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Protein synthesis in vivo in rats fed on lipid-rich liquid diets

Published online by Cambridge University Press:  09 March 2007

Ernesto Estornell ,
Teresa Barber  and
José Cabo
Ernesto Estornell
Affiliation:
Departament de Bioquímica i Biologia Molecular, Universitat de Valéncia. Facultat de Farmàcia, Avgda. Vicent Andrés Estellés s/n, 46100-Burjassot (Valéncia), Spain.
Teresa Barber
Affiliation:
Departament de Bioquímica i Biologia Molecular, Universitat de Valéncia. Facultat de Farmàcia, Avgda. Vicent Andrés Estellés s/n, 46100-Burjassot (Valéncia), Spain.
José Cabo
Affiliation:
Departament de Bioquímica i Biologia Molecular, Universitat de Valéncia. Facultat de Medicina i Odontologia, Avgda. Vicent Andrés Estellés s/n, 46100-Burjassot (Valéncia), Spain.
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Abstract

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Changes in tissue composition and protein synthesis ratio were studied in the major tissues of the body in young rats fed on lipid-rich, isonitrogenous purified liquid diets, a convenient method for inducing voluntary overfeeding under controlled nutritional conditions. Overfed rats showed faster growth induced by the energy excess. Analysis of tissue composition (protein, DNA and RNA contents) revealed that growth was due mainly to tissue hyperplasia in which protein and DNA contents increased in parallel. Fractional protein synthesis ratio measured in vivo by the flooding-dose method of phenylalanine showed a marked increase in all tissues. This change could be attributed to an increase in the ribosomal activity for protein synthesis in most tissues. Therefore, our results indicate that addition of a supplementary energy source (as lipids) to a well-balanced diet improves growth and protein synthesis in growing rats.

Keywords

OverfeedingTissue compositionProtein synthesis
Type
Protein synthesis on low-fat diets
Information
British Journal of Nutrition , Volume 72 , Issue 4 , October 1994 , pp. 509 - 517
Copyright
Copyright © The Nutrition Society 1994

References

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