Hostname: page-component-cd9895bd7-p9bg8 Total loading time: 0 Render date: 2024-12-27T09:03:10.261Z Has data issue: false hasContentIssue false
  • English
  • Français

Prolonged feeding of difructose anhydride III increases strength and mineral concentrations of the femur in ovariectomized rats

Published online by Cambridge University Press:  08 March 2007

Rieko Mitamura  and
Hiroshi Hara
Rieko Mitamura
Affiliation:
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kita-ku, Sapporo 060-8589, Japan
Hiroshi Hara*
Affiliation:
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kita-ku, Sapporo 060-8589, Japan
*
*Corresponding author: Dr Hiroshi Hara, fax +81 11 706 2504, email hara@chem.agr.hokudai.ac.jp
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

This study demonstrates that feeding difructose anhydride III (DFAIII) improves bone strength and femoral mineral concentrations in a rat model of oestrogen deficiency. We showed the relationship between Ca, Mg and P absorption and bone characteristics in rats. Two groups of female Sprague-Dawley rats (6 weeks old) underwent bilateral ovariectomy (ovariectomized rats, OVX rats) or bilateral laparotomy (sham rats). At 10 weeks old, OVX and sham rats were divided into three subgroups and fed a control, 1·5 % DFAIII or 3 % DFAIII diet for 8 weeks, respectively. Ca but not Mg absorption rates were lowered by ovariectomy; however, ingestion of the 1·5 % and 3 % DFAIII diets similarly restored the reduced Ca absorption in OVX rats at 4 and 8 weeks after feeding of the test diets. DFAIII increased Mg absorption dose-dependently in sham and OVX rats. The bone strength, femoral Ca and Mg concentrations, and distal bone mineral density in the 3 % DFAIII group were higher than those in the control group in OVX rats. The absorption rates of Ca and Mg were significantly correlated with femoral Ca and Mg concentrations and strength, which suggests that increasing both Ca and Mg absorption improves bone characteristics in OVX rats. There were no differences in any of the variables in the femur between the 1·5 % and 3 % DFAIII groups in OVX rats. In conclusion, feeding of a low dose of DFAIII increased intestinal Ca and Mg absorption, and the promotive effect of DFAIII persisted for over 8 weeks. This effect was associated with prevention of ovariectomy-induced osteopenia.

Keywords

Difructose anhydride IIICa absorptionBone strengthOvariectomized rats
Type
Research Article
Information
British Journal of Nutrition , Volume 94 , Issue 2 , August 2005 , pp. 268 - 274
Copyright
Copyright © The Nutrition Society 2005

References

Bergman, I & Loxley, R (1970) The determination of hydroxyproline in urine hydrolysates. Clin Chim Acta 27, 347–349.CrossRefGoogle ScholarPubMed
Breitman, PL, Fonseca, D, Cheung, AM & Ward, WE, (2003) Isoflavones with supplemental calcium provide greater protection against the loss of bone mass and strength after ovariectomy compared to isoflavones alone. Bone 33, 597–605.CrossRefGoogle ScholarPubMed
Creedon, A & Cashman, KD (2001) The effect of calcium intake on bone composition and bone resorption in the young growing rat. Br J Nutr 86, 453–459.CrossRefGoogle Scholar
Duncan, DB (1955) Multiple range and multiple F test. Biometrics 11, 1–42.CrossRefGoogle Scholar
Eastell, R (2003) Management of osteoporosis due to ovarian failure. Med Pediatr Oncol 41, 222–227.CrossRefGoogle ScholarPubMed
Eastell, R & Lambert, H (2002) Diet and healthy bones. Calcif Tissue Int 70, 400–404.CrossRefGoogle ScholarPubMed
Geng, W, DeMoss, DL & Wright, GL (2000) Effect of calcium stress on the skeleton mass of intact and ovariectomized rats. Life Sci 66, 2309–2321.CrossRefGoogle ScholarPubMed
Ginty, F, Flynn, A & Cashman, K (1998) The effect of short-term calcium supplementation on biochemical markers of bone metabolism in healthy young adults. Br J Nutr 80, 437–443.CrossRefGoogle ScholarPubMed
Hara, H, Suzuki, T, Kasai, T, Aoyama, Y & Ohta, A (1999) Ingestion of guar gum hydrolysate, a soluble fiber, increases calcium absorption in totally gastrectomized rats. J Nutr 129, 39–45.CrossRefGoogle ScholarPubMed
Holzherr, ML, Retallack, RW, Gutteridge, DH, et al. (2000) Calcium absorption in postmenopausal osteoporosis: benefit of HRT plus calcitriol, but not HRT alone, in both malabsorbers and normal absorbers. Osteoporos Int 11, 43–51.CrossRefGoogle Scholar
Ilich, JZ & Kerstetter, JE (2000) Nutrition in bone health revisited: a story beyond calcium. J Am Coll Nutr 19, 715–737.CrossRefGoogle Scholar
Kaastad, TS, Reikeras, O, Madsen, JE, Narum, S, Stromme, JH, Obrant, KJ & Nordsletten, L (1997) Effects of clodronate on cortical and trabecular bone in ovariectomized rats on a low calcium diet. Calcif Tissue Int 61 158–164.CrossRefGoogle ScholarPubMed
Kalu, DN & Orhii, PB (1999) Calcium absorption and bone loss in ovariectomized rats fed varying levels of dietary calcium. Calcif Tissue Int 65, 73–77.CrossRefGoogle ScholarPubMed
Kanis, JA (1996) Estrogens, the menopause, and osteoporosis. Bone 19, 185S–190S.CrossRefGoogle ScholarPubMed
Lustgarten, JA & Wenk, RE (1972) Simple, rapid, kinetic method for serum creatinine measurement. Clin Chem 18, 1419–1422.CrossRefGoogle ScholarPubMed
Medeiros, DM, Ilich, J, Ireton, J, Matkovic, V, Shiry, L & Wildman, R (1997) Femurs from rats fed diets deficient in copper or iron have decreased mechanical strength and altered mineral composition. J Trace Elem Exp Med 10, 197–203.3.0.CO;2-8>CrossRefGoogle Scholar
Medeiros, DM, Plattner, A, Jennings, D & Stoecker, B (2002) Bone morphology, strength and density are compromised in iron-deficient rats and exacerbated by calcium restriction. J Nutr 132 3135–3141.CrossRefGoogle ScholarPubMed
Mineo, H, Hara, H, Kikuchi, H, Sakurai, H & Tomita, F (2001) Various indigestible saccharides enhance net calcium transport from the epithelium of the small and large intestine of rats in vitro. J Nutr 131, 3243–3246.CrossRefGoogle ScholarPubMed
Mineo, H, Hara, HShigematsu, N, Okuhara, Y & Tomita, F, (2002) Melibiose, difructose anhydride III and difructose anhydride IV enhance net calcium absorption in rat small and large intestinal epithelium by increasing the passage of tight junctions in vitro. J Nutr 132, 3394–3399.CrossRefGoogle ScholarPubMed
Ministry of Health, Labour and Welfare in Japan (2002) National Nutrition Survey 2000. Tokyo: Daiichi-shuppan.Google Scholar
Mitamura, R, Hara, H, Aoyama, Y & Chiji, H (2002) Supplemental feeding of difructose anhydride III restores calcium absorption impaired by ovariectomy in rats. J Nutr 132, 3387–3393.Google ScholarPubMed
Mitamura, R, Hara, H, Aoyama, Y, Takahashi, T & Furuta, H (2003) Ingestion of water-soluble soybean fiber prevents osteopenia and hypercholesterolemia induced by ovariectomy in rats. J Agric Food Chem 51, 1085–1089.CrossRefGoogle ScholarPubMed
Ohta, A, Ohtsuki, M, Baba, S, Adachi, T, Sakata, T & Sakaguchi, E (1995) Calcium and magnesium absorption from the colon and rectum are increased in rats fed fructooligosaccharides. J Nutr 125, 2417–2424.CrossRefGoogle ScholarPubMed
Okuma, T (2001) Magnesium and bone strength. Nutrition 17, 679–680.CrossRefGoogle ScholarPubMed
O'Loughlin, PD & Morris, HA (1994) Oophorectomy in young rats impairs calcium balance by increasing intestinal calcium secretion. J Nutr 124, 726–731.CrossRefGoogle ScholarPubMed
Oxlund, H, Barckman, M, Ortoft, G & Andreassen, TT (1995) Reduced concentrations of collagen cross-links are associated with reduced strength of bone. Bone 17, 365S–371S.CrossRefGoogle ScholarPubMed
Qu, Q, Zheng, H, Dahllund, J, et al. (2000) Selective estrogenic effects of a novel triphenylethylene compound, FC1271a, on bone, cholesterol level, and reproductive tissues in intact and ovariectomized rats. Endocrinology 141, 809–820.CrossRefGoogle Scholar
Reeves, PG, Nielsen, FH & Fahey, GC Jr (1993) AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123, 1939–1951.CrossRefGoogle Scholar
Roberfroid, MB (1999) Concepts in functional foods: the case of inulin and oligofructose. J Nutr 129, 1398S–1401S.CrossRefGoogle ScholarPubMed
Rude, RK, Gruber, HE, Norton, HJ, Wei, LYFrausto, A & Mills, BG (2004) Bone loss induced by dietary magnesium reduction to 10 % of the nutrient requirement in rats is associated with increased release of substance P and tumor necrosis factor. J Nutr 134, 79–85.CrossRefGoogle ScholarPubMed
Shiga, K, Hara, H, Okano, G, Ito, MMinami, A & Tomita, F (2003) Ingestion of difructose anhydride III and voluntary running exercise independently increase femoral and tibial bone mineral density and bone strength with increasing calcium absorption in rats. J Nutr 133, 4207–4211.CrossRefGoogle ScholarPubMed
Shiga, K, Hara, H, Takahashi, T, Aoyama, Y, Furuta, H & Maeda, H (2002) Ingestion of water-soluble soybean fiber improves gastrectomy-induced calcium malabsorption and osteopenia in rats. Nutrition 18, 636–642.CrossRefGoogle ScholarPubMed
Suzuki, T & Hara, H (2004) Various non-digestible saccharides increase intracellular calcium ion concentration in rat small-intestinal enterocytes. Br J Nutr 92, 751–755.CrossRefGoogle ScholarPubMed
Suzuki, T, Hara, H, Kasai, T & Tomita, F (1998) Effects of difructose anhydride III on calcium absorption in small and large intestines of rats. Biosci Biotechnol Biochem 62, 837–841.CrossRefGoogle ScholarPubMed
Swaminathan, R (2001) Biochemical markers of bone turnover. Clin Chim Acta 313, 95–105.CrossRefGoogle ScholarPubMed
Talbott, SM, Rothkopf, MM & Shapses, SA, (1998) Dietary restriction of energy and calcium alters bone turnover and density in younger and older female rats. J Nutr 128, 640–645.CrossRefGoogle ScholarPubMed
Toba, Y, Kajita, Y, Masuyama, R, Takada, Y, Suzuki, K & Aoe, S (2000) Dietary magnesium supplementation affects bone metabolism and dynamic strength of bone in ovariectomized rats. J Nutr 130, 216–220.CrossRefGoogle ScholarPubMed
Ueda, I & Wada, T (1970) Determination of inorganic phosphate by the molybdovanadate method in the presence of ATP and some interfering organic bases. Anal Biochem 37, 169–174.CrossRefGoogle ScholarPubMed
van den Heuvel, EG, Schoterman, MH & Muijs, T (2000) Transgalactooligosaccharides stimulate calcium absorption in postmenopausal women. J Nutr 130, 2938–2942.CrossRefGoogle ScholarPubMed
Wang, X, Shen, X, Li, X & Agrawal, CM (2002) Age-related changes in the collagen network and toughness of bone. Bone 31, 1–7.CrossRefGoogle ScholarPubMed
Zafar, TA, Weaver, CM, Zhao, Y, Martin, BR & Wastney, ME, (2004) Nondigestible oligosaccharides increase calcium absorption and suppress bone resorption in ovariectomized rats. J Nutr 134, 399–402.Google ScholarPubMed