM, Hanlon, KA, Hutschtscha, LI, Hart, D & Paraskeva, C (1993) Sodium butyrate induces apoptosis in human colonic tumor cell lines in p53-independent pathway: implications for possible role of dietary fiber in the prevention of large bowel cancer. International Journal of Cancer 55, 498–505.Google Scholar
Howe, GR, Benito, E, Castello, R et al. (1992) Dietary intake of fiber and decreased risk of cancers of the colon and rectum: evidence from the combined analysis of 13 case–control studies. Journal of the National Cancer Institute 84, 1887–1896.Google Scholar
Kasai, H, Nishimura, S, Wakabayashi, K, Nagao, M, & Sugimura, T (1980) Chemical synthesis of 2-amino-3-methylimidazo [4,5-f]quinoline (IQ), a potent mutagen isolated from broiled fish. Proceedings of the Japanese Academy 56B, 382–384.Google Scholar
Kotsinas, A, Spandidos, DA, Romanowski, P & Wyllie, AH (1993) Relative expression of wild-type and activated Ki-ras2 oncogene in colorectal carcinomas. International Journal of Oncology 3, 841–845.Google Scholar
Kulkarni, N & Reddy, BS (1994) Inhibitory effect of Bifidobacterium longum cultures on the azoxymethane-induced aberrant crypt foci formation and fecal bacterial β-glucuronidase. Proceedings of the Society for Experimental Biology and Medicine 207, 278–283.Google Scholar
Luk, GD & Baylin, SB (1984) Ornithine decarboxylase as biological marker in familial colonic polyps. New England Journal of Medicine, 311, 80–83.Google Scholar
Luk, GD, Zhang, SZ & Hamilton, SR (1989) Effects of timing of administration and dose of difluoromethylornithine on rat colonic carcinogenesis. Journal of the National Cancer Institute 81, 421–427.Google Scholar
McLellan, EA, Medline, A & Brid, RP (1991) Sequential analyses of the growth and morphological characteristics of aberrant crypt foci: putative preneoplastic lesions. Cancer Research 51, 5270–5274.Google Scholar
Mukhopadhyay, T, Tainsky, M, Cavander, AC & Roth, JA (1991) Specific inhibition of K-ras expression and tumorigenicity of lung cancer cells by antisense RNA. Cancer Research 51, 1744–1748.Google Scholar
Parker, SL, Tong, T, Bolden, S & Wingo, PA (1997) Cancer Statistics, 1997. CA-A Cancer J. Clin 47, 5–27.Google Scholar
Pegg, AE & Dolan, ME (1989) Investigation of sequence specificity in DNA alkylation and repair using oligodeoxynucleotide substrate. In DNA Repair Mechanisms and their Biological Implications in Mammalian Cells, pp. 45–59, [Lambart, MW & Laval, J, editors]. Plenum Press, New York.Google Scholar
Porter, CW, Herrera-Ornelas, L, Clark, J, Pera, P, Petrelli, NJ & Mittleman, A (1987) Polyamine biosynthetic activity in normal and neoplastic human colorectal tissue. Cancer (Philadelphia) 60, 1275–1281.Google Scholar
Pretlow, TP, O'Riordan, MA, Pretlow, TG & Stellato, TA (1992) Aberrant crypts in human colonic mucosa: putative preneo-plastic lesions. Journal of Cellular Biochemistry 16G (Suppl.), 55–62.Google Scholar
Reddy, BS (1995) Dietary fiber and colon cancer: animal model studies. Preventative Medicine 6, 559–565.Google Scholar
Reddy, BS & Rivenson, A (1993) Inhibitory effect of Bifidobacterium longum on colon, mammary and liver carcinogenesis induced by 2-amino-3-methylimidazo[4,5-f]quinoline, a food mutagen. Cancer Research 53, 3914–3918.Google Scholar
Reddy, BS, Engle, A, Simi, B & Goldman, M (1992) Effect of dietary fiber on colonic bacterial enzymes and bile acids in relation to colon cancer. Gastroenterology 102, 1475–1482.Google Scholar
Reddy, BS, Hamid, R & Rao, CV (1997) Effect of dietary oligofructose and inulin on colonic preneoplastic aberrant crypt foci inhibition. Carcinogenesis 18, 1371–1374.Google Scholar
Sekine, K, Ohta, J, Onishi, M et al. (1995) Analysis of antitumor properties of effector cells stimulated with a cell wall preparation (WPG) of Bifidobacterium infantis . Biology Pharmaceuticals Bulletin 18, 148–153.Google Scholar
Singh, J, Kelloff, G & Reddy, BS (1992) Effect of chemopreventive agents on intermediate biomarkers during different stages of azoxymethane-induced colon carcinogenesis. Cancer Epidemiology Biomarkers and Prevention 1, 405–411.Google Scholar
Singh, J, Rivenson, A, Tomita, M, Shimamura, S, Ishibashi, N & Reddy, BS (1997) Bifidobacterium longum, a lactic acid-producing intestinal microflora inhibit colon cancer and modulate the intermediate biomarkers of colon carcinogenesis. Carcinogenesis, (in press).Google Scholar
Sugimura, T, Nagao, M, Kawachi, T et al. (1977) Mutagen-carcinogens in food, with special reference to highly mutagenic pyrolytic products in broiled foods. In Origins of Human Cancer, pp. 1561–1577. [Hiatt, H, Watson, JD & Winsten, JA editors]. Cold Spring Harbor, New York.Google Scholar
Sugimura, T, Wakabayashi, K, Ohgaki, H, Takayama, S, Nagao, MG & Esumi, H (1991) Heterocyclic amines produced in cooked food: unavailable xenobiotics. In Xenobiotics and Cancer, pp. 279–288 [Ernster, L, editor]. Japan Science Society Press, Tokyo.Google Scholar
Vivona, AA, Shpitz, B, Medline, A et al. (1993) K-ras mutations in aberrant crypt foci, adenomas and adenocarcinomas during azoxymethane-induced colon carcinogenesis. Carcinogenesis (London) 14, 1777–1781.Google Scholar
Wang, X & Gibson, GR (1993) Effect of the in vitro fermentation of oligofructose and inulin by bacteria growing in the human large intestine. Journal of Applied Bacteriology, 75, 373–380.Google Scholar
Wargovich, MH, Chen, DD, Jimenez, A et al. (1996) Aberrant crypts as a biomarker for colon cancer: evaluation of potential chemopreventive agents in the rat. Cancer Epidemiology Biomarkers and Prevention, 5, 355–360.Google Scholar