Hostname: page-component-cd9895bd7-mkpzs Total loading time: 0 Render date: 2024-12-28T16:53:04.123Z Has data issue: false hasContentIssue false
  • English
  • Français

Protein v. enzymic protein hydrolysates. Nitrogen utilization in starved rats

Published online by Cambridge University Press:  09 March 2007

Julio J. Boza ,
Olga Martínez-Augustin ,
Luis Baró ,
M. Dolores Suarez  and
Angel Gil
Julio J. Boza
Affiliation:
Department of Biochemistry and Molecular Biology, University of Granada, Spain
Olga Martínez-Augustin
Affiliation:
PULEVA Research and Development, Granada, Spain
Luis Baró
Affiliation:
PULEVA Research and Development, Granada, Spain
M. Dolores Suarez
Affiliation:
Department of Biochemistry and Molecular Biology, University of Granada, Spain
Angel Gil
Affiliation:
PULEVA Research and Development, Granada, Spain
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The present study was carried out to compare the effects of four isoenergetic and isonitrogenous diets on the N utilization, total serum protein concentration and serum amino acid profile in starved rats at weaning. These diets differed only in the molecular form of two milk proteins (whey protein and casein), which were either native or partly hydrolysed. Male Wistar rats at weaning were fasted for 3 d and then refed with one of the four diets for 48 h. No differences were observed in the body weight gain, protein digestibility and total serum protein concentration between groups after the refeeding period and all the N balances were positive. N retention was higher in the two groups of rats given the protein-hydrolysate-based diets compared with those given the intact-protein-based diets. This was associated with a lower urinary N excretion in rats, given the whey-protein-hydrolysate and the casein-hydrolysate diets. Despite this fact, the serum amino acid pattern of rats given the hydrolysed protein diet was very similar to that of those given the corresponding native protein diet. In conclusion, we have proved that enzymic hydrolysates from milk proteins have equivalent effects to native proteins in recovery after starvation in rats at weaning, on N absorption, total serum protein concentration and serum amino acid profile, and even give a higher N retention. We did not observe any harmful effect in using protein hydrolysates instead of native proteins.

Keywords

ProteinNitrogenStarvation
Type
Digesta kinetics and feed intake in silage-fed animals
Information
British Journal of Nutrition , Volume 73 , Issue 1 , January 1995 , pp. 65 - 71
Copyright
Copyright © The Nutrition Society 1995

References

REFERENCES

Association of Official Analytical Chemists (1984) Official Methods of Analysis pp. 303–304Arlington: Association of Official Analytical Chemists.Google Scholar
Boza., J. J. 1992 Obtención de hidrolizados enzimáticos de proteinas lácteas Estudio del valor nutritivo y de la capacidad antigénica. (Obtention of enzymic milk protein hydrolysates Nutritional value and antigenic capacity studies) PhD Thesis, University of Granada Spain.Google Scholar
Brinson, R. R., Hanumanthu, S. K. & Pitts, W. M. (1989) A reappraisal of the peptide-based enteral formulas: applications. Nutrition in Clinical Practice 4, 211–217.Google Scholar
Dixon, W. J., Brown, M. B., Engelman, L & Jennrich, R. I. (1990) BMDP Statistical Software Manua Berkeley: University of California Press.Google Scholar
Firmansyah, A., Suwandito, L., Penn, D. & Lebenthal, E. (1989) Biochemical and morphological changes in the digestive tract of rats after prenatal and postnatal malnutrition. American Journal of Clinical Nutrition 50, 261–268.CrossRefGoogle ScholarPubMed
Grimble, G. K., Keohane, P. P., Higgins, B. E., Kaminski, M. V. & Silk, D.B.A. (1986) Effect of peptide chain length on amino acids and nitrogen absorption from two lactoalbumin hydrolysates in the normal human jejunum. Clinical Science 71, 65–69.CrossRefGoogle Scholar
Grimble, G. K. & Silk, D. B. A. (1986) The optimum form of dietary nitrogen in gastrointestinal disease: proteins, peptides or amino acids. Verhandlungen der Deutschen Gesellschaft für Innere Medizin 92, 674–685.Google Scholar
Grimble, G. K. & Silk, D. B. A. (1989) Peptides in human nutrition. Nutrition Research Reviews 2, 87–108.CrossRefGoogle ScholarPubMed
Hatch, T. F. (1981) Effects of protein-calorie malnutrition on the digestive and absorptive capacities of infants. In Textbook of Gastroenterology and Nutrition in Infancy, pp. 767–777 [Lebenthal, E. editor] New York: Raven Press.Google Scholar
Hoshi, T., Abe, M. & Nunukawa, T. (1988) Physiological mechanisms of intestinal absorption of protein hydrolysates and their relevance to, bacterial growth in small intestine. Bifidobacteria Microflora 7, 29–34.CrossRefGoogle Scholar
Institute for, Laboratory Animal Resources (1979) Control of diets in laboratory animal experimentation. Nutrition Abstracts and Reviews: Livestock Feed and Feeding 49, 413–419.Google Scholar
Iyngkaran, N., Yadav, M. & Boey, C. G. (1991) Intestinal brush border peptidases in cow's milk protein-sensitive enteropathy. Acta Paediatrica Scandinavica 80, 549–550.Google Scholar
Knights, R. (1985) Processing and evaluation on the antigenicity of protein hydrolysates. In Nutrition for Special Needs in Infancy. Protein Hydrolysates, pp. 105–115 [Lifishitz, F. editor] New York: Marcel Dekker.Google Scholar
Linblad, B. S. (1978) Free amino acid diets in the vicious circle of diarrhea-malnutrition-malabsorption during infancy. Acta Paediatrica Scandinavica 67, 393–396.CrossRefGoogle Scholar
McMurray, J. (1988) Plasma proteins. In Practical Clinical Biochemistry, pp. 401–435 [Gowenlock, A. H. editor] Oxford: Heinemann Professional Publishing.Google Scholar
Poullain, M. G., Cezard, J. P., Roger, L. & Mendy, F. (1989) Effect of whey proteins, their oligopeptide hydrolysates and free amino, acid mixtures on growth and nitrogen retention in fed and starved rats. Journal of Parenteral and Enteral Nutrition 13, 382–386.CrossRefGoogle ScholarPubMed
Rigo, J. & Senterre, J. (1987) Significance of plasma amino acid pattern in preterm infants. Biology of the Neonate 52, S41–S49.CrossRefGoogle ScholarPubMed
Rigo, J., Verloes, A. & Senterre, J. (1989) Plasma amino acid concentrations in term infants fed human milk, a whey-predominant formula, or a whey hydrolysate formula. Journal of Pediatrics 115, 752–754.CrossRefGoogle ScholarPubMed
Rouanet, J. M., Zambonino Infante, J. L., Caporiccio, B. & Peojan, C. (1990) Nutritional value and intestinal effects of dipeptides and tripeptides. Annals of Nutrition and Metabolism 34, 175–182.CrossRefGoogle ScholarPubMed
Scholze, T. (1985) Determination of phenylthiocarbamil amino acids by reversed-phase high-performance liquid chromatography. Journal of Chromatography 350, 453–460.Google Scholar
Silk, D. B. A, Fairclough, P. D., Clark, M. L., Hegarty, J. E., Marrs, T. C., Addison, J. M., Burston, D., Clegg, K. M. & Matthews, D. M. (1980) Uses of a peptide rather than a free amino acid nitrogen source in chemically defined elemental diets. Journal of Parenteral and Enteral Nutrition 4, 548–553.Google Scholar
Solomons, N. W., Molina, S. & Bulux, J. (1989) Effect of protein-energy malnutrition on the digestive and absorptive capacities of infants and children. In Textbook of Gastroenterology and Nutrition in Infancy, 2nd ed., pp. 517–533 [Lebenthal, E. editor] New York: Raven Press.Google Scholar